Biosynthesis of gold nanoparticles using streptomyces fulvissimus isolate

Document Type : Research Paper

Authors

1 Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, Iran

2 Department of Biotechnology, College of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran

Abstract

Objective(s):
In recent years, the biosynthesis of gold nanoparticles has been the focus of interest because of their emerging application in a number of areas such as biomedicine. In the present study we report the extracellular biosynthesis of gold nanoparticles (AuNPs) by using a positive bacterium named Streptomyces fulvissimus isolate U from rice fields of Guilan Province, Iran.
Materials and Methods:
From over 20 Streptomyces isolates collected, isolate U showed high AuNPs biosynthesis activity. To determine its taxonomical identity, its morphology was characterized by scanning electron microscope and partial molecular analysis performed by PCR. In this regard, 16S rDNA of isolate U was amplified using universal bacterial primers FD1 and RP2. The PCR products were purified and sequenced. Sequence analysis of 16S rDNA was then conducted using NCBI BLAST method. In biosynthesis of AuNPs by this bacterium, the biomass of bacterium exposed to the HAuCl4 solution.
Results:
The nanoparticles obtained were characterized by UV-Visible spectroscopy, transmission electron microscopy (TEM) and Energy dispersive X-ray (EDX) spectroscopy and X-ray diffraction spectroscopy (XRD) analyses. Our results indicated that Streptomyces fulvissimus isolateU bio-synthesizes extracellular AuNPs in the range of 20-50 nm.
Conclusions:
This technique of green synthesis of AuNPs by a microbial source may become a promising method because of its environmental safety. Its optimization may make it a potential procedure for industrial production of gold nanoparticles.

Keywords


1. Boisselier E, Astruc D. Gold nanoparticles in nanomedicine: preparation, imaging, diagnostics, therapies and toxicity. Chem Soc Rev. 2009; 38: 1759-1782.
2. Shahidi Bonjar L. Nanogold detoxifying machine” to remove idle nanogold particles from blood stream of cancer patients treated with antibody-nanogold therapeutics. Med hypotheses. 2013; 80(5): 601–605.
3. Groneberg DA, Giersig M, Welte T, Pison U. Nanoparticle-based diagnosis and therapy. Curr Drug Targets. 2006; 7: 643–648.
4. Duraisamy K, Krishnamoorthy S, PB Tirupathi P, Youn Soo C, Yang Soo L. Synthesis, Characterization and In vitro Cytotoxicity of Gold Nanoparticles Using Cultural Filtrate of Low Shear Modeled Microgravity and Normal Gravity Cultured K. pneumonia. Macromol. Res. 2014; 22(5): 487-493.
5. Khadivi FD, Dehnad A, Mojtaba Salouti. Extracellular Biosynthesis of Gold Nanoparticles by Metal Resistance Bacteria: Streptomyces griseus. synth react inorg met-org nano-met chem. 2012; 42: 868–871.
6. Pranav V, Batherinarayanan A, Dilliganesh T, Kumar M, Munusamy C, Baskar G. Biological synthesis and characterization of intracellular gold nanoparticles using biomass of Aspergillus fumigatus. Bull  Mater Sci. 2013; 36(7): 1201–1205.
7. Lowenstam HA. Minerals formed by organisms. Science. 1981;211:1126–30.
8. Nishant S, Mausumi M. Biosynthesis and Characterization of Gold Nanoparticles Using Zooglea ramigera and Assessment of Its Antibacterial Property. Journal of Cluster Science. 2014.
Available from URL: http://www.springer.com/chemistry-/catalysis/journal/10876
9. Huff TB, Tong L, Zhao Y, Hansen MN, Cheng JX, Wei A. Hyperthermic effects of gold nanorods on tumor cells. Nano-medicine. 2007; 2: 125–132.
10. Khabat V, Mansoori GA, Karimi S. Biosynthesis of Silver Nanoparticles by Fungus Trichoderma Reesei. Insciences J. 2011; 1(1): 65-79.
11. Lee JY, Hwang BK. Diversity of antifungal Actinomycetes in various vegetative soils of Korea. Can J Microbiol. 2002; 48: 407-417.
12. Shahidi Bonjar GH. New approaches in screening for antibactrials in plants. Asian Journal Plant Science. 2004; 3: 55-60.
13. Kim HJ, Lee SC, Hwang BK. Streptomyces cheonanensis sp. Nov., a novel streptomycete with antifungal activity. Int J Syst Evol Microbiol. 2006; 56: 471–475.
14. Pasqua AJD, Mishler RE, Ship YL, Dabrowiak JC, Asefa T. Preparation of antibody conjugated gold nanoparticles. Mater. Lett. 2009; 63:1876.
15. Vasanathi P B, Thangavelu D, Vasanth KM, Munusamy C, Baskar G. Biological synthesis and characterization of intracellular gold nanoparticles using biomass of Aspergillus fumigatus. Bull Mat Sci. 2013; 7:1201-1205.
16. Namvar F, Azizi S, Mansour BA, Shameli K, Rosfarizan, Mahdavi M, Paridah Md, Tahir. Green synthesis and characterization of gold nanoparticles using the marine macroalgae Sargassum muticum. Green synthesis and characterization of gold nanoparticles using the marine macroalgae Sargassum Muticum. Research on Chemical Intermediates. 2014
Available from URL:  http://springer.libdl.ir/article/10.1007/s11164-014-1696-4
17. Jannu VG, Thenmozhi M , Kannabiran K , Rajakumar G, Velayutham K, Rahuman AA. Actinobacteria mediated synthesis of gold nanoparticles using Streptomyces sp. VITDDK3 and its antifungal activity. Mater. Lett. 2013; 93: 360–362.