An artificial blood vessel fabricated by 3D printing for pharmaceutical application

Document Type : Research Paper

Authors

1 New Technologies Research Center, Amirkabir University of Technology, Tehran, 15875-4413, Iran

2 Department of Quality Control, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran

3 Department of Pharmacy, Eastern Mediterranean University, Gazimagusa, TRNC, via Mersin 10, Turkey

4 Students Research Committee, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 Department of Urology, AJA University of Medical Sciences, Tehran, Iran

6 Department of Industrial Engineering, Eastern Mediterranean University, Gazimagusa, TRNC, Via Mersin 10, Turkey

Abstract

Objective(s): Cardiovascular diseases (CVDs) are the leading cause of mortality in the elderly. A common medical procedure for the treatment of CVDs is the replacement of the blocked or narrowed arteries, which is currently the optimal vascular transplant associated with autograft transplantation. In general, the saphenous veins and radial arteries in the mammary gland are considered to be the selective vessels for vascular substitution. In many cardiac patients, artificial blood vessels (ABVs) are not used for several reasons, including the age of the patient, small size of the veins, previous impressions, and abnormally. Therefore, the consideration of vascular substitute demands is inevitable, especially regarding vascular transplantation with very small diameters and availability of proper alternatives. The present study aimed to develop a novel artificial bio-composite blood vessel using polymer-reinforced and bioceramic nanoparticles.
Materials and Methods: The biomechanics and chemical properties of artificial vessels have been investigated to be used in coronary artery bypassing in atherosclerosis as a soft tissue engineering procedure. In this study, thermoplastic polyurethane (TPU) composed of nanocrystalline hydroxyapatite (HA) nanopowder was prepared using the extrusion technique to construct the ABVs. X-ray diffraction (XRD) and scanning electron microscopy (SEM) were used to investigate the optimum specimen. An important feature of the ABVs was the ability to find the elastic modulus, wettability, and porosity of the veins, which were assessed by fused deposition modeling and 3D printing.
Results: The sample containing five wt% of HA had superior mechanical and biological features over the pure sample.
Conclusion: According to the results, the narrowed arteries composed of TPU composite with nanocrystalline HA nanopowder had proper chemical stability and mechanical characteristics.

Keywords


1.Yancopoulos GD, Davis S, Gale NW, Rudge JS, Wiegand SJ, Holash J. Vascular-specific growth factors and blood vessel formation. Nature. 2000; 407(6801): 242.
2.Ahmed M, Hamilton G, Seifalian AM. The performance of a small-calibre graft for vascular reconstructions in a senescent sheep model. Biomat. 2014; 35(33): 9033-90340.
3.Jiang YC, Jiang L, Huang A, Wang XF, Li Q, Turng LS. Electrospun polycaprolactone/gelatin composites with enhanced cell–matrix interactions as blood vessel endothelial layer scaffolds. Mater Sci Eng C Mater Biol Appl. 2017; 71: 901-908.
4.Mostafavi F, Golshan Ebrahimi N. Physical characterization and rheological behavior of polyurethane/poly (caprolactone) blends, prepared by solution blending using dimethylacetamide. J Appl Polym Sci. 2012; 125(5): 4091-4099.
5.Kapadia MR, Popowich DA, Kibbe MR. Modified prosthetic vascular conduits. Circulation. 2008; 117(14): 1873-1882.
6.Lutolf MP, Hubbell JA. Synthetic biomaterials as instructive extracellular microenvironments for morphogenesis in tissue engineering. Nature Biotechnol. 2005; 23(1): 47.
7.Vaz C, M Van, Tuijl S, Bouten C V C, Baaijens F P T. (2005). Design of scaffolds for blood vessel tissue engineering using a multi-layering electrospinning technique. Acta Biomater. 1(5), 575-582.
8.Griffith LG, Naughton G. Tissue engineering current challenges and expanding opportunities. Science. 2002; 295(5557): 1009-10014.
9.Gao F, Watanabe M, Matsuzawa T. Stress analysis in a layered aortic arch model under pulsatile blood flow. Biomed Eng Online. 2006; 5(1):25.
10.Harrison JH. Synthetic materials as vascular prostheses: II. A comparative study of nylon, dacron, orlon, ivalon sponge and teflon in large blood vessels with tensile strength studies. Am J Surg. 1958; 95(1): 16-24.
11.Zhou M, Wang WC, Liao YG, Liu WQ, Yu M, Ouyang CX. In vitro biocompatibility evaluation of silk-fibroin/polyurethane membrane with cultivation of HUVECs. Front Mater Sci. 2014; 8(1): 63-71.
12.Heydary HA, Karamian E, Poorazizi E, Heydaripour J, Khandan A. Electrospun of polymer/bioceramic nanocomposite as a new soft tissue for biomedical applications. J Asian Ceram Soc. 2015 ;3(4): 417-425.
13.Heydary HA, Karamian E, Poorazizi E, Khandan A, Heydaripour J. A novel nano-fiber of Iranian gum tragacanth-polyvinyl alcohol/nanoclay composite for wound healing applications. Proc Mater Sci. 2015; 11: 176-182.
14.Salami MA, Kaveian F, Rafienia M, Saber-Samandari S, Khandan A, Naeimi M. Electrospun polycaprolactone/lignin-based nanocomposite as a novel tissue scaffold for biomedical applications. Med Signals Sens. 2017; 7(4): 22.
15.Khandan A, Jazayeri H, Fahmy MD, Razavi M. Hydrogels: types, structure, properties, and applications. Biomat Tiss Eng. 2017; 4: 143-169.
16.Razavi M, Khandan A. Safety, regulatory issues, long-term biotoxicity, and the processing environment. In Nanobiomat. Sci Dev Eval. 2017(pp. 261-279). Woodhead Publishing.
17.Kordjamshidi A, Saber-Samandari S, Nejad MG, Khandan A. Preparation of novel porous calcium silicate scaffold loaded by celecoxib drug using freeze drying technique: Fabrication, characterization and simulation. Ceram Int. 2019.
18.Saber‐Samandari S, Afaghi‐Khatibi A. Evaluation of elastic modulus of polymer matrix nanocomposites. Polym Compos. 2007; 28(3): 405-411.
19.Saber-Samandari S, Saber-Samandari S, Kiyazar S, Aghazadeh J, Sadeghi A. In vitro evaluation for apatite-forming ability of cellulose-based nanocomposite scaffolds for bone tissue engineering. Int J Biol Macromol. 2016; 86: 434-442.
20.Martin DJ, Warren LA, Gunatillake PA, McCarthy SJ, Meijs GF, Schindhelm K. Polydimethylsiloxane/polyether-mixed macrodiol-based polyurethane elastomers: biostability. Biomat. 2000; 21(10): 1021-1029.
21.Kidane AG, Burriesci G, Edirisinghe M, Ghanbari H, Bonhoeffer P, Seifalian AM. A novel nanocomposite polymer for development of synthetic heart valve leaflets. Acta Biomater. 2009; 5(7): 2409-2417.
22.Bonesi M, Kennerley AJ, Meglinski I, Matcher S. Application of Doppler optical coherence tomography in rheological studies: blood flow and vessels mechanical properties evaluation. J Innov Opt Health Sci. 2009; 2(4): 431–440.
23.Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomater. 2006; 27(15): 2907-2915.
24.Chlupáč J, Filova E, Bačáková L. Blood vessel replacement: 50 years of development and tissue engineering paradigms in vascular surgery. Physiol Res. 2009; 58(Suppl 2): S119-139.
25.Seifu DG, Purnama A, Mequanint K, Mantovani D. Small-diameter vascular tissue engineering. Nat Rev Cardiol. 2013; 10(7): 410.
26.Puskas JE, Chen Y. Biomedical application of commercial polymers and novel polyisobutylene-based thermoplastic elastomers for soft tissue replacement. Biomacromolecules. 2004; 5(4): 1141-1154.
27.Major R, Sanak M, Lackner JM, Bruckert F, Marczak J, Major B. Bioinspired thin film materials designed for blood contact. In Hemocomp of Biomat. for Clin. Appl. 2018 pp. 327-356. Woodhead Publishing.
28.Liu L, Wang X. Creation of a vascular system for organ manufacturing. Int J Bioprint. 2015; 1(1).
29.Hu X, Hu T, Guan G, Yu S, Wu Y, Wang L. Control of weft yarn or density improves biocompatibility of PET small diameter artificial blood vessels. J Biomed Mater Re. B. 2018; 106(3): 954-964.
30.Harper AM. Autoregulation of cerebral blood flow: influence of the arterial blood pressure on the blood flow through the cerebral cortex. J Neurol Neurosurg Psychiatry. 1966; 29(5): 398.
31.Kim BS, Park IK, Hoshiba T, Jiang HL, Choi YJ, Akaike T, Cho CS. Design of artificial extracellular matrices for tissue engineering. Prog Polym Sci. 2011; 36(2): 238-268.
32.Gunatillake PA, Adhikari R. Biodegradable synthetic polymers for tissue engineering. Eur Cell Mater. 2003; 5(1): 1-6.
33.Gao W, Wang J. Synthetic micro/nanomotors in drug delivery. Nanoscale. 2014; 6(18): 10486-10494.
34.Goodman SL, Sims PA, Albrecht RM. Three-dimensional extracellular matrix textured biomaterials. Biomater. 1996; 17(21): 2087-2095.
35.Fang J, Nakamura H, Maeda H. The EPR effect: unique features of tumor blood vessels for drug delivery, factors involved, and limitations and augmentation of the effect. Adv Drug Deliv Rev. 2011; 63(3): 136-151.
36.andeniyage LS, Gunatillake PA, Adhikari R, Bown M, Shanks R, Adhikari B. Development of high strength siloxane poly (urethane‐urea) elastomers based on linked macrodiols for heart valve application. J Biomed Mater Res B. 2018; 106(5): 1712-17120.
37.Dandeniyage LS, Adhikari R, Bown M, Shanks R, Adhikari B, Easton CD, Gengenbach TR, Cookson D, Gunatillake PA. Morphology and surface properties of high strength siloxane poly (urethane‐urea) s developed for heart valve application. J Biomed Mater Res B Appl Biomater. 2019; 107(1): 112-121.
38.Griffin M, Naderi N, Kalaskar DM, Malins E, Becer R, Thornton CA, Whitaker IS, Mosahebi A, Butler PE, Seifalian AM. Evaluation of sterilisation techniques for regenerative medicine scaffolds fabricated with polyurethane nonbiodegradable and bioabsorbable nanocomposite materials. Int J Biomater. 2018; 2018.
39.Lewis AL, Tolhurst LA, Stratford PW. Analysis of a phosphorylcholine-based polymer coating on a coronary stent pre-and post-implantation. Biomater. 2002; 23(7): 1697-1706.
40.Klement P, Du YJ, Berry LR, Tressel P, Chan AK. Chronic performance of polyurethane catheters covalently coated with ATH complex: a rabbit jugular vein model. Biomater. 2006; 27(29): 5107-5117.
41.Campoccia D, Montanaro L, Arciola CR. A review of the biomaterials technologies for infection-resistant surfaces. Biomater. 2013; 34(34): 8533-8554.
42.Mohanty M, Kumary TV, Lal AV, Sivakumar R. Short term tissue response to carbon fibre: A preliminaryin vitro and in vivo study. Bull Mater Sci. 1998; 21(6): 439-444.
43.Ghadirinejad M, Atasoylu E, Izbirak G, Ghasemi M. A Stochastic Model for the Ethanol Pharmacokinetics. Iran J Public Health. 2016; 45(9): 1170.
44.Lee SJ, Heo DN, Park JS, Kwon SK, Lee JH, Lee JH, Kim WD, Kwon IK, Park SA. Characterization and preparation of bio-tubular scaffolds for fabricating artificial vascular grafts by combining electrospinning and a 3D printing system. Phys Chem Chem Phys. 2015; 17(5): 2996-2999.
45.Khandan A, Ozada N, Saber-Samandari S, Nejad MG. On the mechanical and biological properties of bredigite-magnetite (Ca7MgSi4O16-Fe3O4) nanocomposite scaffolds. Ceram Int. 2018; 44(3): 3141-3148.
46.Ghayour H, Abdellahi M, Nejad MG, Khandan A, Saber-Samandari S. Study of the effect of the Zn 2+ content on the anisotropy and specific absorption rate of the cobalt ferrite: the application of Co1−xZnxFe2O4 ferrite for magnetic hyperthermia. J Aust Ceram Soc. 2018; 54(2): 223-230.
47.Sahmani S, Shahali M, Nejad MG, Khandan A, Aghdam MM, Saber-Samandari S. Effect of copper oxide nanoparticles on electrical conductivity and cell viability of calcium phosphate scaffolds with improved mechanical strength for bone tissue engineering. EPJ Plus. 2019; 134(1): 7.
48.Sahmani S, Khandan A, Saber-Samandari S, Aghdam MM. Vibrations of beam-type implants made of 3D printed bredigite-magnetite bio-nanocomposite scaffolds under axial compression: Application, communication and simulation. Ceram Int. 2018; 44(10): 11282-11291.
49.Moradi-Dastjerdi R, Aghadavoudi F. Static analysis of functionally graded nanocomposite sandwich plates reinforced by defected CNT. Compos Struct. 2018;200: 839-848.
50.Aghadavoudi F, Golestanian H, Tadi Beni Y. Investigating the effects of resin crosslinking ratio on mechanical properties of epoxy‐based nanocomposites using molecular dynamics. Poly Compos. 2017; 38: E433-42.
51.Aghadavoudi F, Golestanian H, Zarasvand KA. Elastic behaviour of hybrid cross-linked epoxy-based nanocomposite reinforced with GNP and CNT: experimental and multiscale modelling. Polym Bull. 2018: 1-20.
52.Monfared RM, Ayatollahi MR, Isfahani RB. Synergistic effects of hybrid MWCNT/nanosilica on the tensile and tribological properties of woven carbon fabric epoxy composites. Thero Appl Fract Mec. 2018; 96: 272-284.
53.Chan KS, Liang W, Francis WL, Nicolella DP. A multiscale modeling approach to scaffold design and property prediction. J Mech Behav Biomed. 2010; 3(8): 584-593.