%0 Journal Article %T The cellular uptake of antisense oligonucleotid of E6 mRNA into cervical cancer cells by DOPE-modified hydroxyapatite nanoparticles %J Nanomedicine Journal %I Mashhad University of Medical Sciences %Z 2322-3049 %A Saffarzadeh, Negin %A Kalantar, Seyed Mehdi %A Jebali, Ali %A Hekmatimoghaddam, Seyed Hossein %A Sheikhha, Mohammad Hassan %A Farashahi, Ehsan %D 2014 %\ 10/01/2014 %V 1 %N 5 %P 292-297 %! The cellular uptake of antisense oligonucleotid of E6 mRNA into cervical cancer cells by DOPE-modified hydroxyapatite nanoparticles %K Cervical cancer cells %K DOPE %K Hydroxyapatite nanoparticles %K Oligonucleotide %R 10.7508/nmj.2015.05.001 %X Objective(s):  Although several chemical and physical methods for gene delivery have been introduced, their cytotoxicity, non-specific immune responses and the lack of biodegradability remain the main issues. In this study, hydroxyapatite nanoparticles (NPs) and 1,2-dioleoyl-sn-glycero-3-phosphoethanol​amine (DOPE)-modified hydroxyapatite NPs was coated with antisense oligonucleotide of E6 mRNA, and their uptakes into the cervical cancer cell line were evaluated.   Materials and Methods:  Calcium nitrate and diammonium phosphate were used for the synthesis of the hydroxyapatite nanoparticle. Thus, they were coated with polyethylene glycol (PEG), DOPE and antisense oligonucleotide of E6 mRNA using a cross-linker. Then, hydroxyapatite NPs and DOPE-modified hydroxyapatite NPs were incubated 48 hours with cervical cancer cells and their uptakes were evaluated by fluorescent microscopy.   Results:  The hydroxyapatite NPs had different shapes and some agglomeration with average size of 100 nm. The results showed DOPE-modified hydroxyapatite NPs had higher uptake than hydroxyapatite NPs (P<0.05).   Conclusions:  Hydroxyapatite NPs conjugated with DOPE are a good choice for gene delivery and silencing of viral genes in cervical cancer cells, but their efficacy should be addressed more in future studies. %U https://nmj.mums.ac.ir/article_3053_712d8e10ea7a3c3580a0e5b9395de880.pdf