Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
A review on theranostic applications of iodine nanoparticles: Recent findings and perspectives
234
240
EN
Maryam
Mansouri
Department of Cell and Molecular Biology, Faculty of Chemistry, University of Kashan, Kashan, Iran
maryam.msor.77@gmail.com
Daryoush
Shahbazi-Gahrouei
0000-0002-8538-1898
Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
shahbazi@med.mui.ac.ir
10.22038/nmj.2021.56425.1575
Application of nanoparticles have in the core of researchers attention for both imaging and therapy of cancers. This review article aimed to prepare an outline on recent applications of iodine nanoparticles (INPs) as theranostic agents in both diagnosis and therapies. Among various strategies are used in treatment of cancers, radiotherapy with radiopharmaceutical agents especially radioisotope of iodine displays satisfactory results for numerous types of cancers. In recent years, new investigations were done in order to develop the novel structure of INPs. These nanoprobes could act as efficient theranostic purposes. Iodine nanoparticles may be applied in nuclear medicine imaging and may be effective with mega voltage (MV) photons in cancer therapy, but this remains to be tested with different cancer cells. By using INPs, effective steps can be taken in the future in both diagnosis and treatment of cancers. This review emphasized the recent research findings on the application of INPs in medical imaging and therapeutic of cancers. The current challenges and the perspectives for their future applications were also represented and discussed.
Iodine nanoparticles (INPs),Medical Imaging,Radiation Therapy,Theranostic
https://nmj.mums.ac.ir/article_18425.html
https://nmj.mums.ac.ir/article_18425_784181a1bd75028e4b5e8f9955ef3f0f.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
Smart terbinafine recent nano-advances in delivery of terbinafine
241
254
EN
Mohadesse
Mirshekari
Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran
m-mirshekari@gmail.com
Azar
Bagheri Ghomi
0000000266963024
Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran
azbagheri@gmail.com
Ahmad
Mehravaran
Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran
a-mehravaram@gmail.com
10.22038/nmj.2021.57263.1590
Terbinafine (TBF) is a drug with well-known antifungal properties effective against skin dermatophyte infections and nail particularly in treatment of pityriasis (tinea) vesicolor due to Malassezia furfur. Terbinafine topical administration is often recommended because commercial conventional terbinafine hydrochloride tablets are more expensive and have potential for significant adverse effects. Only less than 5% of terbinafine is absorbed in conventional topical forms. Novel nano-formulation approaches would be an efficient way to enhance penetration and abortion of topical drugs and eliminate limitations of conventional drug delivery systems. As conclusion, we believe that administering the Terbinafine in nano-formulations, according to different studies, could increases penetration of TBF through stratum corneum and viable epidermis and light the path of nano-structural delivery system in clinical application. Present overview aims to evaluate nano-strategies applied to improve permeation profile and terbinafine skin delivery. <br /><br />
Drug delivery, Nanoparticle,Percutaneous penetration,Terbinafine
https://nmj.mums.ac.ir/article_18419.html
https://nmj.mums.ac.ir/article_18419_3d62a824f00c2d463addf970b0718463.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
Synthesis of silver nanoparticles by Galega officinalis and its hypoglycemic effects in type 1 diabetic rats
255
263
EN
Fariba
Azimi
Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
azimi.f71314@gmail.com
Fariba
Mahmoudi
0000-0001-6092-1352
Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
f.mahmoudi@uma.ac.ir
Farzaneh
Mahmoudi
0000-0001-9046-8544
Department of chemistry, Shahid Beheshti University, G. C., Tehran, Iran
farzanehmahmoudi@yahoo.com
Mostafa
M.
Amini
0000-0003-2974-1024
Department of chemistry, Shahid Beheshti University, G. C., Tehran, Iran
m-pouramini@sbu.ac.ir
10.22038/nmj.2021.59391.1613
<em><strong>Objective(s):</strong></em> Diabetes is related with the higher blood levels of liver enzymes and inflammatory factors. Galega officinalis is used as a medicinal plant for treatment of diabetes traditionally. In this work, silver nanoparticles (Ag-NPs) were synthesized with green method using Galega officinalis extract.<br /><em><strong>Materials and Methods: </strong></em>The synthesized green Ag-NPs were characterized completely. Intact or diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg green synthesized Ag-NPs. Mean serum levels of glucose, hepatic enzymes and hematological parameter were determined. Gene expression of tumor necrotic factor alpha (TNF-α) was done by real-time PCR. <br /><em><strong>Results:</strong></em> Synthesis of green synthesized Ag-NPs was confirmed by FT-IR, XRD and UV-vis analyses. The FESEM and TEM images showed spherical Ag-NPs with size of 25 nm. The hypoglycemic influence of Ag-NPs using Galega officinalis extract is reported for the first time in this study. Blood concentration of liver enzymes, urea, glucose, white blood cells count and TNF-α mRNA levels in visceral adipose tissue significantly declined in diabetic rats receiving Ag-NPs.<br /><em><strong>Conclusion:</strong></em> The synthesized Ag-NPs using Galega officinalis extract may improve complication of diabetes via preventing liver hepatocyte damage and reducing inflammatory factors.
Diabetes,Galega officinalis,Liver enzymes,Silver nanoparticle,TNF-α
https://nmj.mums.ac.ir/article_18848.html
https://nmj.mums.ac.ir/article_18848_bd0a94b80d3a4d7fd1bf2acf4abecee6.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
Synthesis of L-DOPA conjugated doxorubicin-polyethylenimine nanocarrier and evaluation of its cytotoxicity on A375 and HepG2 cell lines
264
269
EN
Kimia
Mansouri
Pharmaceutical Sciences Research center, Shiraz University of Medical Sciences, Shiraz, Iran
mansourikimia1996@gmail.com
Fatemeh
Ahmadi
Pharmaceutical Sciences Research center, Shiraz University of Medical Sciences, Shiraz, Iran
ahmadi_f@sums.ac.ir
Ali
Dehshahri
0000-0003-4458-1292
Pharmaceutical Sciences Research center, Shiraz University of Medical Sciences, Shiraz, Iran
dehshahria@sums.ac.ir
10.22038/nmj.2021.59681.1615
<em><strong>Objective(s):</strong></em> Polyethylenimine (PEI) is one of the most-extensively investigated cationic polymers for gene and drug delivery. Recently, great attention has been directed to design of carriers for co-delivery of nucleic acids and small molecules. These delivery systems are able to overcome the limitations of gene or drug delivery alone. The aim of this study is to prepare a targeted nano-carrier for co-delivery of doxorubicin (Dox) and gene using polyethylenimine. <br /><em><strong>Materials and Methods:</strong></em> In order to prepare the ligand-containing polymer conjugates, succinic anhydride was conjugated onto the hydroxyl group of Dox through an ester bond following the protection of Dox amines by Fmoc. Drug-polymer conjugates were then coupled with L-DOPA in order to prepare the targeted nanocarriers to the cells through Large Amino Acid Transporter-1 (LAT-1). The PEI derivatives were characterized using 1H-NMR. The toxicity of conjugated polymer, Dox and PEI was assessed on HepG2 and A375 cell lines with different expression level of LAT-1 transporters using MTT assay. <br /><em><strong>Results:</strong> </em>The chemical structure of PEI conjugate was confirmed by 1H-NMR. The cytotoxicity measurement demonstrated a cell line-dependent toxicity profile at the concentrations tested in this study. It was shown that there was no significant difference in cell-induced toxicity between conjugated polymer and its parent form in A375 cell line while the cytotoxicity of conjugated polymer was significantly lower than the parent PEI in HepG2 cells.<br /><em><strong>Conclusion:</strong></em> These results provide promising evidence for further evaluation of PEI conjugate for co-delivery of drug and gene via LAT-1 transporters.
Cytotoxicity,Doxorubicin,L-DOPA,Polyethylenimine
https://nmj.mums.ac.ir/article_18619.html
https://nmj.mums.ac.ir/article_18619_5825be5f822413688614b92f28615dc1.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
Evaluation of mPEG-PLA nanoparticles as vaccine delivery system for modified protective antigen of Bacillus anthracis
270
278
EN
Seyed Masih
Etemad aubi
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
Hosein
Honari
0000-0003-9116-3104
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
honari.hosein@gmail.com
Hamed
Bagheri
Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, 14115-336, Iran
Rohollah
Ghasemi
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, 14115-175, Iran
Mojtaba
Noofeli
Department of Human Bacterial Vaccines Production & Research, Razi Vaccine and Serum Research Institute, Karaj, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
Seyed Mojtaba
Aghaie
0000-0003-0201-9592
Department of Biology, Faculty of Basic Science, Imam Hossein University, Tehran, Iran
mojtabaasefi@yahoo.com
10.22038/nmj.2021.59843.1616
<em><strong>Objective(s):</strong></em> Bacillus anthracis is the cause of the fatal anthrax. Available anthrax vaccines have low stability and require multiple injections in order to be effective. Poly lactic acid (PLA) has been approved as a biodegradable and biocompatible polymer for drug and vaccine delivery applications. The purpose of this study is to evaluate the antibody titer against the protective antigen recombinant protein (PA63) encapsulated by the mPEG-PLA double-block copolymers and to compare with the non-encapsulated PA63.<br /><em><strong>Materials and Methods:</strong></em> To attain this purpose, to start, the desired protein was purified and confirmed and then PA63 was encapsulated with mPEG-PLA double-block copolymers using a water- oil- water solvent evaporation method. Produced nanoparticles was characterized in terms of morphological specifications using scanning electron microscopy, size and polydispersity index using dynamic light scattering and zeta potential using a zeta seizer. The synthesized nanoparticle antigenic content and also its antigen release profile was measured. In the following, the nanoparticles containing antigens (PA63-NPs), blank nanoparticles (mPEG-PLA- NPs), PA63 and adjuvant control were injected subcutaneously to mice and the IgG polyclonal antibody titr was measured by indirect ELISA. Finally to evaluate biocompatibility and toxicity, synthesized nanoparticles were investigated by cell culture testing.<br /><em><strong>Results:</strong></em> The results of this study showed that the synthesized nanoparticles are of good quality. ELISA results showed that antibody production titr in mice receiving PA63-NPs was higher than those receiving the PA63 (P<0.05). Cell culture results revealed that the synthesized nanoparticles have no toxicity.<br /><em><strong>Conclusion:</strong></em> The findings of the study indicated that the obtained nano vaccine formulations had a higher ability than non-encapsulated recombinant proteins to stimulate the immune system of animal, and that PLA could be used as an appropriate carrier for an effective, stable, safe and biodegradable engineered recombinant vaccine against anthrax.
Anthrax,Protective antigen,Vaccine,mPEG-PLA
https://nmj.mums.ac.ir/article_18867.html
https://nmj.mums.ac.ir/article_18867_53c0ab5c33f399b321bba16a4af5280a.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
Synthesis and evaluation of SPION@CMD@Ser-LTVSPWY peptide as a targeted probe for detection of HER2+ cancer cells in MRI
279
289
EN
Arash
Papi
0000-0001-9155-046X
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
arash.papi4492@gmail.com
Rasoul
Irajirad
Finetech in Medicine Research Center, Iran University of Medical Sciences, Tehran, Iran
irajirad.r@gmail.com
Milad
Yousefvand
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
mi.yousefvand@gmail.com
Ali Reza
Montazerabadi
Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
alireza.montazerabadi@gmail.com
Zahra
Mohammadi
Radiological technology department of actually paramedical sciences, Babol University of medical sciences
mehr_055@yahoo.com
10.22038/nmj.2021.59629.1614
<em><strong>Objective(s):</strong></em> Successful detection of tumors in the early stages can significantly increase a patient’s healing process and recovery speed. Conventional imaging techniques usually depend on the tissues’ anatomical structure. Epidermal growth factor receptor-2 (HER-2) is a transmembrane protein with an extracellular ligand-binding domain. HER2 plays an essential role in cell proliferation, differentiation, and survival, and its overexpression is associated with various cancers, especially breast and ovarian cancers. Access to its extracellular domain makes HER2 an ideal target for drug preparation and molecular imaging probes. In this study, a targeted magnetic nanoprobe for molecular imaging of HER2 positive cancers was synthesized, and also its potential as a T2-weighted targeted contrast agent was assessed.<br /><em><strong>Materials and Methods:</strong></em> Superparamagnetic SPION nanoparticles were synthesized using the co-precipitation method in the presence of CMD and were labeled with SLTVSPWY peptide. The SPION@CMD@SLTVSPWY nanocomplex was characterized by TEM, DLS, XRD, AAS, FTIR, EDX, and VSM. The r1 and r2 relaxivities were then calculated using a 1.5 Tesla clinical magnetic field. The cytotoxicity of the nanocomplex was evaluated by MTT assay. Finally, the difference between uptake of targeted nanocomplexes and SPION by the human SKOV-3 cell line (HER2 +) was investigated.<br /><em><strong>Results:</strong></em> The SPION@CMD NPs were synthesized with spherical shape and superparamagnetic behavior. Characterization results confirmed the formation of SPION@CMD@SLTVSPWY. r2 relaxivity and r2/r1 calculations resulted in suitable values of 313 mM-1s-1 and 8.05 for SPION@CMD@SLTVSPWY, respectively. Increased uptake of targeted nanocomplexed (SPION@CMD@SLTVSPWY) compared to non-targeted NPs (SPION@CMD) was very noticeable visually, and its numerical ratio was 3.51 at a concentration of 0.075 mM. <br /><em><strong>Conclusion:</strong></em> The targeted synthesized nanocomplex in this study has great potential as a T2 weighted probe contrast agent in MR imaging owing to its appropriate high uptake in HER2 + cells.
Cancer,HER2 Receptor,LTVSPWY peptide,MRI,SKOV-3,SPION
https://nmj.mums.ac.ir/article_18689.html
https://nmj.mums.ac.ir/article_18689_4338435e63018a8c5b7966e8a4fd5e20.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
Anti-tumor activity of nanoliposomes containing crude extract of saffron in mice bearing C26 colon carcinoma
290
297
EN
Sanaz
Abbaszadegan
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
sabbaszadegan@gmail.com
Hossein
Hosseinzadeh
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
hosseinzadehh@mums.ac.ir
Seyedeh Hoda
Alavizadeh
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
alavizadehh@mums.ac.ir
Marziyeh
Moghri
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
alavizadehh@gmail.com
Azam
Abbasi
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
abbasia2@mums.ac.ir
Mahmoud Reza
Jaafari
0000-0003-3908-6828
Nanotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
jafarimr@mums.ac.ir
10.22038/nmj.2021.60221.1623
<em><strong>Objective(s):</strong></em> Saffron, the dehydrated stigma of the Crocus sativus L. flower, has been reputed as an effective anticancer and chemopreventive agent in cancer therapy. This study aimed to design PEGylated nanoliposomes containing crude extract of saffron for the treatment of cancer.<br /><em><strong>Materials and Methods:</strong></em> Various PEGylated nanoliposomes containing 25 mg/ml aqueous extract of saffron were prepared using the thin lipid film method. The characterization of liposomes was indicated by their size, in vitro cytotoxicity, and in vivo therapeutic efficacy against C26 tumor-bearing mice. <br /><em><strong>Results:</strong></em> By increasing cholesterol levels, the IC50 values of the formulations increased. Liposome characterization illustrated the properties of formulation of choice, as follows: Z-average size: 73.7 ± 1.3 nm; PDI: 0.103 ± 0.035; zeta potential: -20.8 mV ± 3.7; % encapsulation: 91 ± 0.059, % release after 168 hours in 30% FBS: 16.26 ± 0.01.<br /><em><strong>Conclusion: </strong></em>Treating tumor-bearing mice with the selected saffron liposomes indicated that, for the first time, the i.v. injection of nano-liposomal saffron at a dose of 300 mg/kg significantly increased the anti-tumor property compared to the negative control group, while no significant difference was observed compared with aqueous extract of saffron. Hence, to achieve an optimal formulation for human use, the formulation merits further study.
Anti-tumor activity,C26 colon carcinoma,Experimental study,PEGylated nanoliposome,Saffron
https://nmj.mums.ac.ir/article_18866.html
https://nmj.mums.ac.ir/article_18866_b06e7b8f2447829c244775343406e0af.pdf
Mashhad University of Medical Sciences
Nanomedicine Journal
2322-3049
2322-5904
8
4
2021
10
01
Gadoterate meglumine - anionic linear globular dendrimer second generation: A novel nano sized theranostic contrast agent
298
306
EN
Noorin
Yousefiyeh
0000-0001-9810-9773
Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
noorinyousefiyeh@gmail.com
Abolfazl
Arab
0000-0002-0024-2937
Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
abolfazl.arab1996@gmail.com
Elnaz
Salehian
0000-0003-4977-697X
Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
salehian.elnaz1994@gmail.com
Radin
Alikhani
Department of Medicinal Chemistry, School of Pharmacy, Ardabil University of Medical Sciences, Ardabil, Iran
radinalikhani.ra@gmail.com
Shabnam
Samimi
0000-0002-6984-3933
Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
shabnam.samimi@yahoo.com
Artin
Assadi
0000-0001-7255-2267
Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
assadi.artin@gmail.com
Mehdi
Shafiee Ardestani
https://orcid.org/00
Department of Radiopharmacy, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
shafieeardestani@gmail.com
10.22038/nmj.2021.58980.1607
<em><strong>Objective(s):</strong> </em>Cancer is known as one of the most life-threatening diseases in the world. Early diagnosis of cancer may significantly increase the chance of effective treatment. In the recent years, the importance of medical imaging usage has been increased to identify cancer’s nature and pattern of growth in order to provide the most advantageous treatment approaches for cancer tumors. Magnetic resonance imaging is an efficient non-invasive tool for early diagnosis of cancer which provides clear scans of various tissues without radiation. Contrast Agents such as Gadoterate Meglumine enhance contrast MR imaging and provide imaging from inside the cells without entering them.<br /><em><strong>Materials and Methods:</strong></em> In this study, Gadoterate Meglumine nano-sized anionic linear globular dendrimer second generation was first synthesized and then qualitative and quantitative methods were carried out to ensure the proper synthesis and to assess the toxicity of the compound. Once the non-toxicity of the chemical was ensured, in vivo MR imaging studies was performed to test the impact of the synthesized compound on the resolution of image. <br /><em><strong>Results:</strong></em> The result obtained from this study demonstrated that the attachment of Gadolinium (III) to a nano dendrimer reduces its cytotoxicity and also improved resolution of image. In this research, Gadoterate Meglumine nano-sized anionic linear globular dendrimer second generation was effectively able to enter the cells while showing low cytotoxicity in the normal cells and moderate cytotoxicity on cancer cells. <br /><em><strong>Conclusion:</strong></em> Therefore, ALGDG2-GM could be introduced as a novel, safe, effective and promising nano-sized theranostic contrast agent candidate.<br /><br />
Cancer, Diagnostic,MRI, Nanoparticle, Therapeutic
https://nmj.mums.ac.ir/article_18719.html
https://nmj.mums.ac.ir/article_18719_e9f4b4dc92d5f56e5559391f8e46b7ba.pdf