eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
135
141
10.22038/nmj.2017.8954
8954
مقاله پژوهشی
The effect of mesoporous silica nanoparticles loaded with epirubicin on drug-resistant cancer cells
Mohammad Yahya Hanafi-Bojd
myhanafibojd@bums.ac.ir
1
Legha Ansari
ansaril911@mums.ac.ir
2
Fatemeh Mosaffa
mosaffaf@mums.ac.ir
3
Bizhan Malaekeh-Nikouei
malaekehb@mums.ac.ir
4
Cellular and Molecular Research Center, Department of Pharmacology, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Objective (s): In chemotherapy for cancer treatment, the cell resistance to multiple anticancer drugs is the major clinical problem. In the present study, mesoporous silica nanoparticles (MSNs) were used as a carrier for epirubicin (EPI) in order to improve the cytotoxic efficacy of this drug against the P-glycoprotein (P-gp) overexpressing cell line. Materials and Methods: MSNs with phosphonate groups were synthesized and characterized. The cytotoxicity of the prepared nanoparticles on drug-sensitive human breast cancer cell line (MCF-7) and drug-resistant cancer cells (MCF-7/ADR) was evaluated. Results: The hydrodynamic size of nanoparticles was 98 nm and surface charge was negative. The viability of sensitive MCF-7 and resistant MCF-7/ADR cells after incubation with MSNs containing EPI at concentration of 5 μg/ml was about 75% and 44%. On the other hand, the viability of sensitive and resistant cells after incubation with free EPI at this concentration was about 48% and 60%, respectively. Conclusion: These nanoparticles exhibited suitable drug efficiencies against drug-resistant MCF-7/ADR cells in vitro experiments.
https://nmj.mums.ac.ir/article_8954_f130ec53243b9b5f4e5fdf1e02ed0edc.pdf
Epirubicin
Mesoporous silica nanoparticles
Multi drug resistance
P-glycoprotein
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
142
151
10.22038/nmj.2017.8955
8955
مقاله پژوهشی
Core-shell magnetic pH-responsive vehicle for delivery of poorly water-soluble rosuvastatin
Mitra Amoli-Diva
1
Kamyar Pourghazi
kmrpourghazi@gmail.com
2
Department of Chemistry, Payam Noor University (PNU), Tehran, Iran
Department of Chemistry, Karazmi (Tarbiat Moalem) University, Tehran, Iran
Objective(s): Development of an oral sustained-controlled release vehicle which, slowly releases the drug and maintains an effective drug concentration for a long time is aimed.Materials and Methods: A biodegradable magnetic polymeric drug delivery vehicle, using superparamagnetic iron oxide nanoparticles encapsulating by polyvinylpyrrolidone-block-polyethylene glycol-block-poly methacrylic acid (PVP-PEG-PMAA) was developed for targeted and controlled delivery of rosuvastatin. The carrier was characterized by TEM, XRD, and FT-IR techniques.Results: A typical carrier has about a 9 nm magnetite core, about 20 nm mean diameter with a narrow size distribution. The loading efficiency and pH-controlled release properties of the carrier were examined using a hydrophobic model drug rosuvastatin. Maximum loading efficiency of about 96% and a release amount of 90% of 12 hours were achieved at 37 oC in pH 1.2. While in pH 5.5 and 7.2, release amount of 25% and 37% were obtained respectively.Conclusion: The results indicate that the prepared pH-responsive polymer which covalently coated around magnetic nanoparticles is an efficient carrier with good loading capacity and controlled-release property.
https://nmj.mums.ac.ir/article_8955_cc2671cd1603817be7ee65655809f1ff.pdf
Drug Delivery
Magnetic Nanoparticles
Nano-carrier
pH-responsive polymer
Rosuvastatin
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
152
163
10.22038/nmj.2017.8956
8956
مقاله پژوهشی
A novel fabrication of PVA/Alginate-Bioglass electrospun for biomedical engineering application
Aliasghar Saberi
1
Mohammad Rafienia
m_rafienia@med.mui.ac.ir
2
Elahe Poorazizi
elahpooraziz@gmail.com
3
Department of Tissue Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Department of Biochemistry, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Objecttive (s): Polyvinylalcohol (PVA) is among the most natural polymers which have interesting properties such as nontoxic nature, biodegradability and high resistance to bacterial attacks making it applicable for tissue scaffolds, protective clothing, and wound healing.Materials and Methods: In the current work, PVA and Na-Alginate nanocomposite scaffolds were prepared using the electrospinning (ELS) technique in an aqueous solution. Also, (5% and 10%) addition of bioglass (BG) ceramic to the nanocomposite scaffold were investigated. The blended nanofibres are characterized by scanning electron microscopy (SEM), Fourier-transform infrared (FTIR), also the bioactivity evaluation of nanocomposite scaffold performed in simulated body fluid (SBF) solutions.Results: The FTIR analysis indicated that PVA and Alginate may have H+ bonding interactions. The results revealed that with a higher amount of BG, a superior degradation as well as a higher chemical and biological stability could be obtained in the nanobiocomposite blend fibres. Furthermore, the blend nanofibre samples of 10% BG powders exhibit a significant improvement during bioactivity and mechanical testing.Conclusion: The increasing water-contact angle on the polymer surface with decreasing PVA and Alginate content indicated that the scaffold were more hydrophobic than were PVA molecules. Also, In addition, the average diameter of fibers in the sample with 10% BG have the highest porosity compared to the other scaffold samples.
https://nmj.mums.ac.ir/article_8956_89e0c2ff39f6ea31f30c50de22d70fab.pdf
Alginate
Bioglass
Electrospinning
Polymer
Tissue engineering
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
164
169
10.22038/nmj.2017.8957
8957
مقاله پژوهشی
The effect of graphite sources on preparation of Photoluminescent graphene nano-sheets for biomedical imaging
Soroush Moasses Ghafary
soroush_moasses@yahoo.com
1
Shadie Hatamie
shadyhatamy@gmail.com
2
Maryam Nikkhah
m_nikkhah@modares.ac.ir
3
Saman Hosseinkhani
saman_h@modares.ac.ir
4
Department of Nanobiothechnology, University of Tarbiat Modares, Tehran, Iran
Institute for Nanoscience and Nanotechnology (INST), Sharif University of Technology,Tehran, Iran
Department of Nanobiothechnology, University of Tarbiat Modares, Tehran, Iran
Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
Objective(s): Graphene as two-dimensional (2D) materials have attracted wide attention in different fields such as biomedical imaging. Ultra-small graphene nano-sheets (UGNSs) have been designated as low dimensional graphene sheets with lateral dimensions less than few nanometres (≤ 500 nm) in one, two or few layers. Several studies have proven that the process of acidic exfoliation and oxidation is one of the most effective methods to synthesize low dimensional graphene sheets. The band gap of graphene can be changed through changing the reaction temperature resulting in different photoluminescent colors. The aim of our study is synthesis of multi-color photoluminescent UGNSs for biomedical imaging.Materials and Methods: Two different UGNSs were synthesized from two different graphite sources via acidic treatment with a mixture of sulfuric and nitric acids. The prepared UGNSs were characterized by UV-Vis, photoluminescent, Raman spectroscopy and scanning electron microscopy (SEM). The photoluminescence colors of the prepared UGNSs were detected under excitation wavelength of 470 nm using optical filters.Results: The results showed that the graphite primary source is a determinant factor in the synthesis of different UGNSs. While altering reaction temperature didn't significantly change the emission wavelengths; however it affected their photoluminescent emission intensity.Conclusion: Overall, nontoxic UGNSs synthesized by simple acidic treatment of graphite with different photoluminescent colors (green, yellow and red) can be a promising fluorescent probe for bioimaging.
https://nmj.mums.ac.ir/article_8957_f076aa0c2f67c948221721064cb4b404.pdf
Bioimaging
Graphite source
Photoluminescent
Ultra-small graphene nano-sheets
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
170
176
10.22038/nmj.2017.8958
8958
مقاله پژوهشی
Cytotoxicity effects of synthesized ZnO and Zn0.97X0.03O (X=Li, Na, and K) nanoparticles by the gelatin-based sol-gel method
Ali Khorsand Zak
alikhorsandzak@gmail.com
1
Nanotechnology Laboratory, Esfarayen University of Technology, Esfarayen, North Khorasan, Iran
Objective: In this study we would like to report the synthesis of pure and group I element doping of ZnO nanoparticles (ZnO-NPs) prepared using gelatin. The use of natural polymers for the preparation of the pure and doped nanoparticles can result in achieving low cost and eco-friendly advantages.Materials and Method: Pure and doped ZnO-NPs were obtained at 500 °C and The cytotoxicity of nanoparticles was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenylte-trazolium bromide (MTT) assay. Briefly, neuro2A cells were seeded at a density of 1×104 cells perwellin96-wellplatesand incubatedfor24h.Thereafter, the cells were treated with various concentrations of nanoparticles in the presence of 10% FBS.Results: X-ray diffraction (XRD) analysis revealed wurtzite hexagonal structure for the prepared nanoparticles. No other peaks related to the other compounds are detected which indicate that the doped group one elements have been diffused into ZnO lattice. Field emission scanning electron microscopy (FESEM) showed that the formation of most nanoparticles in nano scale. In vitro cytotoxicity studies on neuro2A cells show the non-toxic effect of concentration below ~250 μg/mL for pure and K doped ZnONPs and ~63 μg/mL for Li and Na doped ZnO-NPs. Conclusion: The results show that the potentials of the prepared doped samples to be used in cancer treatments.
https://nmj.mums.ac.ir/article_8958_998ead091aa754634385d1163fa754fb.pdf
ZnO
Cytotoxicity
Zinc Oxide
Doping
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
177
183
10.22038/nmj.2017.8959
8959
مقاله پژوهشی
Fabrication of hydroxyapatite-baghdadite nanocomposite scaffolds coated by PCL/Bioglass with polyurethane polymeric sponge technique
Ebrahim Karamian
1
Akram Nasehi
akramnasehi21@yahoo.com
2
Saeed Saber-Samandari
3
Amirsalar Khandan
amir_salar_khandan@yahoo.com
4
Advanced Materials Research Center, Faculty of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
Advanced Materials Research Center, Faculty of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
New Technologies Research Center, Amirkabir University of Technology, Tehran, Iran
Young Researchers and Elite Club, Khomeinishahr Branch, Islamic Azad University, Isfahan, Iran
Objecttive (s): Silicate bioceramics like Baghdadite with chemical formula Ca3ZrSi2O9, has attracted the attention of researchers in biomedical field due to its remarkable in-vitro and in-vivo bioactivity and mechanical properties.Materials and Methods: Therefore, in the current study the baghdadite powder with Sol-Gel method was synthesized. Then, hydroxyapatite/Baghdadite (HA/Bagh) scaffolds were prepared by the replacing the polyurethane polymeric sponge technique. Afterwhile, the ceramic scaffolds were sintered at 1150ºC for 3 h. The prepared scaffold was then coated by polycaprolactone/bioglass (PCL/BG) polymer nanocomposite. Results: Bioactivity and biomineralization in the simulated body fluid (SBF) revealed that the nanocomposite scaffolds coate with PCL/BG had significant bioactivity properties. The morophology and microstructure investigation of soaked samples in SBF indicate that bone-like apatite formed on the surfaces. Also, ion release in SBF containing the scaffolds was measured by inductively coupled plasma (ICP) analysis. The nucleation positions of apatite crystals were areas with high silicon containing, Si+4 ion positions.Conclusion: The study indicates that scaffold containing 30 wt. % baghdadite had proper bioactivity behaviordue to its ability to form bone-like apatite on the surface of specimens.
https://nmj.mums.ac.ir/article_8959_6e74748fd9f7ad1dd7b20db746f49618.pdf
Coating
Nanocomposite
Polymer
Polyurethane polymeric sponge technique
Scaffolds
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
184
190
10.22038/nmj.2017.8960
8960
مقاله پژوهشی
Antimicrobial effects of green synthesized silver nanoparticles using Melissa officinalis grown under in vitro condition
Saba Pirtarighat
s.pirtarighat@gmail.com
1
Maryam Ghannadnia
ghannadnia_ma@yahoo.com
2
Saeid Baghshahi
3
Department of Biotechnology, Imam Khomeini International University (IKIU), Qazvin, Iran
Department of Biotechnology, Imam Khomeini International University (IKIU), Qazvin, Iran
Department of Materials Engineering, Imam Khomeini International University (IKIU), Qazvin, Iran
Objective(s): To evaluate the biosynthesis of Ag NPs using plant extract of Melissa officinalis (at the eight leaf stage) grown under in vitro (controlled) condition for the first time.Materials and Methods: Biosynthesis of Ag NPs using plant extract was carried out and formation of Ag NPs confirmed by UV-Visible spectroscopy, X-ray diffraction (XRD), Field Emission Scanning Electron Microscope (FESEM) and Dynamic Light Scattering (DLS). The functional groups of compounds adsorbed on the Ag NPs were identified using Fourier Transform Infrared Spectroscop (FTIR) studies. The antibacterial activity of the Ag NPs was investigated by agar disc diffusion method.Results: The plant extract showed color change in extract from yellow to brown after formation of Ag NPs. The surface Plasmon resonance found at 450 nm confirmed the formation of Ag NPs. FESEM images revealed relatively spherical- shaped of Ag NPs. The biosynthesized Ag NPs were crystalline in nature with mean diameter about 34.64 nm. FTIR results expounded the functional groups of plant extract responsible for the bio-reduction of silver ions and their interaction between them. The obtained nanoparticles showed good inhibitory activity against the Gram positive and Gram negative bacteria.Conclusion: These results suggested that with changes in plants culture condition it may be possible to obtain nanoparticles with desired characteristics.
https://nmj.mums.ac.ir/article_8960_9e8fc04f9d33dc3a52ddaa35a85b9da3.pdf
Bactericidal effects
Biosynthesis
In vitro culture
Melissa officinalis
Silver nanoparticles
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2017-07-01
4
3
191
196
10.22038/nmj.2017.8961
8961
مقاله پژوهشی
Synthesis and characterization of CdO/GrO nanolayer for in vivo imaging
Abbas Pardakhty
drpardakhti@yahoo.com
1
Mohammad Mehdi Foroughi
2
Mehdi Ranjbar
ranjbarmehdi67@yahoo.com
3
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran
Young Researchers and Elite Club, Kerman Branch, Islamic Azad University, Kerman, Iran
Objective(s): Nanomaterials are playing major roles in imaging by delivering large imaging payloads, yielding improved sensitivity. Nanoparticles have enabled significant advances in pre-clinical cancer research as drug delivery vectors. Inorganic nanoparticles such as CdO/GrO nanoparticles have novel optical properties that can be used to optimize the signal-to-background ratio. This paper reports on a novel processing route for preparation of CdO/GrO nanolayer and investigation of its optical properties for application in in vivo targeting and imaging.Materials and Methods: Nanostructures were synthesized by reacting cadmium acetate and graphene powder. The effects ofdifferent parameters such as power and time of irradiation were also studied. Finally, the efficiency of CdO/GrO nanostructures as an optical composite was investigated using photoluminescence spectrum irradiation. CdO/GrO nanostructures were characterized by means of X-ray diffraction (XRD), atomic force microscopy (AFM), scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) and photoluminescence (PL) spectroscopy.Results: According to SEM images, it was found that sublimation temperature had significant effect on morphology and layers. The spectrum shows an emission peak at 523 nm, indicating that CdO/GrO nanolayer can be used for in vivo imaging.Conclusion: The estimated optical band gap energy is an accepted value for application in in vivo imaging using a QD–CdO/GrO nanolayer.
https://nmj.mums.ac.ir/article_8961_42e1f1988e03ec34fced013e24518784.pdf
CdO/GrO
Hexagonal nanostructures
In vivo targeting
Optical investigation