@article { author = {Najafi, Parvin and Kouchakzadeh, Hasan}, title = {BSA nanoparticles loaded with IONPs for biomedical applications: fabrication optimization, physicochemical characterization and biocompatibility evaluation}, journal = {Nanomedicine Journal}, volume = {6}, number = {1}, pages = {55-66}, year = {2019}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2019.06.008}, abstract = {Objective(s): Cancer diagnosis in its early stages of progress, can enhance the efficiency of treatment utilizing conventional therapy methods. Non-biocompatibility of iron oxide nanoparticles (IONPs) has made a big challenge against their usage as a contrast agent. Efficient coverage by biomolecules such as albumin can be a solution to overcome this problem. Herein, albumin-coated IONPs were prepared and the process was optimized using the response surface methodology (RSM) to achieve optimum loading of IONPs in albumin nanoparticles. Materials and Methods: IONPs were incorporated into bovine serum albumin nanoparticles (BSA NPs) matrix, to yield IONPs-BSA NPs. The resulting nanoparticles were characterized physicochemically by scanning electron microscopy (SEM), dynamic light scattering (DLS), inductively coupled plasma optical emission spectrometry (ICP-OES), Fourier transform infrared spectroscopy (FTIR) and vibrating sample magnetometer (VSM). The stability test was conducted over 6 months.Results: Under optimum conditions of 2.28 mg for iron weight and pH 9.21, loading of 7.76% was obtained for the spherical IONPs-BSA NPs with the size of 177±12 nm, PDI of 0.222±0.07 and zeta-potential of -36.4±2.3 mV. These findings revealed that more than 90% and 60% of the particles retained their size over four and six months of storage at 4 °C, respectively. In addition, non-cytotoxicity and hemocompatibility of IONPs-BSA NPs were verified in vitro thereby offering them as a biocompatible contrast agent for cancer diagnosis. Conclusion: The IONPs-BSA NPs developed in this study are promising to be further investigated and functionalized with a ligand to obtain a targetable MRI contrast agent for early cancer diagnosis.}, keywords = {Bovine serum albumin nanoparticles, Iron oxide nanoparticles, Optimum production,Response surface methodology (RSM)}, url = {https://nmj.mums.ac.ir/article_12159.html}, eprint = {https://nmj.mums.ac.ir/article_12159_051118b8f8c6ad7b990d8a0623cfad2f.pdf} }