@article { author = {Daneshmand, Sara and Yazdian-Robati, Rezvan and Jaafari, Mahmoud Reza and Movafagh, Jebraeel and Malaekeh-Nikouei, Bizhan and Iranshahi, Mehrdad and Golmohammadzadeh, Shiva and Tayarani-Najaran, Zahra}, title = {Evaluation of the anti-melanogenic activity of nanostructured lipid carriers containing auraptene: A natural anti-oxidant agent}, journal = {Nanomedicine Journal}, volume = {9}, number = {1}, pages = {57-66}, year = {2022}, publisher = {Mashhad University of Medical Sciences}, issn = {2322-3049}, eissn = {2322-5904}, doi = {10.22038/nmj.2022.62354.1645}, abstract = {Objective(s): In this work, we loaded Auraptene (AUR) into nanostructured lipid carriers (NLCs) and performed an assessment on inhibitory activities of the obtained AUR-NLCs on melanogenesis. Materials and Methods: AUR-NLCs were prepared through a high shear homogenization and ultrasound method. Results: Entrapment efficiency and Particle size of the optimized formulation were 103.1±4.9 nm and 89.56±3.75. The TEM outcomes exhibited the spherical shape of our nanoparticles, while the DSC analysis revealed the lack of any drug-lipid incompatibility throughout the formulations. A prolonged drug-release was observed from AUR-NLCs when compared to the AUR-solution. According to results, this product can significantly attenuated the activity of cellular tyrosinase and ROS content with minimal cytotoxic effects in B16F10 cell line, which in contrast to AUR-solution. Moreover, the western blotting analysis was indicative of AUR-NLCs ability to inhibit melanogenesis through the suppression of MITF and act much more efficiently than AUR-solution.Conclusion: AUR-NLCs can offer merits as a natural anti-tyrosinase agent for the treatment of hyperpigmentory disorders.}, keywords = {Anti-tyrosinase,Auraptene,Melanin,Melanogenesis,NLC}, url = {https://nmj.mums.ac.ir/article_19402.html}, eprint = {https://nmj.mums.ac.ir/article_19402_84e2efe0615cc0d1fee3b1d48c1f37a5.pdf} }