@article { author = {Hashemi, Maryam and Ebrahimian, Mahboubeh}, title = {Recent advances in nanoformulations for co-delivery of curcumin and chemotherapeutic drugs}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {1-7}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8046}, abstract = {The application of chemotherapy in cancer treatment has been limited due to cause side effects such as toxicity against normal cells and drug resistance. In recent years, numerous studies have been focused on using natural products with chemotherapeutic drugs to enhance therapeutic efficiency and reduce cytotoxicity. On the other hand, encapsulation of drugs into nanoparticles (NPs) can improve solubility of hydrophobic drug; circulation time in blood and the residence at the pathological site by enhance permeation and retention (EPR) effect. It has been shown that curcumin (CUR) has  wide range of pharmacological activities against many diseases such as cancer. CUR has been demonstrated to be a potent chemosensitizer that can induce additive or synergistic effects with chemotherapeutic drugs against different cancer cell lines.  Recently, various types of nanocarriers have been investigated for CUR.  In this review, different co-formulations containing Cur and chemotherapeutic drugs used in cancer therapy are discussed with emphasis on their pharmaceutical properties.}, keywords = {Cancer,Curcumine,Co-delivery,Nanoformulation}, url = {https://nmj.mums.ac.ir/article_8046.html}, eprint = {https://nmj.mums.ac.ir/article_8046_3d7869fb31f1aecda99b79a4573c4999.pdf} } @article { author = {Cheraghi, Roya and Alipour, Mohsen and Nazari, Mahboobeh and Hosseinkhani, Saman}, title = {Optimization of conditions for gene delivery system based on PEI}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {8-16}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8047}, abstract = {Objective(s): PEI based nanoparticle (NP) due to dual capabilities of proton sponge and DNA binding is known as powerful tool for nucleic acid delivery to cells. However, serious cytotoxicity and complicated conditions, which govern NPs properties and its interactions with cells practically, hindered achievement to high transfection efficiency. Here, we have tried to optimize the properties of PEI/ firefly luciferase plasmid complexes and cellular condition to improve transfection efficiency. Materials and Methods: For this purpose, firefly luciferase, as a robust gene reporter, was complexed with PEI to prepare NPs with different size and charge. The physicochemical properties of nanoparticles were evaluated using agarose gel retardation and dynamic light scattering.  MCF7 and BT474 cells at different confluency were also transfected with prepared nanoparticles at various concentrations for short and long times. Results: The branched PEI can instantaneously bind to DNA and form cationic NPs. The results demonstrated the production of nanoparticles with size about 100-500 nm dependent on N/P ratio. Moreover, increase of nanoparticles concentration on the cell surface drastically improved the transfection rate, so at a concentration of 30 ng/ìl, the highest transfection efficiency was achieved. On the other side, at confluency between 40-60%, the maximum efficiency was obtained. The result demonstrated that N/P ratio of 12 could establish an optimized ratio between transfection efficiency and cytotoxicity of PEI/plasmid nanoparticles. The increase of NPs N/P ratio led to significant cytotoxicity. Conclusion: Obtained results verified the optimum conditions for PEI based gene delivery in different cell lines.}, keywords = {Anxiety,Nanoparticles,Nociception,Postpartum Depression,Vitamin C}, url = {https://nmj.mums.ac.ir/article_8047.html}, eprint = {https://nmj.mums.ac.ir/article_8047_336bb60bb612e864e2af1989703279be.pdf} } @article { author = {Kesmati, Mahnaz and Zadehdarvish, Farzaneh and Jelodar, Zahra and Torabi, Mozhgan}, title = {Vitamin C potentiate sedative effect of magnesium oxide nanoparticles on anxiety and nociception in the postpartum depression model}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {17-24}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8048}, abstract = {Objective(s): Our previous studies have shown that MgO nanoparticles (MgO NPs) could improve anxiety and reduce pain in animals. In this study, the effect of co administration MgO NPs and vitamin C on anxiety like behavior and nociception in postpartum depression (PPD) model were investigated.Materials and Methods:  Female mice (27 ± 3 g) were divided into groups: two control groups, PPD groups:  saline or MgO NPs 1, 5, 10 mg/kg (acute/ chronic), vitamin C 5 and 25 mg/kg (acute) alone or co-injected with MgO NPs 5 (acute). For induction of PPD, chronic injection of progesterone for 5 days was used and three days after progesterone withdrawn (PWD), the depression behavior was evaluated by tail suspension test. Elevated plus maze and hot plate tests were used for evaluation of anxiety and pain perception, respectively. Results: PWD induced anxiety and acute injection of MgO NPs 5 reduced anxiety while chronic injection increased anxiety (P<0.05). Acute and chronic injections of MgO NPs 10 increased anxiety (P<0.05, PConclusion: Effects of MgO NPs on anxiety like behavior induced by PWD, is depends on dose and usage duration. Probably usage of antioxidant can improve efficacy of MgO NPs on anxiety reduction and nociception.}, keywords = {Anxiety,Nanoparticles,Nociception,Postpartum Depression,Vitamin C}, url = {https://nmj.mums.ac.ir/article_8048.html}, eprint = {https://nmj.mums.ac.ir/article_8048_e6ad1a016fa234665b79a3fb003aa073.pdf} } @article { author = {Amoli-Diva, Mitra and Pourghazi, Kamyar}, title = {Poly (methacrylic acid-co-acrylic acid)-grafted polyvinylpyrrolidone coated Magnetic nanoparticles as a pH-responsive magnetic Nano-carrier for controlled delivery of antibiotics}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {25-36}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8049}, abstract = {Objective(s): Pharmaceutical industries are leading to improved medications that can target diseases more effectively and precisely. Researchers have intended to reformulate drugs so that they may be more safely used in human body. The more targeted a drug is, the lower its chance of triggering drug resistance, a cautionary concern surrounding the use of broad-spectrum antibiotics. The aim of this paper is to introduce efficient drug delivery vehicles which can perform both targeted and controlled antibiotic release by synthesis of magnetic pH-responsive polymer.    Materials and Methods: Iron oxide nanoparticles were synthesized by chemical co-precipitation technique and primary coated with 3-trimethoxysilylpropylamine (APTS). APTS coated MNPs was used in the reaction medium for synthesis of a pH-responsive poly (MAA-co-AAc)-grafted PVP. The prepared vehicle was characterized by TEM, XRD, FT-IR, TGA, DSC and elemental analysis. Drug loading, release, kinetics and mechanism of the system were evaluated.Results: The results for drug release showed that the release of antibiotics in pH 5.5 and 7.2 could be effectively sustained, while about 92 % of the drugs were released at pH 1.2. Considerations demonstrate the tendency of drug release by Fickian mechanism and diffusion controlled release.Conclusion: The results indicate that the prepared magnetic nano-carrier could be suitable for site-specific antibiotic delivery through oral administration.}, keywords = {Ciprofloxacin,Drug Delivery,Fe3O4 nanoparticles,Ofloxacin,pH-responsive polymer}, url = {https://nmj.mums.ac.ir/article_8049.html}, eprint = {https://nmj.mums.ac.ir/article_8049_1ac0d186cebbedfd21db2b5d399954dc.pdf} } @article { author = {Ramezani Ali Akbari, Khadijeh and Abdi Ali, Ahya}, title = {Study of antimicrobial effects of several antibiotics and iron oxide nanoparticles on biofilm producing pseudomonas aeruginosa}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {37-43}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8051}, abstract = {Objective(s): Pseudomonas aeruginosa is a nosocomial pathogen resistant to most antimicrobial treatments. Furthermore, it persists in adverse environments thereby forming biofilms on various surfaces. Researchers have therefore focused on antibiofilm strategies using nanoparticles due to their unique physicochemical properties. Superparamagnetic iron oxide nanoparticles (SIONPs) have recently shown to possess antimicrobial and anti-biofilm characteristics. In this study, the effects of SIONPs and some antibiotics were tested against strong biofilmproducing P. aeruginosa isolates.Materials and Methods: 60 isolates of P.aeruginosa were screened for biofilm formation on microtiter plates using 0.1%w/v crystal violet (CV) staining. Twenty isolates producing strong biofilms were selected for further study on the effects of antimicrobial agents. Microdilution method was used to assay twenty isolates susceptible to antibiotics. The effects of antibiotics and SIONPs on biofilm formation were determined by the microdilution method and 0.1% CV staining. The checkerboard dilution technique was used to determine the combined effects of SIONPs and imipenem.      Results: In twenty isolates, the rate of resistance to ciprofloxacin, levofloxacin, amikacin, azithromycin was 65, 75, 45 and 95% respectively. SIONPs at 30 µg/ml reduced biofilm biomass in 11 isolates; however it stimulated biofilm formation in 9 isolates. The effects of SIONPs in combination with imipenem in the 10 isolates were different exhibiting synergistic or antagonistic relationships.Conclusion: P. aeruginosa has increasingly developed resistance to many antimicrobial agents but the resistance to nanoparticles is less frequently been reported. However, iron oxide nanoparticles could enhance biofilm production in isolate- dependent manner because they may possibly utilize this nanoparticle as an iron source, an important element in biofilm production. The exact mechanism of these effects however, remains to be elucidated.}, keywords = {Antibiotics,Biofilm,Iron oxide nanoparticles,Pseudomonas aeruginosa,Resistance}, url = {https://nmj.mums.ac.ir/article_8051.html}, eprint = {https://nmj.mums.ac.ir/article_8051_c55fd780dbe3ed14f38993850d4f66ad.pdf} } @article { author = {Madadi Mahani, Nosrat}, title = {ONIOM studies of interaction between single-walled carbon nanotube and gallates derivatives as anticancer agents}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {44-49}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8052}, abstract = {Objective(s): The novel 7-hydroxycoumarinyl gallates derivatives are detected in many pharmaceutical compounds like anticancer and antimicrobial agents. Whereas carbon nanotubes (CNTs) have been discussed for nanomedicine applications and in particular as drug delivery systems. The capability of armchair (5, 5) SWCNT -based drug delivery system in the therapy of anticancer has been investigated by quantum mechanics/molecular mechanics method.Materials and Methods: Theoretical investigation of the interaction between armchair (5, 5) SWCNT with gallates derivatives has been fulfilled by quantum mechanics/molecular mechanics (QM/MM) method by ONIOM2 (DFT: UFF) using the program of GAUSSIAN 03 suite.Results: The results derived from this study, demonstrate  that armchair (5, 5) SWCNT has weak interaction that these interactions contain Vander Waals interactions and indicated clearly that these systems have relatively low durability and so armchair (5, 5) SWCNT is appropriate drug delivery that have been investigated for anti-cancer drug.Conclusion: Analysis of ONIOM2 calculations and the interaction energies of the armchair (5, 5) SWCNT and gallates derivatives represented that this carrier can be utilized to improve the biological and anti-cancer activity of gallates derivatives.}, keywords = {Anticancer,Armchair (5,5) SWCNT,Drug Delivery,Gallates derivatives,ONIOM2,Quantum Mechanics/Molecular Mechanics}, url = {https://nmj.mums.ac.ir/article_8052.html}, eprint = {https://nmj.mums.ac.ir/article_8052_adc1aff0e67088ec9aa00de13189e554.pdf} } @article { author = {Dowlatababdi, Farzane Hoseyni and Amiri, Gholamreza and Mohammadi Sichani, Maryam}, title = {Investigation of the antimicrobial effect of silver doped Zinc Oxide nanoparticles}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {50-54}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8053}, abstract = {The antimicrobial effect of metal nanoparticles such as zinc oxide and silver nanoparticles has been taken into great consideration separately during recent years. The useful application of these nanoparticles in the areas of medicine, biotechnology, and professional prevention of microbes motivated us. The aim of this study was to evaluate antibacterial activity properties of silver doped zinc oxide nanoparticles (ZnO: Ag) by synthesizing them.Materials and Methods: The silver doped zinc oxide nanoparticles (ZnO:Ag) were provided with wet chemical method in an aqueous solution, and mercaptoethanol. The physical properties of the sample were investigated with UV, XRD, and TEM techniques. Then, the antibacterial activity of 50 to 3.12 concentrations of the silver doped zinc oxide nanoparticles (ZnO:Ag) was investigated against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and Enterococcus faecalis by well diffusion method. Moreover, the MIC and MBC values of these nanoparticles were assessed by microdilution method.Results: The size of the nanoparticles was obtained as between 12 and 13 nanometers in average. The optical study of the nanoparticles demonstrated that the band gap of the silver doped nanostructures is higher than that of the pure sample. The zone of inhibition diameter in the presence of 50 mg/ml density was 19, 15 and 8 mm against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, respectively.Conclusion: The results showed that silver doped zinc oxide nanoparticles prevented Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, but did not affect Enterococcus faecalis. The zone of inhibition diameter increases as the density of the nanoparticles does.}, keywords = {Antibacterial activity,Enterococcus faecalis,Escherichia coli,Pseudomonas aeruginosa,Staphylococcus aureus}, url = {https://nmj.mums.ac.ir/article_8053.html}, eprint = {https://nmj.mums.ac.ir/article_8053_b4a8fa2b356893d4af6c272578ba9b05.pdf} } @article { author = {Raisi Dehkourdi, Banafsheh and Fatahian, Soheil and Shahanipoor, Kahin}, title = {Synthesis, Characterization and renal toxicity of ZnO and polyethylene glycol Coated ZnO nanoparticles}, journal = {Nanomedicine Journal}, volume = {4}, number = {1}, pages = {55-60}, year = {2017}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2017.8054}, abstract = {Objective(s): The wide scale use of Zinc oxide nanoparticles (ZnO NPs) in the consumer market world makes human beings more prone to the exposure to ZnO nanoparticles and its adverse effects. Therefore, the aim of the present study is to assess renal toxicity potential of ZnO and Polyethylene glycol Coated ZnO Nanoparticles in rat.Materials and Methods: Co-precipitation chemical method was used in order to synthesize ZnO nanoparticles. The synthesized nanoparticles were coated with PEG (Polyethylene glycol) and the coating interactions were investigated by FTIR (Fourier Transform Infrared Spectroscopy). Structural properties of ZnO NPs were evaluated by TEM (Transmission Electron Microscope) and XRD (X Ray Diffraction). Toxicity assessment of ZnO and PEG coated ZnO nanoparticles were studied in rat by intra peritoneal injections during a one-month. Renal factors (Creatinine, Uric acid and Blood Urea Nitrogen) were measured 15 and 30 days post injection.Results: The synthesized nanoparticles were single phase and have spinel structure. Their size distribution was around 18 nm. Some kidney factors were changed due to the injection of both uncoated and coated nanoparticles (especially in groups received concentrations of more than 100 mg per kg of body weight). Renal factors changes were more considerable in groups received ZnO NPs in comparison with those received PEG coated ZnO NPs. Chemical toxicity studies showed that there was no irreversible effect in the groups received  concentrations less than 200 mg/kg (mg per kg of body weight).Conclusion: The results indicated that renal factors were changed during 15 days post injection, especially in groups received high doses (200 mg/kg). The results of measurements 30 days post injection showed less change in comparison with the control and this indicates that there was no irreversible effect on kidney. Moreover, PEG coated nanoparticles were less toxic in comparison with Uncoated ZnO NPs.}, keywords = {Nontoxicity,PEG coated,Renal factors,ZnO nanoparticle}, url = {https://nmj.mums.ac.ir/article_8054.html}, eprint = {https://nmj.mums.ac.ir/article_8054_033c1aa12b4b6cb5c4b96e95c1e48b85.pdf} }