The synthesis of silver nanoparticles using the water-in-oil biomicroemulsion method

Document Type: Research Paper


1 Department of Chemical Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran

2 Department of Textile Engineering, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran


Objective(s): A combination of biological and microemulsion methods was used to synthesize silver nanoparticles for the first time. The applied method could be referred to as the biomicroemulsion method, which has the advantages of both biological and the microemulsion methods.
Materials and Methods: In the present study, silver nanoparticles were synthesized in a water-in-oil biomicroemulsion using silver nitrate, which was solubilized in the water core of one microemulsion as the source of silver ions. In addition, a bacterial culture supernatant solubilized in the water core of another microemulsion was employed as the biological reducing agent, dodecane was used as the oil phase, and sodium bis(2-ethylhexyl) sulfosuccinate was applied as the surfactant. Moreover, the antibacterial activity of the nanoparticles was investigated against gram-positive and gram-negative bacteria by disc-diffusion method.
Results: The UV-Vis absorption spectra, dynamic light scattering, and transmission electron microscopy were employed to characterize the presence, size distribution, and morphology of the nanoparticles, respectively. According to the results, the nanoparticles had the optimal conditions in terms of the size and distribution at the silver nitrate concentration of 0.001 M. In addition, the analysis of antibacterial activity indicated that the inhibition zone diameter of Staphylococcus aureus was higher compared to Escherichia coli.
Conclusion: Silver nanoparticles were synthesized successfully using biomicroemulsion method and showed significant anti-bacterial activities against S. aureus and E. coli.


1. Ghorbani HR. Biosynthesis of nanosilver particles using extract of Salmonella typhirium. Arab J Chem. 2017; 10: S1699-S1702.
2. Khodashenas B, Ghorbani HR. Evaluation of the effective factors on size and anti-bacterial properties of biosynthesized silver nanoparticles. Nanomed J. 2015; 6(2): 111-127.
3. Biao L, Tan S, Tan S, Gao J, Liu Z, Fu Y. Synthesis and characterization of proanthocyanins-functionalized Ag nanoparticles. Colloids Surf B. 2018; 169: 438-443.
4. Ghorbani HR, Safekordi AA, Attar H, Sorkhabadi SM. Biological and non-biological methods for silver nanoparticles synthesis. Chem Biochem Eng Q. 2011; 25(3): 317-326.
5. Shaker MA, Shaaban MI. Synthesis of silver nanoparticles with antimicrobial and anti-adherence activities against multidrug-resistant isolates from Acinetobacter baumannii. J Taibah Univ Med Sci. 2017; 12: 291-297.
6. Lopes CRB, Courrol LC. Green synthesis of silver nanoparticles with extract of Mimusops coriacea and light. J Lumin. 2018; 199: 183-187.
7. Ghorbani HR, Molaei M. Antibacterial nanocomposite preparation of polypropylene-Silver using Corona discharge, Prog Org Coat. 2017; 112: 187-190.
8. Behravan MR, Panahi AH, naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial active. Int J Biol Macromol. 2019; 124: 148-154.
9. Khodashenas B, Ghorbani HR. Optimisation of nitrate reductase enzyme activity to synthesise silver nanoparticles, IET nanobiotechnol. 2016; 10(3): 158-161.
10. Das M, Patowary K, Vidya R, Malipeddi H. Microemulsion synthesis of silver nanoparticles using biosurfactant extracted from Pseudomonas aeruginosa MKVIT3 strain and comparison of their antimicrobial and cytotoxic activities. IET Nanobiotechnol. 2016; 10(6): 411-418.
11. Ghorbani HR. Biological coating of paper using silver nanoparticles. IET Nanobiotechnol. 2014; 8(4): 263-266.
12. Zhang W, Qiao X, Chen J, Chen Q. Self-assembly and controlled synthesis of silver nanoparticles in SDS quaternary microemulsion. Mater Lett. 2008; 62: 1689-1692.
13. Niemann B, Rauscher F, Adityawarman D, Voigt A, Sundmacher K. Microemulsion assisted precipitation of particles: experimental and model-based process analysis. Chem Eng Process. 2006; 45(10): 917-935.
14. Kaur G, Dogra V, Kumar R, Kumar S, Singh K. Fabrication of iron oxide nanocolloids using metallosurfactant-based microemulsions: antioxidant activity, cellular, and genotoxicity toward Vitis vinifera. J Biomol Struct Dyn. 2019; 37(4): 892-902.
15. Qing Y, Cheng L, Li R, Liu G, Zhang Y, Tang X, Wang J, Liu H, Qin Y. Potential antibacterial mechanism of silver nanoparticles and the optimization of orthopedic implants by advanced modification technologies. Int J Nanomedicine. 2018; 13: 3311–3327.
16. Ni Z, Wang Z, Sun L, Li B, Zhao Y. Synthesis of poly acrylic acid modified silver nanoparticles and their antimicrobial activities. Mater Sci Eng C Mater Biol Appl. 2014; 41: 249-254.
17. Ghorbani HR, Alizadeh V, Mehr FP, Jafarpourgolroudbary H, Erfan K, Sadeghi Yeganeh S. Preparation of polyurethane/CuO coating film and the study of antifungal activity. Prog Org Coat. 2018; 123: 322-325.
18. Kumar CG, Mamidyala SK, Das B, Sridhar B, Devi GS, Karuna MS. Synthesis of biosurfactant-based silver nanoparticles with purified rhamnolipids isolated from Pseudomonas aeruginosa BS-161R. J Microbiol Biotechnol. 2010; 20(7): 1061-1068.
19. Ghorbani HR, Attar H, Safekordi AA, Sorkhabadi SMR. Design of a bioprocess to produce silver nanoparticles. IET nanobiotechnol. 2012; 6(2): 71-75.
20. Rai M, Ingle AP, Gade A, Duran N. Synthesis of silver nanoparticles by Phoma gardeniae and in vitro evaluation of their efficacy against human disease-causing bacteria and fungi. IET Nanobiotechnol. 2015; 9(2): 71-75.