TY - JOUR ID - 2752 TI - Comparison of nano-hydroxyapatite productivity by Pseudomonas aeruginosa and Serratia marcescense through encapsulation method JO - Nanomedicine Journal JA - NMJ LA - en SN - 2322-3049 AU - Khanafari, Anita AU - Akbari, Tayebeh AU - Sohrabi, Mahmoud Reza AD - Microbiology Department, North Tehran Branch, Islamic Azad University, Tehran, Iran AD - Chemistry Department, North Tehran Branch, Islamic Azad University, Tehran, Iran Y1 - 2014 PY - 2014 VL - 1 IS - 4 SP - 276 EP - 284 KW - Alginate beads KW - Hydroxylapatite KW - Nanoscale KW - Pseudomonas aeruginosa KW - Serratia marcescens DO - 10.7508/nmj.2015.04.008 N2 - Objective(s):   The production of nano-hydroxyapatite by two encapsulated bacterial strains was the goal of current research.  Materials and Methods:   Serratia marcscens ATCC 14756 and Pseudomonas aeruginosa PTCC 1570 were used by two methods including encapsulated form in 2% (w/v) alginate sodium powder and inoculated form (10%) in nutrient broth medium containing alginate sodium blank beads. In both cases alginate beads transferred to calcium and phosphorus precursors mineral medium for 48 h and were incubated at 32-35 °C for 14 days. To obtain hydroxyapatite powder, alginate beads were dried at 60 °C and rubbed. Sol-gel as chemical method was used for comparing with microbial analysis. The nature of produced powders was evaluated in each step by XRD, FTIR and scanning electron microscopy.  Results:   The results showed that the yield rate of sol-gel method was 18.3% and it was much more than encapsulated method (3.032 and 3.203 w/w dried alginate bead). The size of the particles in microbial method were smaller (8-68 nm cylindrical particles and 12-55 and 15-37 nm spherical particles) than chemical method (350-880 nm of cylindrical and 34-67 nm of spherical particles).  Conclusion:   Nanoparticle sizes and distribution of microbial nano-hydroxyapatite powder samples shows that it has excellent physical properties similar to natural bone and may be to produce dense and porous bioactive bone implants with desired properties. UR - https://nmj.mums.ac.ir/article_2752.html L1 - https://nmj.mums.ac.ir/article_2752_db2f6aa64b8c2d6d7fc3dc8a465224b8.pdf ER -