TY - JOUR ID - 3053 TI - The cellular uptake of antisense oligonucleotid of E6 mRNA into cervical cancer cells by DOPE-modified hydroxyapatite nanoparticles JO - Nanomedicine Journal JA - NMJ LA - en SN - 2322-3049 AU - Saffarzadeh, Negin AU - Kalantar, Seyed Mehdi AU - Jebali, Ali AU - Hekmatimoghaddam, Seyed Hossein AU - Sheikhha, Mohammad Hassan AU - Farashahi, Ehsan AD - Department of Medical Genetic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran AD - Department of Medical Genetic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran AD - Department of Laboratory Sciences, Faculty of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Y1 - 2014 PY - 2014 VL - 1 IS - 5 SP - 292 EP - 297 KW - Cervical cancer cells KW - DOPE KW - Hydroxyapatite nanoparticles KW - Oligonucleotide DO - 10.7508/nmj.2015.05.001 N2 - Objective(s):  Although several chemical and physical methods for gene delivery have been introduced, their cytotoxicity, non-specific immune responses and the lack of biodegradability remain the main issues. In this study, hydroxyapatite nanoparticles (NPs) and 1,2-dioleoyl-sn-glycero-3-phosphoethanol​amine (DOPE)-modified hydroxyapatite NPs was coated with antisense oligonucleotide of E6 mRNA, and their uptakes into the cervical cancer cell line were evaluated.   Materials and Methods:  Calcium nitrate and diammonium phosphate were used for the synthesis of the hydroxyapatite nanoparticle. Thus, they were coated with polyethylene glycol (PEG), DOPE and antisense oligonucleotide of E6 mRNA using a cross-linker. Then, hydroxyapatite NPs and DOPE-modified hydroxyapatite NPs were incubated 48 hours with cervical cancer cells and their uptakes were evaluated by fluorescent microscopy.   Results:  The hydroxyapatite NPs had different shapes and some agglomeration with average size of 100 nm. The results showed DOPE-modified hydroxyapatite NPs had higher uptake than hydroxyapatite NPs (P<0.05).   Conclusions:  Hydroxyapatite NPs conjugated with DOPE are a good choice for gene delivery and silencing of viral genes in cervical cancer cells, but their efficacy should be addressed more in future studies. UR - https://nmj.mums.ac.ir/article_3053.html L1 - https://nmj.mums.ac.ir/article_3053_712d8e10ea7a3c3580a0e5b9395de880.pdf ER -