Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
An update on the new achievements in the nanocapsulation of anthocyanins
87
97
EN
Davoud
Salarbashi
Nanomedicine Research Center, Gonabad University of Medical Sciences, Gonabad, Iran
davoud.salarbashi2@gmail.com
Javad
Bazeli
Department of Emergency Medicine, School of Nursing and Midwifery, Gonabad University of Medical Sciences, Gonabad, Iran
bazeli@yahoo.com
Elham Fahmideh
Rad
Department of Applied Sciences, Applied Chemistry Section, Higher College of Technology (HCT), Muscat, Sultanate of Oman
10.22038/nmj.2020.07.001
Natural food pigments are commonly utilized for the improvement of the qualitative properties of foods and/or inhibit the development of chronic and degenerative diseases. Several studies have documented the beneficial health effects of natural food pigments, such as anthocyanins, chlorophylls, and carotenoids. These effects mainly depend on the stability, bioactivity, and bioavailability of these pigments. Various techniques have been used to encapsulate natural pigments. Anthocyanins are a member of flavonoid groups, which are responsible for attractive food colors. Due to the positive surface charge of anthocyanin molecules, they absorb light and gain color. The micro- and nano-encapsulation of ingredients using natural polymers are important techniques to improve their stability, solubility, and bioavailability. This review study aimed to elaborate on the recent advancement in the encapsulation of anthocyanin as an attractive natural pigment using five techniques, including coacervation, spray drying, liposomal system, electrospraying, and microwave-assisted encapsulation methods.
Anthocyanins,Bioavailability,Bioactivity,Nano-encapsulation,Polymers
https://nmj.mums.ac.ir/article_14628.html
https://nmj.mums.ac.ir/article_14628_65ed42cc9194c86541fc915d166250c4.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
Polymer-based nanoadjuvants for hepatitis C vaccine: The perspectives of immunologists
98
107
EN
Piyachat
Evelyn Roopngam
Visiting Professor, Department of Biotechnology, University of Verona, Veneto, Italy
Tirawat
Wannatung
Faculty of Medical Technology, Western University, Kanchanaburi, Thailand, 71170
nettirawat@gmail.com
10.22038/nmj.2020.07.002
The hepatitis C virus (HCV) is an infection that affects the liver tissues in humans, leading to the development of effective prophylactic and therapeutic HCV vaccines to prevent a global epidemic. Scientists consider it challenging to produce a therapeutic vaccine for the treatment of hepatocellular carcinoma as opposed to a preventative vaccine. However, several drawbacks are involved with a peptide vaccine, including the low immunogenicity of the protein, significant instability, difficulty in delivery, and inefficient presentation of the antigens. Therefore, the investigation of adjuvants (i.e., immunomodulators) to enhance the efficacy of the vaccine is essential. Nanoparticles could potentially serve as vaccine delivery vehicles, acting as adjuvants for the effective transfer of antigens. The safety and effectiveness of nanoparticles and liposomes in modern vaccinology have also been confirmed. Biodegradable nanopolymers such as polyesters, polylactic acid and the copolymers, polyorthoesters, polyanhydrides, and polycarbonates are commonly used owing to their proper qualities in the combination or loading for the prevention of the degradation of the delivered antigens. The present study is specifically focused on the polymer-based nanoparticles that are mostly comprised a poly (amino acid) based copolymer and poly (D, L-lactic-co-glycolide), which could act as adjuvants or potential immunomodulators for the systems providing effective HCV vaccine delivery.
Adjuvants,HCV,Nanoparticles,Vaccine
https://nmj.mums.ac.ir/article_14867.html
https://nmj.mums.ac.ir/article_14867_c1e0f5224b38c87b9a56f29b20d31e12.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
Docetaxel delivery using folate-targeted liposomes: in vitro and in vivo studies
108
114
EN
Roghayyeh
Vakili-Ghartavol
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Mahmoud Reza
Jaafari
0000-0003-3908-6828
Department of Pharmaceutical Nanotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
jafarimr@mums.ac.ir
Amin Reza
Nikpoor
0000-0002-6289-3794
Molecular Medicine Research Center Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
nikpoora@gmail.com
Seyed Mahdi
Rezayat
https://orcid.org/00
Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
rezayat@tums.ac.ir
10.22038/nmj.2020.07.003
Objective(s): Folate-targeted liposomes have been well considered in folate receptor (FR) overexpressing cells including MCF-7 and 4T1 cells in vitro and in vivo. The objective of this study is to design an optimum folate targeted liposomal formulations which show the best liposome cell uptake to tumor cells.<br />Material and Methods: In this study, we prepared and characterized different targeted formulations and a nontargeted form as a control. Physicochemical analysis showed that the liposomes had homogeneous population and appropriate size to accumulate to tumor sites through the enhanced permeation and retention (EPR) mechanism. Moreover, we compared the cell uptake of folate targeted liposomal docetaxel compared to nontargeted liposomes in vitro. <br />Results: The in vitro drug release profile of the formulations at different time points showed none of the formulations did not has burst release. However, targeted liposomes accumulated in tumor tissue in vivo less than nontargeted formulations which could be attributed to their uptake by RES due to relatively greater size of targeted formulations. It is presumable that analyze the biodistribution process at longer time points and the molecular mechanisms behind the tissue accumulation could clear the issue. <br />Conclusion: We conclude that success in vitro studies holds the promise of folate targeting strategy and in vivo study merits further investigations.
Docetaxel Encapsulation,Folate Targeting,Liposomes,Tumor Drug Delivery
https://nmj.mums.ac.ir/article_14009.html
https://nmj.mums.ac.ir/article_14009_ad19994e2e45b9b5d59a39602af7bcb5.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
In vitro and In vivo Investigation of poly(lactic acid)/hydroxyapatite nanoparticle scaffold containing nandrolone decanoate for the regeneration of critical-sized bone defects
115
123
EN
Majid
Salehi
Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
msalehi.te1392@gmail.com
Arman
Ai
School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
arman.ai@gmail.com
Arian
Ehterami
Department of Mechanical and Aerospace Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran
arian.ehterami@srbiau.ac.ir
Masoumeh
Einabadi
Department of Biology, Islamic Azad University, Jahrom Branch, Jahrom, Iran
Alireza
Taslimi
Dentistry Student, Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
alirezata13@gmail.com
Armin
Ai
0000-0001-8417-5913
Dentistry Student, Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
arianehterami@yahoo.com
Hamta
Akbarzadeh
Dentistry Student, Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
hakbarzadeh@student.tums.ac.ir
Ghazal Jabal
Ameli
Dentistry Student, Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
Saeed
Farzamfar
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran
saeed.tums1991@gmail.com
Sadegh
Shirian
Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
shirian@gmail.com
Nahal
Azimi
Dentistry Student, Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
majidsalehiezd@yahoo.com
Faezeh
Sadeghi
Dentistry Student, Scientific Research Center, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
salehim@shmu.ac.ir
Naghmeh
Bahrami
Craniomaxillofacial Research Center, Tehran University of Medical Sciences, Tehran, Iran
n-bahrami@sina.tums.ac.ir
Arash
goodarzi
Department of Tissue Engineering, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran
dvm.goodarzi86@yahoo.com
Jafar
Ai
Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran 1417755469, Iran
10.22038/nmj.2020.07.004
Objective(s): Bone tissue engineering is aimed at the fabrication of bone graft to ameliorate bone defects without using autografts or allografts. <br />Materials and Methods: In the present study, the coprecipitation method was used to prepare hydroxyapatite (HA) nanoparticles containing nandrolone. To do so, 12.5, 25, and 50 mg of nandrolone were loaded into poly(lactic acid) (PLA)/nano-HA, and the freeze casting method was used to fabricate porous scaffolds. The morphology, mechanical strength, wettability, porosity, degradation, blood compatibility, and cellular response of the scaffolds were evaluated using various tests. For further investigation, the developed scaffolds were incorporated into the rat calvaria defect model, and their effects on bone healing were evaluated. <br />Results: The obtained results indicated that the fabricated scaffolds had the approximate porosity of 80% and compress strength of 6.5 MPa. Moreover, the prepared scaffolds had appropriate hydrophilicity, weight loss, and blood compatibility. Furthermore, the histopathological findings demonstrated that the defects filled with the PLA/nano-HA scaffolds containing 25 mg nandrolone healed better compared to the other study groups.<br />Conclusion: Therefore, it was concluded that the scaffolds containing nandrolone could be used in bone regeneration.
Bone Healing,Freeze Casting Method,Hydroxyapatite,Nandrolone,Scaffold
https://nmj.mums.ac.ir/article_14202.html
https://nmj.mums.ac.ir/article_14202_84d69c22bd3bc015aa9d918fbb45e4e9.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
Synergistic cellular toxicity and uptake effects of iodixanol conjugated to anionic linear globular dendrimer G2
124
130
EN
Mitra
Kiani
Department of Radiopharmacy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
mitrakiani@yahoo.com
Paria
Mojarrad
School of Pharmacy, Tehran University of Medical Sciences, International Branch, Tehran, Iran
pariamojarrad@yahoo.com
Mehdi
Shafiee Ardestani
https://orcid.org/00
Department of Radiopharmacy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
shafieeardestani@gmail.com
10.22038/nmj.2020.07.005
Objective(s): Early diagnosis of cancer using noninvasive imaging techniques has been discussed in several recent studies. The present study aimed to assess the synergistic effects of iodixanol-conjugated polyethylene glycol (PEG)-citrate (anionic linear globular) dendrimer G2 on MCF-7 breast cancer cells and human embryonic kidney 293 (HEK293) cells. <br />Materials and Methods: PEG-citrate dendrimer G2 was synthesized and purified. The product was characterized using atomic force microscopy (AFM), electron energy loss spectroscopy (EELS), dynamic light scattering (DLS). At the next stage, the product was conjugated to iodixanol, purified and lyophilized. The cytotoxic effects of the iodixanol, plain PEG-citrate dendrimer G2, and iodixanol-PEG-citrate dendrimer G2 complex were evaluated using methylthiazole-tetrazolium (MTT) assay on the MCF-7 and HEK293 cells. Inductively coupled plasma mass spectrometry (ICP MS) is a mass spectrometry technique, which applies inductively coupled plasma to ionize samples.<br />Results: According to the obtained results, the uptake of PEG-citrate dendrimer G2 iodixanol increased significantly compared to iodixanol alone (P<0.05), indicating the importance of lack of significant in-vitro toxicity. Moreover, in the particle size and higher negative zeta potential confirmed the loading of iodixanol in dendrimer G2. Increase, the loading of iodixanol in dendrimer was confirmed by the chemical shifts in HNMR. <br />Conclusion: Therefore, it was concluded that the addition of anionic linear globular dendrimer G2 to iodixanol affected the cellular uptake of the drug with no significant toxicity. Recent findings also confirmed that this novel complex could be applied as an effective cancer imaging agent for molecular biology and molecular imaging applications.
Cellular Toxicity,Iodixanol,Linear Globular Dendrimer
https://nmj.mums.ac.ir/article_14531.html
https://nmj.mums.ac.ir/article_14531_4935da6a8834738aebab4aa9b0a1eac5.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
Effect of the nanoliposomal formulations of rifampin and N-acetyl cysteine on staphylococcus epidermidis biofilm
131
137
EN
Farzaneh
Bazrgari
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
bazrgarif911@mums.ac.ir
Bahman
Khameneh
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
khamenehbagherib@mums.ac.ir
Bibi Sedigheh
Fazly Bazzaz
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Asma
Mahmoudi
School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
mahmoudia912@mums.ac.ir
Bizhan
Malaekeh-Nikouei
0000-0002-1908-9530
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
malaekehb@mums.ac.ir
10.22038/nmj.2020.07.006
Objective(s): Staphylococcus epidermidis is a common cause of medical device-associated infections due to biofilm formation, and its elimination is extremely challenging. Although rifampin efficacy against S. epidermidis biofilms has been confirmed, its use as a single agent may lead to resistance. As such, it is assumed that the combination of rifampin and N-acetylcysteine (NAC) could exert additive effects as a mucolytic agent. The present study aimed to use a liposomal system for the delivery of these compounds to bacterial biofilm.<br />Materials and Methods: Liposomal formulations were prepared using the dehydration-rehydration method and characterized in terms of the size, zeta potential, and encapsulation efficacy. In addition, the ability of various formulations in the eradication of bacterial biofilm and inhibition of biofilm formation was assessed based on the optical density ratio. <br />Results: The zeta potential of the liposomes was positive, and the mean size of these liposomal formulations was less than 200 nanometers. Liposomal rifampin was the most effective formulation against S. epidermidis, and the anti-biofilm activity of most of the formulations was concentration-dependent and time-dependent.<br />Conclusion: According to the results, the rifampin-loaded liposomes were effective against S. epidermidis biofilm formation.
Biofilm,Nanoliposomes,N-acetyl cysteine,Rifampin
https://nmj.mums.ac.ir/article_14574.html
https://nmj.mums.ac.ir/article_14574_e572ab9f30c5001ff9612c72c6252f44.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
A novel three-dimensional printing of electroconductive scaffolds for bone cancer therapy application
138
148
EN
Marjan
Monshi
Advanced Materials Research Centre, Department of Materials Science and Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
marjan.monshi2@iaun.ac.ir
Saeid
Esmaeili
Mechanical Engineering Department, Khomeinishahr Branch, Islamic Azad University, Khomeinishahr, Iran
Amin
Kolooshani
Mechanical Engineering Department, Khomeinishahr Branch, Islamic Azad University, Khomeinishahr, Iran
amin_koloshani2@yahoo.com
Bahareh Kamyab
Moghadas
Department of Chemical Engineering, Shiraz Branch, Islamic Azad University, Shiraz, Iran
Saeed
Saber-Samandari
New Technologies Research Center, Amirkabir University of Technology, Tehran, 15875-4413, Iran
Amirsalar
Khandan
New Technologies Research Center, Amirkabir University of Technology, Tehran, 15875-4413, Iran
amirsalar.khandan@cc.emu.edu.tr
10.22038/nmj.2020.07.007
Objective(s): Tissue engineering aims to achieve a tissue, which has highly interconnected porous microstructure concurrent with appropriate mechanical and biological properties. <br />Materials and Methods: Therefore, the microstructure scaffolds are of great importance in this field. In the present study, an electroconductive poly-lactic acid (EC-PLA) filament used to fabricate a porous bone scaffold. For scaffolds model designed, solid-work software was used. Then, the designed modeled was transferred to simplify 3D to laminated with its G-Code file for fused deposition modeling (FDM) printer to create a scaffold with porosity around 65-75%. Two different shapes were designed and fabricated (cylindrical and cubic shape). The samples were coated with hydroxyapatite (HA) nanoparticle to enhance its chemical stability. In this study, the X-ray diffraction (XRD) confirmed that the EC-PLA is non-crystalized and scanning electron microscopy (SEM) used to present the apatite formation on the surface of porous scaffolds. The compression test, fracture toughness, and hardness were measured. The biological response in the physiological saline was performed to determine the rate of degradation of EC-PLA in phosphate buffer saline (PBS) and the apatite formation in the simulated body fluid (SBF) after 14 days. <br />Results: Finally, the biocompatibility of the porous architecture was monitored using human gum (HuGu) cells. The ABAQUS modeling simulation was used to compare the experimental and analytical results. The obtained results showed that by applying force to both cylindrical and cubic scaffold, the Von Mises Stress (VMS) could withstand the scaffold mentioned above at 9.7-11 MPa. <br />Conclusion: Therefore, it can be concluded that prepared porous scaffolds have a high potential in bone tissue engineering and probably the treatment of tumor-related bone defects as photothermal therapy. The porous EC-PLA scaffold was successfully fabricated and showed appropriate compressive strength (39.14 MPa), with controllable porosity of 60-70 %, which is a suitable candidate for replacing in bone tissues.
Cell culture,Electroconductive Poly lacticacid,Scaffold,Tissue engineering
https://nmj.mums.ac.ir/article_15125.html
https://nmj.mums.ac.ir/article_15125_633cde7eb3bb66c1230af043119c534a.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
Evaluation of protein corona formation and anticancer efficiency of curcumin-loaded zwitterionic silica nanoparticles
149
157
EN
Shokoofeh
Maghari
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
sh.maghari@gmail.com
Alireza
Ghassempour
0000-0001-8540-8846
Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran, Iran
a-ghassempour@sbu.ac.ir
10.22038/nmj.2020.07.008
Objective(s): Study and development of antifouling nanosystem for conjugation of drugs were attracting great attention in recent years. The present study aimed to develop novel curcumin-loaded silica nanoparticles containing zwitterionic coating as an antifouling system to provide protein corona free nanoformulations for curcumin. <br />Materials and Methods: Silica nanoparticles were prepared using the Stöber method, and mono- and bi-functionalized nanoparticles were obtained by modifying the surface of the bare silica nanoparticles with (3-aminopropyl)triethoxysilane (APTES), polyethylene glycol amine, APTES with sulfobetaine, and polyethylene glycol amine with sulfobetaine. Nanoparticle characterization, curcumin release, and measurement of protein corona inhibition were performed after incubation in the human plasma and MTT assay to confirm the stability and efficiency of the nanoparticles. <br />Results: The presence of the sulfobetaine group could influence the curcumin loading capacity of the silica nanoparticles. The results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated no significant protein adsorption on the curcumin-loaded, zwitterionic-coated nanoparticles compared to the other nanoparticles. In addition, the MTT assay confirmed the cytotoxicity of the curcumin-loaded sulfobetaine-APTES-silica nanoparticles on MCF-7 cancer cells.<br />Conclusion: Our findings confirmed the effects of the zwitterionic coating on the physicochemical properties of the nanoparticles. These findings play a key role in the development of novel nanoparticles for drug delivery applications.
Curcumin,Functionalized Nanoparticles,Protein Corona,Silica Nanoparticles,Zwitterionic Coating
https://nmj.mums.ac.ir/article_15123.html
https://nmj.mums.ac.ir/article_15123_eab66abd85cc243358df7cfcde03057e.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
7
2
2020
04
01
The protective effects of curcumin and curmumin nanomicelle against cirrhotic cardiomyopathy in bile duct-ligated rats
158
169
EN
Mohammad
Sheibani
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
mohammad.sheibani89@gmail.com
Ahmad Reza
Dehpour
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Sadaf
Nezamoleslami
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
sadaf.nezamoleslami@yahoo.com
Seyedeh Elaheh
Mousavi
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
mousavielaheh90@gmail.com
Mahmoud Reza
Jafari
Department of Pharmaceutics, Mashhad University of Medical Sciences, Mashhad, Iran
JafariMR@mums.ac.ir
Seyed Mahdi
Rezayat Sorkhabadi
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
epouyan@hotmail.com
10.22038/nmj.2020.07.009
Objective(s): Cirrhotic cardiomyopathy refers to cardiac muscle dysfunction caused by liver cirrhosis. Seemingly, free radicals and inflammatory factors play a critical role in the pathophysiology of cardiomyopathy. Curcumin has the anti-inflammatory, antioxidant, and anticancer properties . However, the therapeutic indications of this compound are limited due to its low absorption, rapid metabolism, and low bioavailability. Curcumin nanomicelle is a form of nanoparticle developed to overcome the poor kinetic profile of curcumin and enhance its bioavailability and therapeutic effects. The present study aimed to develop an experimental model of cirrhosis induced by biliary duct ligation in rats. <br />Materials and Methods: The animals were kept until 28 days after the bile duct ligation and received curcumin or curcumin nanomicelle via oral gavage at various doses during days 7-28. After the intervention, the effects of curcumin and curcumin nanomicelle on cardiovascular function, some inflammatory and antioxidant biomarkers, and histopathological changes were assessed. <br />Results: According to the findings, cardiac electrophysiology function and contractile force improved only in the curcumin nanomicelle groups. In addition, curcumin nanomicelle significantly reduced inflammatory factors and increased antioxidant enzymes. In the histopathological studies, cardiac tissue damage and destruction were observed to decrease in the curcumin nanomicelle groups. <br />Conclusion: Therefore, it was concluded that curcumin nanomicelle plays a protective role in cirrhotic cardiomyopathy by reducing inflammatory and oxidative factors and improving the cardiac function. Furthermore, curcumin nanomicelle exhibited more significant therapeutic effects compared to the curcumin treatment groups.
Cirrhosis,Cardiomyopathy,Curcumin,Nanomicelle,Inflammation
https://nmj.mums.ac.ir/article_15149.html
https://nmj.mums.ac.ir/article_15149_a753e6ba3a7c72890b755913c883fbaa.pdf