@article { author = {Shamasi, Zahra and Es-haghi, Ali and Taghavizadeh Yazdi, Mohammad Ehsan and Amiri, Mohammad Sadegh and Homayouni-Tabrizi, Masoud}, title = {Role of Rubia tinctorum in the synthesis of zinc oxide nanoparticles and apoptosis induction in breast cancer cell line}, journal = {Nanomedicine Journal}, volume = {8}, number = {1}, pages = {65-72}, year = {2021}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2021.08.07}, abstract = {Objective(s): Nowadays, nanotechnology has offered great success in resolving concerns in cancer therapy and created a new interdisciplinary field of study incorporating various sciences, such as biology, chemistry and medicine. Apoptosis is a conserved and controlled strategy in regulating cellular growth and proliferation, as well as preserving development and general homeostasis of the body. Zinc oxide nanoparticles (ZnO-NPs) are the most important and widely used nanoparticles. This study aimed to evaluate the apoptosis-inducing properties of the synthesized ZnO-NPs by aqueous extract of Rubia tinctorum against the MCF7 breast cancer cell line. Materials and Methods: Zinc oxide nanoparticles were synthesized using Rubia tinctorum extract and characterized by some methods including dynamic light scattering (DLS), field emission scanning electron microscopy (FESEM) and x-ray diffraction analysis (XRD). Apoptosis was measured by the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry. Results: The results of this study showed that the particle size of biosynthesized ZnO-NPs using R.tinctorum extract was about 40 nm and had a spherical morphology. The obtain results of the Hoechst and Acridine-Orange/Propodium Iodide staining, as well as flow cytometry showed that biosynthesized ZnO-NPs effectively and dose-dependently induced apoptosis in the MCF7 breast cancer cells.Conclusion: Therefore, the biosynthesized ZnO-NPs by watery extract of R. tinctorum can be used in the treatment of many diseases, including cancers.}, keywords = {Apoptosis,Breast Cancer,Green synthesis,Rubia tinctorum,Zinc oxide nanoparticles}, url = {https://nmj.mums.ac.ir/article_16848.html}, eprint = {https://nmj.mums.ac.ir/article_16848_fcd460b9be325c08a36c596080dcead4.pdf} }