TY - JOUR ID - 19819 TI - Aptamer AS1411-functionalized gold nanoparticle-melittin complex for targeting MCF-7 breast cancer cell line JO - Nanomedicine Journal JA - NMJ LA - en SN - 2322-3049 AU - Bayat, Payam AU - Abnous, Khalil AU - Balarastaghi, Soudabeh AU - Taghdisi, Seyed Mohamaad AU - Saeedi, Majid AU - Yazdian-Robati, Reavan AU - Mahmoudi, Mahmoud AD - Immunology Research Center, BuAli Research Institute, Department of Immunology and Allergy, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran AD - Department of Pharmaceutics, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran AD - Molecular and Cell Biology Research Center, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran Y1 - 2022 PY - 2022 VL - 9 IS - 2 SP - 164 EP - 169 KW - AS1411aptamer KW - Gold Nanoparticle KW - Melittin KW - Nucleolin KW - Targeted therapy DO - 10.22038/nmj.2022.62476.1649 N2 - Objective(s): Several studies reported the apoptotic and lytic activity of melittin (Mel) in different tumor cells. In this study, a novel nano-complex was developed composed of AS1411aptamers, melittin and gold nanoparticle for the treatment of breast cancer cells. Materials and Methods: Gold nanoparticles (GNP) were synthesized using reduction of tetrachloroauric acid (HAuCl4). Melittin modified with cysteine and AS1411aptamer conjugated to the gold nanoparticle. Gel retardation assay was used to prove the formation of GNP-Mel-AS1411 complex. Physicochemical properties of complex were investigated by Dynamic Light Scattering (DLS). The cytotoxicity of Mel and GNP-Mel-AS1411 complex were evaluated in both MCF‐7 (target) and L929 (non-target) cells by the MTT assay. Results: The average size of GNP and GNP-Mel-AS1411 complex were 20 ± 2.5 and 270.5± 3.2 respectively. The results of MTT assay revealed that this nanocomplex was more cytotoxic in MCF‐7 (cell viability = 19% ± 2%) and less cytotoxic in L929 cells (cell viability = 73% ± 1.6%).Conclusion: The results of this study indicated that the gold nanoparticle-melittin-AS1411 complex had a potential value in cancer cell targeted delivery of melittin.  UR - https://nmj.mums.ac.ir/article_19819.html L1 - https://nmj.mums.ac.ir/article_19819_ec7a1222162cc56e305e5e1040b6ff9a.pdf ER -