%0 Journal Article %T Spectroscopic investigation on the interaction of DNA with superparamagnetic iron oxide nanoparticles doped with chromene via dopamine as cross linker %J Nanomedicine Journal %I Mashhad University of Medical Sciences %Z 2322-3049 %A Dehghan, Gholamreza %A Mehdipour, Maryam %A Hosseinpour Feizi, Mohammad Ali %A Tarasi, Roghayeh %A Khoobi, Mehdi %A Dastmalchi, Siavoush %D 2018 %\ 01/01/2018 %V 5 %N 1 %P 36-45 %! Spectroscopic investigation on the interaction of DNA with superparamagnetic iron oxide nanoparticles doped with chromene via dopamine as cross linker %K DNA interaction %K Dopamine %K Iron oxide nanoparticles %K Spectroscopy %R 10.22038/nmj.2018.05.006 %X Objective(s): The interaction of DNA with iron oxide nanoparticles (SPIONs) was studied to find out the interaction mechanism and design new drug delivery systems. Materials and Methods: The interaction of calf thymus DNA (ctDNA) with SPIONs doped with 2H-chromene via dopamine as cross linker (SPIONs@DA-Chr) was studied using the UV absorption spectroscopy, viscosity measurement, circular dichroism, fluorescence and FT-IR spectroscopic techniques. Results: UV absorption study showed hyperchromic effect in the spectra of DNA. Few changes were observed in the viscosity of ctDNA in the presence of different concentration of SPIONs@DA-Chr. The result of circular dichroism (CD) suggested that SPIONs@DA-Chr can change the secondary structure of DNA. Further, fluorescence quenching reaction of ctDNA with SPIONs@DA-Chr and competitive fluorescence spectroscopy studied by using methylene blue, have shown that the SPIONs@DA-Chr can bind to ctDNA through non-intercalative mode. FT-IR spectroscopy confirmed the binding of SPIONs@DA-Chr and ctDNA. Conclusion: These results suggested that SPIONs@DA-Chr binds to DNA via groove binding mode. %U https://nmj.mums.ac.ir/article_10053_b5a6ddeb18fb8a6e171accd0d79370a4.pdf