A novel wound rinsing solution based on nano colloidal silver

Authors

1 Biomaterial Group, Medical Engineering Department, AmirKabir University of Technology, Tehran, Iran

2 ChitoTech Company, Khaghani Building, Somayrh Avenue, Tehran, Iran

3 Shahid Sadughi Medical University, Yazd, Iran

10.7508/nmj.2015.05.005

Abstract

Objective(s):
The present study aimed to investigate the antiseptic properties of a colloidal nano silver wound rinsing solution to inhibit a wide range of pathogens including bacteria, viruses and fungus present in chronic and acute wounds.
Materials and Methods:
The wound rinsing solution named SilvoSept® was prepared using colloidal nano silver suspension. Physicochemical properties, effectiveness against microorganism including  Staphylocoocous aureus ATCC 6538P, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739, Candida albicans ATCC 10231, Aspergillus niger ATCC 16404, MRSA, Mycobacterium spp., HSV-1 and H1N1, and biocompatibility tests were carried out according to relevant standards.
Results:
X-ray diffraction (XRD) scan was performed on the sample and verify single phase of silver particles in the compound. The size of the silver particles in the solution, measured by dynamic light scattering (DLS) techniqu, ranged 80-90 nm. Transmission electron microscopy (TEM) revealed spherical shape with smooth surface of the silver nanoparticles. SilvoSept® reduced 5 log from the initial count of 107 CFU/mL of Staphylocoocous aureus ATCC 6538P, Pseudomonas aeruginosa ATCC 9027, Escherichia coli ATCC 8739, Candida albicans ATCC 10231, Aspergillus niger ATCC 16404, MRSA, Mycobacterium spp. Further assessments of SilvoSept solution exhibited a significant inhibition on the replication of HSV-1 and H1N1. The biocompatibility studies showed that the solution was non-allergic, non-irritant and noncytotoxic.
Conclusion:
Findings of the present study showed that SilvoSept® wound rinsing solution containing nano silver particles is an effective antiseptic solution against a wide spectrum of microorganism. This compound can be a suitable candidate for wound irrigation.

Keywords


  1. Cavanagh MH, Burrell RE, Nadworny PL. Evaluating antimicrobial efficacy of new commercially available silver dressings. Int Wound J. 2010; 7(5): 394-405.
  2. Mahltig B, Haase H. Comparison of the effectiveness of different silver-containing textile products on bacteria and human cells. J TEXT I. 2012; 103: 1262-1266.
  3. Shastri JP, Rupani MG, Jain RL. Antimicrobial activity of nanosilver-coated socks fabrics against foot pathogens. The Journal of The Textile Institute. 2012; 103: 1234-1243.
  4. Lee HJ, Yeo SY, Jeong SH. Antibacterial effect of nanosized silver colloidal solution on textile fabrics. J Mater Sci. 2003; 38: 2199-2204.
  5. Petica A, Gavriliu S, Lungu M, Buruntea N, Panzaru C. Colloidal silver solutions with antimicrobial properties. Mater Sci Eng B. 2008; 152: 22-27.
  6. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv. 2009; 27(1): 76-83.
  7. Vivekanandhan S, Christensen L, Misra M, Mohanty AK. Green process for impregnation of silver nanoparticles into microcrystalline cellulose and their antimicrobial bionanocomposite films. J Biomater Nanobiotechnol. 2012;  3: 371-376.
  8. Vaidyanathan R, Kalishwaralal K, Gopalram S, Gurunathan S. Nanosilver—The burgeoning therapeutic molecule and its green synthesis. Biotechnol Adv. 2009; 27: 924-937.
  9. Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci. 2009; 145(1-2): 83-96.
  10. Nagajyothi PC, Lee SE, An M, Lee KD. Green synthesis of silver and gold nanoparticles using Lonicera Japonica flower extract. Bull Korean Chem Soc. 2012; 33(8): 2609-2612.
  11. Prabhu S, Poulose EK. Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int Nano Lett. 2012; 2: 32. http://www.inl-journal.com/content/2/1/32.
  12. Chen X, Schluesener H. Nanosilver: a nanoproduct in medical application. Toxicol Lett. 2008; 176(1): 1-12.
  13. Lehninger AL, Nelson DL, Cox M. Lehninger principles of biochemistry. 4th ed. United Kingdom: Palgrave Macmillan Limited; 2004.
  14. Cho KH, Park JE, Osaka T, Park SG. The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochimica Acta. 2005; 51(5): 956-960.
  15. Keen JS, Desai PP, Smith CS, Suk M. Efficacy of hydrosurgical debridement and nanocrystalline silver dressings for infection prevention in type II and III open injuries. Int Wound J. 2012; 9: 7-13.
  16. Rai MK, Deshmukh SD, Ingle AP, Gade AK. Silver nanoparticles: the powerful nanoweapon against multidrug‐resistant bacteria. J Appl Microbiol. 2012; 112(5): 841-852.
  17. Wang X, Zhuang J, Peng Q, Li Y. A general strategy for nanocrystal synthesis. Nature. 2005; 437: 121-124.
  18. Song HY, Ko KK, Oh IH, Lee BT. Fabrication of silver nanoparticles and their antimicrobial mechanisms. Eur Cell Mater. 2006; 11: 58.
  19. Sun Q, Cai X, Li J, Zheng M, Chen Z, Yu CP. Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloids Surf A Physicochem Eng Asp. 2014; 444: 226-231.
  20. Besinis A, De Peralta T, Handy RD. The antibacterial effects of silver, titanium dioxide and silica dioxide nanoparticles compared to the dental disinfectant chlorhexidine on Streptococcus mutans using a suite of bioassays. Nanotoxicology. 2014; 8(1): 1-16.