eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
63
70
10.7508/nmj.2014.02.001
1699
Combined application of sub-toxic level of silver nanoparticles with low powers of 2450 MHz microwave radiation lead to kill Escherichia coli in a short time
Bardia Varastehmoradi
1
Reza Davoodi
2
Eswarapriya Balu
3
Jegatheesan Kalirajan
4
Bijan Zare
bizhanzarea@yahoo.com
5
Ahmad Reza Shahverdi
shahverd@sina.tums.ac.ir
6
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research center, Tehran
Iran Nanotechnology Initiative Council, Tehran, Iran
Department of Biotechnology, St. Michael College of Engineering and Technology, Kalayarkoil, India
Department of Biotechnology, St. Michael College of Engineering and Technology, Kalayarkoil, India
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research center, Tehran
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research center, Tehran University of Medical Sciences, Tehran, Iran
Objective(s):Electromagnetic radiations which have lethal effects on the living cells are currently also considered as a disinfective physical agent. Materials and Methods: In this investigation, silver nanoparticles were applied to enhance the lethal action of low powers (100 and 180 W) of 2450 MHz electromagnetic radiation especially against Escherichia coli ATCC 8739. Silver nanoparticles were biologically prepared and used for next experiments. Sterile normal saline solution was prepared and supplemented by silver nanoparticles to reach the sub-inhibitory concentration (6.25 μg/mL). Such diluted silver colloid as well as free-silver nanoparticles solution was inoculated along with test microorganisms, particularly E. coli. These suspensions were separately treated by 2450 MHz electromagnetic radiation for different time intervals in a microwave oven operated at low powers (100 W and 180 W). The viable counts of bacteria before and after each radiation time were determined by colony-forming unit (CFU) method. Results: Results showed that the addition of silver nanoparticles significantly decreased the required radiation time to kill vegetative forms of microorganisms. However, these nanoparticles had no combined effect with low power electromagnetic radiation when used against Bacillus subtilis spores. Conclusion: The cumulative effect of silver nanoparticles and low powers electromagnetic radiation may be useful in medical centers to reduce contamination in polluted derange and liquid wastes materials and some devices.
https://nmj.mums.ac.ir/article_1699_84086862121e162826b40c668a162930.pdf
Electromagnetic Radiation
Silver nanoparticles
disinfection process
combined effect
*Corresponding
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
71
78
10.7508/nmj.2014.02.002
1700
Synthesis and study of structural and magnetic properties of superparamagnetic Fe3O4@SiO2 core/shell nanocomposite for biomedical applications
Mitra Helmi Rashid Farimani
mi.farimani@gmail.com
1
Nasser Shahtahmassebi
nasse@um.ac.ir
2
Mahmoud Rezaee Roknabadi
roknabad@um.ac.ir
3
Narges Ghows
n_ghows@yahoo.com
4
Department of Physics, Ferdowsi University of Mashhad, Mashhad, Iran
Department of Physics, Ferdowsi University of Mashhad, Mashhad, Iran
Department of Physics, Ferdowsi University of Mashhad, Mashhad, Iran
Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran
Objective(s): This paper describes coating of magnetite nanoparticles (MNPs) with amorphous silica shells. Materials and Methods: First, magnetite (Fe3O4) NPs were synthesized by co-precipitation method and then treated with stabilizer molecule of trisodium citrate to enhance their dispersibility. Afterwards, coating with silica was carried out via a sol-gel approach in which the electrostatically stabilized MNPs were used as seeds. The samples were characterized by means of X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy and vibrating sample magnetometer (VSM). Results: The results of XRD analysis implied that the prepared nanocomposite consists of two compounds of crystalline magnetite and amorphous silica that formation of their core/shell structure with the shell thickness of about 5 nm was confirmed by TEM images. The magnetic studies also indicated that produced Fe3O4@SiO2 core/shell nanocomposite exhibits superparamagnetic properties at room temperature. Conclusion: These core/shell structure due to having superparamagnetic property of Fe3O4 and unique properties of SiO2, offers a high potential for many biomedical applications.
https://nmj.mums.ac.ir/article_1700_5874b720aacd1aba0aa90aa6a8a2181f.pdf
Magnetite
Silica
Core-shell structure
Superparamagnetism
Biomedical applications
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
79
87
10.7508/nmj.2014.02.003
1701
Sliver nanoparticles accelerate skin wound healing in mice (Mus musculus) through suppression of innate immune system
Mohammad Saeed Heydarnejad
m_heydarnejad@yahoo.com
1
Samira Rahnama
samira_bashool@yahoo.com
2
Mohsen Mobini-Dehkordi
mmobinid@gmail.com
3
Parisa Yarmohammadi
4
Hoori Aslnai
5
Research Institute of Biotechnology, Shahrekord University, Iran
Department of Biology, Shahrekord University, Iran
Department of Genetics, Shahrekord University, Iran
Department of Biology, Shahrekord University, Iran
Deptarment of Biology, Islamic Azad University of Shahrekord, Iran
Objective(s): This study aimed to find the effects of silver nanoparticles (Ag-NPs) (40 nm) on skin wound healing in mice Mus musculus when innate immune system has been suppressed.Materials and Methods: A group of 50 BALB/c mice of about 8 weeks (weighting 24.2±3.0 g) were randomly divided into two groups: Ag-NPs and control group, each with 25 mice. Once a day at the same time, a volume of 50 microliters from the nanosilver solution (10ppm) was applied to the wound bed in the Ag-NPs group while in the untreated (control) group no nanosilver solution was used but the wound area was washed by a physiological solution. The experiment lasted for 14. Transforming growth factor beta (TGF-β), complement component C3, and two other immune system factors involving in inflammation, namely C-reactive protein (CRP) and rheumatoid factor (RF) in sera of both groups were assessed and then confirmed by complement CH50 level of the blood. Results: The results show that wound healing is a complex process involving coordinated interactions between diverse immunological and biological systems and that Ag-NPs significantly accelerated wound healing and reduce scar appearance through suppression of immune system as indicated by decreasing levels of all inflammatory factors measured in this study. Conclusion: Exposure of mice to Ag-NPs can result in significant changes in innate immune function at the molecular levels. The study improves our understanding of nanoparticle interaction with components of the immune system and suggests that Ag-NPs have strong anti-inflammatory effects on skin wound healing and reduce scarring.
https://nmj.mums.ac.ir/article_1701_86bdb644e9cac8d3464ac56fa35d39a4.pdf
Silver nanoparticles (Ag-NPs)
Skin wound
Innate immune system
Mus musculus
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
88
93
10.7508/nmj.2014.02.004
1703
Neuronal toxicity of biopolymer-template synthesized ZnO nanoparticles
Majid Darroudi
majiddarroudi@gmail.com
1
Zahra Sabouri
zahra_sabouri68@yahoo.com
2
Reza Kazemi Oskuee
reza.oskuee@gmail.com
3
Hadi Kargar
h.kargar@pnu.ac.ir
4
Hasan Ali Hosseini
shahosseini57@gmail.com
5
Nuclear Medicine Research Center, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Chemistry Department, Payame Noor University, 19395-4697 Tehran, Iran
Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Chemistry Department, Payame Noor University, 19395-4697 Tehran, Iran
Chemistry Department, Payame Noor University, 19395-4697 Tehran, Iran
Objective(s): A simple and "green" method was developed for preparing zinc oxide nanoparticles (ZnO-NPs) in aqueous starch solutions. Starch was used as a stabilizer to control of the mobility of zinc cations and then control growth of ZnO-NPs prepared via a sol-gel method. Because of the special structure of the starch, it permits termination of the particle growth.Materials and Methods: The dried gel was calcined at different temperatures of 400, 500, 600, and 700 °C. The prepared ZnO-NPs were characterized by different techniques such as X-ray diffraction analysis (XRD), transmittance electron microscopy (TEM), and UV-Vis absorption. Results: The XRD results displayed hexagonal (wurtzite) crystalline structure for prepared ZnO nanoparticles with mean sizes below than 50 nm. In vitro cytotoxicity studies on neuro2A cells showed a dose dependent toxicity with non-toxic effect of concentration below 6 μg/mL.
Discussion: The results showed that starch is an eco-friendly material that can be used as a stabilizing agent in the sol-gel technique for preparing of ZnO-NPs in a large scale.
https://nmj.mums.ac.ir/article_1703_dcbf057c77770fd122369dc6e3020477.pdf
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
94
99
10.7508/nmj.2014.02.005
1705
Synthesis of silver nanoparticle using Portulaca oleracea L. extracts
Gholamreza Asghari
1
Jaleh Varshosaz
varshosaz@pharm.mui.ac.ir
2
Nafiseh Shahbazi
shahbazi@yahoo.com
3
Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Dept. of Pharmaceutics, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Objective(s): To evaluate the influences of aqueous extracts of plant parts (stem, leaves, and root) of Portulaca oleracea L. on bioformation of silver nanoparticles (AgNPs).Materials and Methods: Synthesis of silver nanoparticles by different plant part extracts of Portulaca oleracea L. was carried out and formation of nanoparticles were confirmed and evaluated using UV-Visible spectroscopy and AFM.Results:The plant extracts exposed with silver nitrate showed gradual change in color of the extract from yellow to dark brown. Different silver nanoperticles were formed using extracts of different plant parts. Conclusion: It seems that the plant parts differ in their ability to act as a reducing and capping agent.
https://nmj.mums.ac.ir/article_1705_90299603213d8f5082abb164221b846f.pdf
Nanoparticles
Silver
Portulaca oleracea
Plant part
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
100
106
10.7508/nmj.2014.02.006
1707
A novel label-free cocaine assay based on aptamer-wrapped single-walled carbon nanotubes
Sahar Taghavi
1
Sara Ayatollahi
2
Mona Alibolandi
3
Parirokh Lavaee
4
Mohammad Ramezani
5
Khalil Abnous
abnouskh@mums.ac.ir
6
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Objective(s): This paper describes a selective and sensitive biosensor based on the dissolution and aggregation of aptamer wrapped single-walled carbon nanotubes. We report on the direct detection of aptamer–cocaine interactions, namely between a DNA aptamer and cocaine molecules based on near-infrared absorption at λ807. Materials and Methods: First a DNA aptamer recognizing cocaine was non-covalently immobilized on the surface of single walled carbon nanotubes and consequently dissolution of SWNTs was occurred. Vis-NIR absorption (A807nm) of dispersed, soluble aptamer-SWNTs hybrid, before and after incubation with cocaine was measured using a CECIL9000 spectrophotometer. Results: This carbon nanotube setup enabled the reliable monitoring of the interaction of cocaine with its cognate aptamer by aggregation of SWNTs in the presence of cocaine. Disscusion: This assay system provides a mean for the label-free, concentration-dependent, and selective detection of cocaine with an observed detection limit of 49.5 nM.
https://nmj.mums.ac.ir/article_1707_3eaf94081a3aee0637c823a04d0b842d.pdf
Aptamer
Cocaine, Single-walled carbon nanotube, Near infrared absorption
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
107
111
10.7508/nmj.2014.02.007
1708
Synthesis of new dental nanocomposite with glass nanoparticles
Marzieh Monfared
1
Shamseddin Mirdamadi
2
Alireza Khavandi
3
School of Metallurgy and Materials Engineering , Iran University of Science and Technology (IUST), Tehran, Iran
School of Metallurgy and Materials Engineering , Iran University of Science and Technology (IUST), Tehran, Iran
School of Metallurgy and Materials Engineering , Iran University of Science and Technology (IUST), Tehran, Iran
Objective(s): The aim of this study was to synthesis new dental nanocomposites reinforced with fabricated glass nanoparticles and compare two methods for fabrication and investigate the effect of this filler on mechanical properties. Materials and Methods: The glass nanoparticles were produced by wet milling process. The particle size and shape was achieved using PSA and SEM. Glass nanoparticles surface was modified with MPTMS silane. The composite was prepared by mixing these silane-treated nanoparticles with monomers. The resin composition was UDMA /TEGDMA (70/30 weight ratio). Three composites were developed with 5, 7.5 and 10 wt% glass fillers in each group. Two preparation methods were used, in dispersion in solvent method (group D) glass nanoparticles were sonically dispersed in acetone and the solution was added to resin, then acetone was evaporated. In non-dispersion in solvent method (group N) the glass nanoparticles were directly added to resin. Mechanical properties were investigated included flexural strength, flexural modulus and Vickers hardness. Results: Higher volume of glass nanoparticles improves mechanical properties of composite. Group D has batter mechanical properties than group N. Flexural strength of composite with 10%w filler of group D was 75Mpa against 59 Mpa of the composite with the same filler content of group N. The flexural modulus and hardness of group D is more than group N. Conclusion: It can be concluded that dispersion in solvent method is the best way to fabricate nanocomposites and glass nanoparticles is a significant filler to improve mechanical properties of dental nanocomposite.
https://nmj.mums.ac.ir/article_1708_ddc3c1c4113c87fe22a723602a365ee8.pdf
Glass nanoparticles
Dispersion
ِ ِDental nanocomposites
Mechanical properties
eng
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
2014-01-01
1
2
112
120
10.7508/nmj.2014.02.008
1709
In vitro transdermal delivery of propranolol hydrochloride through rat skin from various niosomal formulations
Eskandar Moghimipour
1
Anayatollah Salimi
2
Hassan Dagheri
3
Nanotechnology research center, Jundishapur University of Medical Sciences, Ahvaz, Iran
Nanotechnology research center, Jundishapur University of Medical Sciences, Ahvaz, Iran
Department of Pharmaceutics, School of Pharmacy, Jundishapur University of Medical Sciences, Ahvaz, Iran
Objective(s): The purpose of the present study was to prepare and to evaluate a novel niosome as transdermal drug delivery system for propranolol hydrochloride and to compare the in vitro efficiency of niosome by either thin film hydration or hand shaking method. Materials and Methods: Niosomes were prepared by Thin Film Hydration (TFH) or Hand Shaking (HS) method. Propranolol niosomes were prepared using different surfactants (span20, 80) ratios and a constant cholesterol concentration. In vitro characterization of niosomes included microscopical observation, size distribution, laser light scattering evaluation, stability of propranolol niosomes and permeability of formulations in phosphate buffer (pH=7) through rat abdominal skin. Results: The percentage of entrapment efficiency (%EE) increased with increase in surfactant concentration in all formulations. Among them, F3 formulation (containing span80:cholesterol ratio of 3:1) showed the highest entrapment efficiency (86.74±2.01%), Jss (6.33μg/cm2.h) and permeability coefficient (7.02×10-3cm/h). By increasing the percentage of entrapment efficiency (resulting in increase in surfactant concentration), the drug released time is not prolonged. Among all the formulations, F4 needed more time for maximum drug release. Among these formulations, F4 was also found to have the maximum vesicle size as compared to other formulations. It was observed that niosomal suspension prepared from span 80 was more stable than span 20. Conclusion: This study demonstrates that niosomal formulations may offer a promise transdermal delivery of propranolol which improves drug efficiency and can be used for controlled delivery of propranolol.
https://nmj.mums.ac.ir/article_1709_130982fbe8b065250b28291278743ef1.pdf