@article { author = {Mirzaei, Mehdi and Esmaeil Akbari, Mohammad and Mohagheghi, Mohammad Ali and Tavangar, Seyed Mohammad and Mehravi, Bita and Shafiee Ardestani, Mehdi}, title = {In vitro and in vivo toxicity and histopathological evaluation of Gd(III)anionic Linear globular dendrimer second-generation G2-C595 nanoprobe}, journal = {Nanomedicine Journal}, volume = {7}, number = {4}, pages = {284-290}, year = {2020}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2020.07.00004}, abstract = {Objective(s): Toxico-histopathological studies are used to assess the toxic impacts of nanoparticles in organism exposure. The present study aimed to evaluate the prospective nano-cytotoxicity impacts of Gd(III)-anionic linear globular dendrimer second-generation G2-C595 (Gd[III] dendrimer G2-C595) contrast nanoprobe in terms of the exposure of many nude mice organs and organisms. In addition, we assessed the potential of the Gd(III)-dendrimer G2-C595 nanoprobe as a novel magnetic resonance imaging (MRI) nano-contrast agent for the human breast cancer cell line (MCF-7) and human embryonic kidney cell line (HEK-293).Materials and Methods: Gadolinium (Gd[III]) was loaded with dendrimer G2 and conjugated with the C595 monoclonal antibody to generate the Gd(III)-dendrimer G2-C595 to determine the impact on MUC1 beneficial cancer tumors. The cytotoxic effects of the Gd(III)-dendrimer G2-C595 nanoprobe on the HEK-293 cells were also investigated in-vitro and in-vivo. In addition, the Gd(III)-dendrimer G2-C595 nanoprobe was used on nude mice bearing the MCF-7 tumors to explore its specific activity against the in-vivo model of cancer.Results: The Gd(III)-dendrimer G2-C595 contrast nanoprobes affected the cytotoxicity of MCF-7, and no in-vivo toxicity was induced in the HEK-293 cells, kidneys, heart, lungs, brain, liver tissues, and other organs.Conclusion: According to the results, the Gd(III)-dendrimer G2 and Gd(III)-dendrimer G2-C595 induced no toxicity in the HEK-293 cells and heart, liver, and brain tissues of mice. In addition, the Gd(III)-dendrimer G2-C595 showed specific anti-action against the in-vivo tumor model. Therefore, the Gd(III)-dendrimer G2-C595 nanoprobe is highly recommended as a novel and effective MR contrast agent and antitumor carrier agent. Furthermore, the Gd(III)-dendrimer G2-C595 nano-sized probes demonstrated excellent biocompatibility and safety with no impact on normal organ functioning.}, keywords = {Gd(III)-Dendrimer G2-C595 Nanoprobe,Human Embryonic Kidney Cells (HEK-293),human breast cancer cell line (MCF-7),Nanoparticle Toxicity}, url = {https://nmj.mums.ac.ir/article_15841.html}, eprint = {https://nmj.mums.ac.ir/article_15841_0f79cff3fb3db0cb7970675b6ba0828a.pdf} }