TY - JOUR ID - 10054 TI - Comparative characterization of silver nanoparticles synthesized by spore extract of Bacillus subtilis and Geobacillus stearothermophilus JO - Nanomedicine Journal JA - NMJ LA - en SN - 2322-3049 AU - Ghasemi, Seyed Mahdi AU - Dormanesh, Banafshe AU - Hosseini Abari, Afrouzossadat AU - Aliasghari, Azam AU - Farahnejad, Zohreh AD - Department of Medical Mycology, AJA University of Medical Sciences, Tehran, Iran AD - Department of Pediatric Nephrology, AJA University of Medical Sciences, Tehran, Iran AD - Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran AD - Department of Biology, Faculty of Science, University of Isfahan, Isfahan, Iran. AD - Department of Medical Mycology, AJA University of Medical Sciences, Tehran,Iran Y1 - 2018 PY - 2018 VL - 5 IS - 1 SP - 46 EP - 51 KW - Bacillus subtilis KW - Geobacillus stearothermophilus KW - Silver nanoparticles KW - Spore extract DO - 10.22038/nmj.2018.05.007 N2 - Objective(s): Silver nanostructures have gathered remarkable attention due to their applications in diversefields. Researchers have recently demonstrated that bacterial spores are capable of reducing silver ions toelemental silver leading to formation of nanoparticles.Materials and Methods: In this study, spores of Bacillus subtilis and Geobacillus stearothermophilus wereemployed to produce silver nanoparticles (SNPs) from silver nitrate (AgNO3) through a green synthesismethod. The production of SNPs by spores, heat inactivated spores (microcapsule) and spore extracts wasmonitored and compared at wavelengths between 300 to 700 nm. The biosynthesized SNPs by spore extractswere characterized and confirmed by XRD and TEM analyses.Results: UV-Visible spectroscopy showed that the spore extracts were able to synthesize more SNPs thanthe other forms. The XRD pattern also revealed that the silver nanometals have crystalline structure withvarious topologies. The TEM micrographs showed polydispersed nanocrystal with dimensions ranging from30 to 90 nm and 15 to 50 nm produced by spore extracts of B. subtilis and G. stearothermophilus, respectively.Moreover, these biologically synthesized nanoparticles exhibited antimicrobial activity against differentopportunistic pathogens.Conclusion: This study suggests the bacterial spore extract as a safe, efficient, cost effective and eco-friendlymaterial for biosynthesis of SNPs. UR - https://nmj.mums.ac.ir/article_10054.html L1 - https://nmj.mums.ac.ir/article_10054_e28a5564a79e7906baa0b1884d97cd20.pdf ER -