Green synthesis and antibacterial activity of zinc selenide (ZnSe) nanoparticles

Document Type: Research Paper

Authors

1 Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Biotechnology, Higher Education Institute of Rabe-Rashid, Tabriz, Iran

3 Liver and Digestive Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran; Department of Microbiology, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran

4 Department of Biotechnology, Higher Education Institute of Rabe-Rashid, Tabriz, Iran; Department of Biotechnology, East Azerbaijan Agricultural Education Center, Tabriz, Iran

Abstract

Objective(s): Drug delivery is an engineering technology to control the release and delivery of therapeutic agents to target organs, tissues, and cells. Metallic nanoparticles, such as gold nanoparticles (AuNPs) have exceptional properties which enable efficient drug transport into different cell types with reduced side effects and cytotoxicity to other tissues.
Materials and Methods: AuNPs were synthesized by adopting the Turkevich method to reduce tetra chloroauric (III) acid (HAuCl4) solution with sodium citrate. A factorial design of 24 was used to investigate the influence of temperature, stirring speed, and the volume of citrate and gold salt on the size of AuNPs synthesis. The produced chemical-AuNPs (CN-AuNPs) were characterized using ultraviolet-visible spectroscopy and dynamic light scattering (DLS) which was conjugated with polyethylene glycol (PEG) loaded with chloroquine diphosphate. The latter were characterized with transmission electron microscopy (TEM), Energy dispersive x-ray spectroscopy (EDS), selected area electron diffraction (SAED) patterns and Fourier transmission infrared spectroscopy. The antimalarial activities of the three formulations were tested on Plasmodium-infected mice. Moreover, the evaluation of curative potentials of the formulations was carried out via parasite counts. The anemic and pathological conditions of nano-encapsulation were investigated for their cytotoxicity level.
Results: The CN-AuNPs show surface plasmon resonance absorption ranging from 526 to 529 nm with smaller particle size at the lower citrate volume. The TEM image of CN-AuNPs with polyethylene glycol (PEG) and CN-AuNPs-PEG encapsulated with chloroquine diphosphate revealed spherical shape with EDS showing the appearance of gold (Au) at 2.0, 2.1, and 9.9 KeV. The SAED also revealed that the AuNPs were crystalline in nature. The in vitro time-dependent encapsulation release showed an extension of time release, compared to CN-AuNPs-PEG with parasitemia clearance at the same level of cytotoxicity.
Conclusion: Therefore, although improved activity of the CN-AuNPs-PEG encapsulating was achieved but its cytotoxicity still is a limitation.

Keywords


1.Sirelkhatim A, Mahmud S, Seeni A, Kaus N, Ann L, Bakhori S, et al. Review on Zinc Oxide Nanoparticles: Antibacterial Activity and Toxicity Mechanism. Nano-Micro Lett. 2015; 7(3): 219-242.
2.De M, Ghosh PS, Rotello VM. Applications of Nanoparticles in Biology. Adv Mater. 2008; 20(22): 4225-4241.
3.Vidic J, Stankic S, Haque F, Ciric D, Le Goffic R, Vidy A. Selective antibacterial effects of mixed ZnMgO nanoparticles. J Nanoparticle Res. 2013; 15(5): 1595-1605.
4.Rasmussen JW, Martinez E, Louka P, Wingett DG. Zinc Oxide Nanoparticles for Selective Destruction of Tumor Cells and Potential for Drug Delivery Applications. Expert Opin Drug Deliv. 2010; 7(9): 1063-1077.
5.Yadav K, Giri M, Jaggi N. Synthesis, characterization and photocatalytic studies of ZnSe and Ag:ZnSe nanoparticles. Res Chem Intermediate. 2015; 41(12): 9967-9978.
6.Darroudi M, Hakimi M, Goodarzi E, Kazemi Oskuee R. Superparamagnetic iron oxide nanoparticles (SPIONs): Green preparation, characterization and their cytotoxicity effects. Ceram Int. 2014; 40(9, Part B): 14641-14645.
7.Darroudi M, Sabouri Z, Kazemi Oskuee R, Khorsand Zak A, Kargar H, Abd Hamid MHN. Green chemistry approach for the synthesis of ZnO nanopowders and their cytotoxic effects. Ceram Int. 2014; 40(3): 4827-4831.
8.Zak AK, Hashim AM, Darroudi M. Optical properties of ZnO/BaCO3 nanocomposites in UV and visible regions. Nanoscale Res Lett. 2014; 9(1): 1-6.
9.Zamiri R, Azmi BZ, Darroudi M, Sadrolhosseini A, Husin MS, Zaidan AW. Preparation of starch stabilized silver nanoparticles with spatial self-phase modulation properties by laser ablation technique. Appl Phys A. 2011; 102(1): 189-194.
10.Zamiri R, Zakaria A, Ahmad MB, Sadrolhosseini AR, Shameli K, Darroudi M. Investigation of spatial self-phase modulation of silver nanoparticles in clay suspension. Optik. 2011; 122(9): 836-838.
11.Peeters E, Nelis HJ, Coenye T. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Meth. 2008; 72(2): 157-165.
12.Van den Driessche F, Rigole P, Brackman G, Coenye T. Optimization of resazurin-based viability staining for quantification of microbial biofilms. J Microbiol Meth. 2014; 98: 31-34.
13.Archana J, Navaneethan M, Prakash T, Ponnusamy S, Muthamizhchelvan C, Hayakawa Y. Chemical synthesis and functional properties of magnesium doped ZnSe nanoparticles. Mater Lett. 2013; 100: 54-57.
14.Li Y, Ding Y, Qian Y, Zhang Y, Yang L. A Solvothermal Elemental Reaction to Produce Nanocrystalline ZnSe. Inorg Chem. 1998; 37(12): 2844-2845.
15.Liang Q, Bai Y, Han L, Deng X, Wu X, Wang Z. Hydrothermal synthesis of ZnSe:Cu quantum dots and their luminescent mechanism study by first-principles. J Lumin. 2013; 143: 185-192.
16.Khataee AR, Hosseini M, Hanifehpour Y, Safarpour M, Joo SW. Hydrothermal synthesis and characterization of Nd-doped ZnSe nanoparticles with enhanced visible light photocatalytic activity. Res Chem Intermediate. 2014; 40(2): 495-508.
17.Park H, Chung H, Kim W. Synthesis of ultrathin wurtzite ZnSe nanosheets. Mater Lett. 2013; 99: 172-175.
18.Molaei M, Khezripour AR, Karimipour M. Synthesis of ZnSe nanocrystals (NCs) using a rapid microwave irradiation method and investigation of the effect of copper (Cu) doping on the optical properties. Appl Surf Sci. 2014; 317: 236-240.
19.Qin H, Jian W, Zhang Y, Kim T, Jiang Z, Jiang D. A simple and novel route for the synthesis of water soluble ZnSe quantum dots using the Nano-Se as the reaction intermediate. Mater Lett. 2012; 67(1): 28-31.
20.Khataee A, Arefi-Oskoui S, Abdollahi B, Hanifehpour Y, Joo S. Synthesis and characterization of Pr x Zn1−x Se nanoparticles for photocatalysis of four textile dyes with different molecular structures. Res Chem Intermediate. 2015; 41(11): 8425-8439.
21.Hsieh SH, Chen WJ, Yeh TH. Degradation of methylene blue using ZnSe–graphene nanocomposites under visible-light irradiation. Ceram Int. 2015; 41(10, Part A): 13759-13766.
22.Khandan Nasab N, Dehnad AR, Salimizand H, Taherzadeh D, Prakash D, Verma K. Zinc selenide nanoparticles (ZnSe-NPs): Green synthesis and investigation of their cytotoxicity effects. Ceram Int. 2016; 42(10): 12115-12118.
23.O’Toole GA. Microtiter Dish Biofilm Formation Assay. J Vis Exp. 2011; (47): 2437-2438.