Recent advances in biological mediated cancer research using silver nanoparticles as a promising strategy for hepatic cancer therapeutics: a systematic review

Document Type: Review Paper


1 Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Student Research Committee, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

3 Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Faculty of Bio & Chemical Engineering, Sathyabama Institute of Science and Technology, Rajiv Gandhi Road, Chennai, Tamilnadu 600119, India

5 Department of Medical Microbiology and Immunology, Division of Biomedical Science, School of Medicine, College of Health Sciences, Mekelle University, 1871 Mekelle, Ethiopia



Nanoparticles are of highlighted interest in scientific research for a wide range of applications as they bridge the gap between atomic structures and bulk materials with unique physicochemical properties. This systematic review was aimed to study the current trends in biological mediated cancer research using biogenic silver nanoparticles (AgNPs) against hepatic cancer cell lines. For this purpose, the electronic databases including Cochrane Library, PubMed, Scopus, Science Direct, ProQuest, Embase, and Web of Science were searched. Forty-six studies passed the eligibility assessments and entered into the current study. All of the studies stated the size distribution of biosynthesized AgNPs below 100 nm with different shapes. Whereas, most studies stated spherical morphology for biogenic AgNPs. Most of the studies (91.30%) represented significant anticancer activity of biogenic AgNPs toward hepatic cancer cell lines. The molecular mechanisms also showed the induction of intracellular Reactive Oxygen Species (ROS) and apoptosis through the biogenic AgNPs-treated hepatic cancer cells. The AgNPs-mediated induction of intracellular ROS overgeneration and ATP synthesis interruption disturb the mitochondria respiratory chain function resulting in the induction of mitochondrial pathway apoptosis. Overall, this systematic review provided strong preliminary evidence representing the efficacy of biogenic AgNPs to combat hepatic cancer cells through in vitro models.


1.Wong CM, Tsang FH, Ng IO. Non-coding RNAs in hepatocellular carcinoma: molecular functions and pathological implications. Nat Rev Gastroenterol Hepatol. 2018; 15(3): 137-151.
2.Llovet JM, Zucman-Rossi J, Pikarsky E, Sangro B, Schwartz M, Sherman M, Gores, G. Hepatocellular carcinoma. Nat Rev Dis Primers. 2016; 2: 16018.
3.Yang JD, Hainaut P, Gores GJ, Amadou A, Plymoth A, Roberts LR. A global view of hepatocellular carcinoma: trends, risk, prevention and management. Nat Rev Gastroenterol Hepatol. 2019; 16(10): 589-604.
4.Llovet JM, Montal R, Sia D, Finn RS. Molecular therapies and precision medicine for hepatocellular carcinoma. Nat Rev Clin Oncol. 2018; 15(10): 599-616.
5.Siegel RL, Miller KD, Jemal A. Cancer statistics, 2019. CA-Cancer J. Clin. 2019; 69(1): 7-34.
6.Gravitz L. Liver cancer. Nature. 2014; 516: S1.
7.Nault JC, Ningarhari M, Rebouissou S, Zucman-Rossi J. The role of telomeres and telomerase in cirrhosis and liver cancer. Nat Rev Gastroenterol Hepatol. 2019; 16(9): 544-558.
8.Bruix J, da Fonseca LG, Reig M. Insights into the success and failure of systemic therapy for hepatocellular carcinoma. Nat. Rev. Gastroenterol. Hepatol. 2019; 16(10): 617-630.
9.Shrivastava S, Dash D. Applying nanotechnology to human health: revolution in biomedical sciences. J Nanotechnol. 2009; 2009: 14.
10.Gnanasekar S, Balakrishnan D, Seetharaman P, Arivalagan P, Chandrasekaran R, Sivaperumal S. Chrysin-anchored silver and gold nanoparticle-reduced graphene oxide composites for breast cancer therapy. ACS Appl Nano Mater. 2020; 3(5): 4574-4585.
11.Singh PK, Jairath G, Ahlawat SS. Nanotechnology: a future tool to improve quality and safety in meat industry. J Food Sci Technol. 2016; 53(4): 1739-1749.
12.Jeevanandam J, Barhoum A, Chan YS, Dufresne A, Danquah MK. Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations. Beilstein J Nanotechnol. 2018; 9: 1050-1074.
13.Richter AP, Brown JS, Bharti B, Wang A, Gangwal S, Houck K, Hubal EAC, Paunov VN, Stoyanov SD, Velev OD. An environmentally benign antimicrobial nanoparticle based on a silver-infused lignin core. Nat Nanotechnol. 2015; 10: 817.
14.Saravanan M, Barik SK, MubarakAli D, Prakash P, Pugazhendhi A. Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria. Microb Pathog. 2018; 116: 221-226.
15.Pugazhendhi A, Prabakar D, Jacob JM, Karuppusamy I, Saratale RG. Synthesis and characterization of silver nanoparticles using Gelidium amansii and its antimicrobial property against various pathogenic bacteria. Microb Pathog. 2018; 114: 41-45.
16.Saravanan M, Arokiyaraj S, Lakshmi T, Pugazhendhi A. Synthesis of silver nanoparticles from Phenerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria. Microb Pathog. 2018; 117: 68-72.
17.Suwan T, Khongkhunthian S, Okonogi S. Antifungal activity of polymeric micelles of silver nanoparticles prepared from Psidium guajava aqueous extract. Drug Discoveries Ther. 2019; 13(2): 62-69.
18.Hu X, Saravanakumar K, Jin T, Wang M-H. Mycosynthesis, characterization, anticancer and antibacterial activity of silver nanoparticles from endophytic fungus Talaromyces purpureogenus. Int J Nanomed. 2019; 14: 3427-3438.
19.Pugazhendhi A, Edison TNJI, Karuppusamy I, Kathirvel B. Inorganic nanoparticles: A potential cancer therapy for human welfare. Int J Pharm. 2018; 539(1): 104-111.
20.Samuel MS, Jose S, Selvarajan E, Mathimani T, Pugazhendhi A. Biosynthesized silver nanoparticles using Bacillus amyloliquefaciens; Application for cytotoxicity effect on A549 cell line and photocatalytic degradation of p-nitrophenol. J Photochem Photobiol., B. 2020; 202: 111642.
21.Marimuthu S, Antonisamy AJ, Malayandi S, Rajendran K, Tsai P-C, Pugazhendhi A, Ponnusamy VK. Silver nanoparticles in dye effluent treatment: A review on synthesis, treatment methods, mechanisms, photocatalytic degradation, toxic effects and mitigation of toxicity. J Photochem Photobiol B. 2020; 205: 111823.
22.Shanmuganathan R, Mubarak Ali D, Prabakar D, Muthukumar H, Thajuddin N, Kumar SS, Pugazhendhi A. An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach. Environ Sci Pollut Res. 2018; 25(11): 10362-10370.
23.Shanmuganathan R, Karuppusamy I, Saravanan M, Muthukumar H, Ponnuchamy K, Ramkumar V, Pugazhendhi A. Synthesis of silver nanoparticles and their biomedical applications-a comprehensive review. Curr Pharm Des. 2019; 25(24): 2650.
24.Khalid S, Hanif R. Green biosynthesis of silver nanoparticles conjugated to gefitinib as delivery vehicle. Int J Adv Sci Eng Technol. 2017; 5(2): 59-63.
25.Naz M, Nasiri N, Ikram M, Nafees M, Qureshi MZ, Ali S, Tricoli A. Eco-friendly biosynthesis, anticancer drug loading and cytotoxic effect of capped Ag-nanoparticles against breast cancer. Appl Nanosci. 2017; 7(8): 793-802.
26.Patrizia Di P, Gaetano S, Lidia Z, Cristina S. Gold and silver nanoparticles for applications in theranostics. Curr Top Med Chem. 2016; 16(27): 3069-3102.
27.Gudikandula K, Charya Maringanti S. Synthesis of silver nanoparticles by chemical and biological methods and their antimicrobial properties. J Exp Nanosci. 2016; 11(9): 714-721.
28.Zhang XF, Liu ZG, Shen W, Gurunathan S. Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. Int J Mol Sci. 2016; 17(9): 1534.
29.Jacob JM, John MS, Jacob A, Abitha P, Kumar SS, Rajan R, Natarajan S, Pugazhendhi A. Bactericidal coating of paper towels via sustainable biosynthesis of silver nanoparticles using Ocimum sanctum leaf extract. Mater Res Express. 2019; 6(4): 045401.
30.Siddiqi KS, Husen A, Rao RAK. A review on biosynthesis of silver nanoparticles and their biocidal properties. J Nanobiotechnology. 2018; 16(1): 14.
31.Barabadi H, Kobarfard F, Vahidi H. Biosynthesis and characterization of biogenic tellurium nanoparticles by using Penicillium chrysogenum PTCC 5031: A novel approach in gold biotechnology. Iran J Pharm Res. 2018; 17(Special Issue 2): 87-97.
32.Rezvani Amin Z, Khashyarmanesh Z, Fazly Bazzaz BS, Sabeti Noghabi Z. Does biosynthetic silver nanoparticles are more stable with lower toxicity than their synthetic counterparts? Iran J Pharm Res. 2019; 18(1): 210-221.
33.Abbas Q, Saleem M, Phull AR, Rafiq M, Hassan M, Lee KH, Seo SY. Green synthesis of silver nanoparticles using Bidens frondosa extract and their tyrosinase activity. Iran J Pharm Res. 2017; 16(2): 760-767.
34.Dobrucka R. Synthesis of titanium dioxide nanoparticles using Echinacea purpurea herba. Iran J Pharm Res. 2017; 16(2): 753-759.
35.Karimi N, Chardoli A, Fattahi A. Biosynthesis, characterization, antimicrobial and cytotoxic effects of silver nanoparticles using Nigella arvensis seed extract. Iran J Pharm Res. 2017; 16(3): 1167-1175.
36.Or Rashid MM, Islam MS, Haque MA, Rahman MA, Hossain MT, Hamid MA. Antibacterial activity of polyaniline coated silver nanoparticles synthesized from Piper betle leaves extract. Iran J Pharm Res. 2016; 15(2): 591-597.
37.Salari S, Esmaeilzadeh Bahabadi S, Samzadeh-Kermani A, Yousefzaei F. In vitro evaluation of antioxidant and antibacterial potential of green synthesized silver nanoparticles using Prosopis farcta fruit extract. Iran J Pharm Res. 2019; 18(1): 430-445.
38.Ahn EY, Hwang SJ, Choi MJ, Cho S, Lee HJ, Park Y. Upcycling of jellyfish (Nemopilema nomurai) sea wastes as highly valuable reducing agents for green synthesis of gold nanoparticles and their antitumor and anti-inflammatory activity. Artif Cells Nanomed. Biotechnol. 2018; 46(sup2): 1127-1136.
39.Lee SH, Jun B-H. Silver nanoparticles: synthesis and application for nanomedicine. Int J Mol Sci. 2019; 20(4): 865.
40.Rajkumar T, Sapi A, Das G, Debnath T, Ansari A, Patra JK. Biosynthesis of silver nanoparticle using extract of Zea mays (corn flour) and investigation of its cytotoxicity effect and radical scavenging potential. J Photochem Photobiol B. 2019; 193: 1-7.
41.Qasim Nasar M, Zohra T, Khalil AT, Saqib S, Ayaz M, Ahmad A, Shinwari ZK. Seripheidium quettense mediated green synthesis of biogenic silver nanoparticles and their theranostic applications. Green Chem Lett Rev. 2019; 12(3): 310-322.
42.Patra JK, Das G, Shin HS. Facile green biosynthesis of silver nanoparticles using Pisum sativum L. outer peel aqueous extract and its antidiabetic, cytotoxicity, antioxidant, and antibacterial activity. Int J Nanomed. 2019; 14: 6679-6690.
43.Pathak M, Tyagi P, Punia A, Singh L. Cytotoxic action of silver nanoparticles synthesized from Phyllanthus fraternus on hepatic and breast cancer cell lines: A green approach. Int J Green Pharm. 2019; 13(3): 229-235.
44.Nasar MQ, Khalil AT, Ali M, Shah M, Ayaz M, Shinwari ZK. Phytochemical analysis, ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles, their cytotoxic and antimicrobial potentials. Medicina. 2019; 55(7): 369.
45.Ibrahim FY, El-Khateeb AY, Mohamed AH. Rhus and safflower extracts as potential novel food antioxidant, anticancer, and antimicrobial agents using nanotechnology. Foods. 2019; 8(4): 139.
46.Botha TL, Elemike EE, Horn S, Onwudiwe DC, Giesy JP, Wepener V. Cytotoxicity of Ag, Au and Ag-Au bimetallic nanoparticles prepared using golden rod (Solidago canadensis) plant extract. Sci Rep. 2019; 9(1): 1-8.
47.Bhatnagar S, Kobori T, Ganesh D, Ogawa K, Aoyagi H. Biosynthesis of silver nanoparticles mediated by extracellular pigment from Talaromyces purpurogenus and their biomedical applications. Nanomaterials. 2019; 9(7): 1042.
48.Aziz N, Faraz M, Sherwani MA, Fatma T, Prasad R. Illuminating the anticancerous efficacy of a new fungal chassis for silver nanoparticle synthesis. Front Chem. 2019; 7: 65.
49.Abbasi BH, Nazir M, Muhammad W, Hashmi SS, Abbasi R, Rahman L, Hano C. A comparative evaluation of the antiproliferative activity against HepG2 liver carcinoma cells of plant-derived silver nanoparticles from basil extracts with contrasting anthocyanin contents. Biomolecules. 2019; 9(8): 320.
50.Moher D, Liberati A, Tetzlaff J, Altman DG. Preferred reporting items for systematic reviews and meta-analyses: the PRISMA statement. PLoS Med. 2009; 6(7): e1000097.
51.Singh D, Kumar V, Yadav E, Falls N, Singh M, Komal U, Verma A. One-pot green synthesis and structural characterisation of silver nanoparticles using aqueous leaves extract of Carissa carandas: antioxidant, anticancer and antibacterial activities. IET Nanobiotechnol. 2018; 12(6): 748-756.
52.Singh A, Dar MY, Joshi B, Sharma B, Shrivastava S, Shukla S. Phytofabrication of silver nanoparticles: novel drug to overcome hepatocellular ailments. Toxicol Rep. 2018; 5: 333-342.
53.Saratale RG, Shin HS, Kumar G, Benelli G, Kim DS, Saratale GD. Exploiting antidiabetic activity of silver nanoparticles synthesized using Punica granatum leaves and anticancer potential against human liver cancer cells (HepG2). Artif Cells Nanomed Biotechnol. 2018; 46(1): 211-222.
54.Saratale RG, Benelli G, Kumar G, Kim DS, Saratale GD. Bio-fabrication of silver nanoparticles using the leaf extract of an ancient herbal medicine, dandelion (Taraxacum officinale), evaluation of their antioxidant, anticancer potential, and antimicrobial activity against phytopathogens. Environ. Sci Pollut Res. 2018; 25(11): 10392-10406.
55.Raghuwanshi N, Patel A, Arora N, Varshney R, Srivastava AK, Pruthi V. Antineoplastic and antimicrobial potential of novel phytofabricated silver nanoparticles from Pterospermum acerifolium leaf extract. Nanosci Nanotechnol-Asia. 2018; 8(2): 297-308.
56.Paul Das M, Rebecca Livingstone J, Veluswamy P, Das J. Exploration of Wedelia chinensis leaf-assisted silver nanoparticles for antioxidant, antibacterial and in vitro cytotoxic applications. J Food Drug Anal. 2018; 26(2): 917-925.
57.Patra JK, Das G, Kumar A, Ansari A, Kim H, Shin H-S. Photo-mediated biosynthesis of silver nanoparticles using the non-edible accrescent fruiting calyx of Physalis peruviana L. fruits and investigation of its radical scavenging potential and cytotoxicity activities. J Photochem Photobiol., B. 2018; 188: 116-125.
58.Padinjarathil H, Joseph MM, Unnikrishnan BS, Preethi GU, Shiji R, Archana MG, Maya S, Syama HP, Sreelekha TT. Galactomannan endowed biogenic silver nanoparticles exposed enhanced cancer cytotoxicity with excellent biocompatibility. Int J Biol Macromol. 2018; 118: 1174-1182.
59.Ovais M, Khalil AT, Raza A, Islam NU, Ayaz M, Saravanan M, Ali M, Ahmad I, Shahid M, Shinwari ZK. Multifunctional theranostic applications of biocompatible green-synthesized colloidal nanoparticles. Appl Microbiol Biotechnol. 2018; 102(10): 4393-4408.
60.Khan SA, Bello BA, Khan JA, Anwar Y, Mirza MB, Qadri F, Farooq A, Adam IK, Asiri AM, Khan SB. Albizia chevalier based Ag nanoparticles: anti-proliferation, bactericidal and pollutants degradation performance. J Photochem Photobiol B. 2018; 182: 62-70.
61.Kahsay MH, RamaDevi D, Kumar YP, Mohan BS, Tadesse A, Battu G, Basavaiah K. Synthesis of silver nanoparticles using aqueous extract of Dolichos lablab for reduction of 4-Nitrophenol, antimicrobial and anticancer activities. OpenNano. 2018; 3: 28-37.
62.Jadhav K, Deore S, Dhamecha D, Hr R, Jagwani S, Jalalpure S, Bohara R. Phytosynthesis of silver nanoparticles: characterization, biocompatibility studies, and anticancer activity. ACS Biomater Sci Eng. 2018; 4(3): 892-899.
63.Dhayalan M, Denison MIJ, Ayyar M, Gandhi NN, Krishnan K, Abdulhadi B. Biogenic synthesis, characterization of gold and silver nanoparticles from Coleus forskohlii and their clinical importance. J Photochem Photobiol B. 2018; 183: 251-257.
64.Yassin AM, El-Deeb NM, Metwaly AM, El Fawal GF, Radwan MM, Hafez EE. Induction of apoptosis in human cancer cells through extrinsic and intrinsic pathways by Balanites aegyptiaca furostanol saponins and saponin-coated silver nanoparticles. Appl Biochem Biotechnol. 2017; 182(4): 1675-1693.
65.Ramkumar SS, Sivakumar N, Selvakumar G, Selvankumar T, Sudhakar C, Ashokkumar B, Karthi S. Green synthesized silver nanoparticles from: Garcinia imberti bourd and their impact on root canal pathogens and HepG2 cell lines. RSC Adv. 2017; 7(55): 34548-34555.
66.Sheet S, Sathishkumar Y, Sivakumar AS, Shim KS, Lee YS. Low-shear-modeled microgravity-grown Penicillium chrysogenum-mediated biosynthesis of silver nanoparticles with enhanced antimicrobial activity and its anticancer effect in human liver cancer and fibroblast cells. Bioprocess Biosyst Eng. 2017; 40(10): 1529-1542.
67.Shanmugasundaram T, Radhakrishnan M, Gopikrishnan V, Kadirvelu K, Balagurunathan R. Biocompatible silver, gold and silver/gold alloy nanoparticles for enhanced cancer therapy: in vitro and in vivo perspectives. Nanoscale. 2017; 9(43): 16773-16790.
68.Prasannaraj G, Venkatachalam P. Green engineering of biomolecule-coated metallic silver nanoparticles and their potential cytotoxic activity against cancer cell lines. Adv Nat Sci Nanosci Nanotechnol. 2017; 8(2): 025001.
69.Prasannaraj G, Sahi SV, Benelli G, Venkatachalam P. Coating with active phytomolecules enhances anticancer activity of bio-engineered Ag nanocomplex. J Clust Sci. 2017; 28(4): 2349-2367.
70.Khalid M, Khalid N, Ahmed I, Hanif R, Ismail M, Janjua HA. Comparative studies of three novel freshwater microalgae strains for synthesis of silver nanoparticles: insights of characterization, antibacterial, cytotoxicity and antiviral activities. J Appl Phycol. 2017; 29(4): 1851-1863.
71.Karunagaran V, Rajendran K, Sen S. Optimization of biosynthesis of silver oxide nanoparticles and its anticancer activity. Int. J. Nanosci. 2017; 16(5-6): 1750018.
72.He Y, Li X, Wang J, Yang Q, Yao B, Zhao Y, Zhao A, Sun W, Zhang Q. Synthesis, characterization and evaluation cytotoxic activity of silver nanoparticles synthesized by Chinese herbal Cornus officinalis via environment friendly approach. Environ. Toxicol. Pharmacol. 2017; 56: 56-60.
73.Gowri Shankar K, Pradhan N, Masilamani K, Fleming AT. Silver nanoparticles from Trianthema portulacastrum: Green synthesis, characterization, antibacterial and anticancer properties. Asian J Pharm Clin Res. 2017; 10(3): 306-313.
74.Gomaa EZ. Antimicrobial, antioxidant and antitumor activities of silver nanoparticles synthesized by Allium cepa extract: A green approach. J Genet Eng Biotechnol. 2017; 15(1): 49-57.
75.El-Hela AA, Abdelhady NM, Gonaid MH, Badr KA. Antioxidant, cytotoxic and antimicrobial activities of crude and green synthesized silver nanoparticles′ extracts of Crataegus sinaica bioss. Leaves. Int J Pharm Sci Rev Res. 2017; 45(1): 223-232.
76.Bello BA, Khan SA, Khan JA, Syed FQ, Anwar Y, Khan SB. Antiproliferation and antibacterial effect of biosynthesized AgNps from leaves extract of Guiera senegalensis and its catalytic reduction on some persistent organic pollutants. J. Photochem. Photobiol., B. 2017; 175: 99-108.
77.Xia QH, Ma YJ, Wang JW. Biosynthesis of silver nanoparticles using Taxus yunnanensis callus and their antibacterial activity and cytotoxicity in human cancer cells. Nanomaterials. 2016; 6(9): 160.
78.Supraja N, Prasad T, Soundariya M, Babujanarthanam R. Synthesis, characterization and dose dependent antimicrobial and anticancerous activity of phycogenic silver nanoparticles against human hepatic carcinoma (HepG2) cell line. AIMS Bioeng. 2016; 3(4): 425-440.
79.Rajeshkumar S, Malarkodi C, Vanaja M, Annadurai G. Anticancer and enhanced antimicrobial activity of biosynthesizd silver nanoparticles against clinical pathogens. J Mol Struct. 2016; 1116: 165-173.
80.Kumar B, Smita K, Seqqat R, Benalcazar K, Grijalva M, Cumbal L. In vitro evaluation of silver nanoparticles cytotoxicity on Hepatic cancer (Hep-G2) cell line and their antioxidant activity: Green approach for fabrication and application. J Photochem Photobiol B. 2016; 159: 8-13.
81.Jaganathan A, Murugan K, Panneerselvam C, Madhiyazhagan P, Dinesh D, Vadivalagan C, Chandramohan B, Suresh U, Rajaganesh R, Subramaniam J, Nicoletti M. Earthworm-mediated synthesis of silver nanoparticles: A potent tool against hepatocellular carcinoma, Plasmodium falciparum parasites and malaria mosquitoes. Parasitol Int. 2016; 65(3): 276-284.
82.Ebrahiminezhad A, Bagheri M, Taghizadeh S-M, Berenjian A, Ghasemi Y. Biomimetic synthesis of silver nanoparticles using microalgal secretory carbohydrates as a novel anticancer and antimicrobial. Adv Nat Sci Nanosci Nanotechnol. 2016; 7(1): 015018.
83.Castro-Aceituno V, Ahn S, Simu SY, Singh P, Mathiyalagan R, Lee HA, Yang DC. Anticancer activity of silver nanoparticles from Panax ginseng fresh leaves in human cancer cells. Biomed. Pharmacother. 2016; 84: 158-165.
84.Abd-Elnaby HM, Abo-Elala GM, Abdel-Raouf UM, Hamed MM. Antibacterial and anticancer activity of extracellular synthesized silver nanoparticles from marine Streptomyces rochei MHM13. Egypt. J. Aquat. Res. 2016; 42(3): 301-312.
85.Rathi Sre PR, Reka M, Poovazhagi R, Arul Kumar M, Murugesan K. Antibacterial and cytotoxic effect of biologically synthesized silver nanoparticles using aqueous root extract of Erythrina indica lam. Spectrochimica acta Part A, Molecular and biomolecular spectroscopy. 2015; 135: 1137-1144.
86.Abdel-Fattah WI, Eid MM, Hanafy MF, Hussein M, Abd El-Moez SI, El-Hallouty SM, Mohamed E. Verification of resistance to three mediated microbial strains and cancerous defense against MCF7 compared to HepG2 through microwave synthesized plant-mediated silver nanoparticle. Adv. Nat. Sci.: Nanosci. Nanotechnol. 2015; 6(3): 035002.
87.Inbathamizh L, Ponnu TM, Mary EJ. In vitro evaluation of antioxidant and anticancer potential of Morinda pubescens synthesized silver nanoparticles. J. Pharm. Res. 2013; 6(1): 32-38.
88.Gahlawat G, Choudhury AR. A review on the biosynthesis of metal and metal salt nanoparticles by microbes. RSC Adv. 2019; 9(23): 12944-12967.
89.Hamouda RA, Hussein MH, Abo-elmagd RA, Bawazir SS. Synthesis and biological characterization of silver nanoparticles derived from the cyanobacterium Oscillatoria limnetica. Sci Rep. 2019; 9(1): 13071.
90.Barabadi H, Honary S. Biofabrication of gold and silver nanoparticles for pharmaceutical applications. Pharm. Biomed. Res. 2016; 2(1): 1-7.
91.Roy A, Bulut O, Some S, Mandal AK, Yilmaz MD. Green synthesis of silver nanoparticles: biomolecule-nanoparticle organizations targeting antimicrobial activity. RSC Adv. 2019; 9(5): 2673-2702.
92.Tekin V, Kozgus Guldu O, Dervis E, Yurt Kilcar A, Uygur E, Biber Muftuler FZ. Green synthesis of silver nanoparticles by using eugenol and evaluation of antimicrobial potential. Appl. Organomet. Chem. 2019; 33(7): e4969.
93.Yesilot S, Aydin C. Silver nanoparticles; a new hope in cancer therapy? East. J. Med. 2019; 24(1): 111-116.
94.Ding J, Chen G, Chen G, Guo M. One-pot synthesis of epirubicin-capped silver nanoparticles and their anticancer activity against hepG2 cells. Pharmaceutics. 2019; 11(3): 123.
95.Zhang X-F, Shen W, Gurunathan S. Silver nanoparticle-mediated cellular responses in various cell lines: an in vitro model. Int. J. Mol. Sci. 2016; 17(10): 1603.
96.Barabadi H, Vahidi H, Damavandi Kamali K, Rashedi M, Saravanan M. Antineoplastic biogenic silver nanomaterials to combat cervical cancer: a novel approach in cancer therapeutics. J Clust Sci. 2020; 31: 659–672.
97.Murphy A, Casey A, Byrne G, Chambers G, Howe O. Silver nanoparticles induce pro‐inflammatory gene expression and inflammasome activation in human monocytes. J. Appl. Toxicol. 2016; 36(10): 1311-1320.
98.Nishanth RP, Jyotsna RG, Schlager JJ, Hussain SM, Reddanna P. Inflammatory responses of RAW 264.7 macrophages upon exposure to nanoparticles: role of ROS-NFκB signaling pathway. Nanotoxicology. 2011; 5(4): 502-516.
99.Cheng X, Zhang W, Ji Y, Meng J, Guo H, Liu J, Wu X, Xu H. Revealing silver cytotoxicity using Au nanorods/Ag shell nanostructures: disrupting cell membrane and causing apoptosis through oxidative damage. RSC Adv. 2013; 3(7): 2296-2305.
100.Ravindran A, Chandrasekaran N, Mukherjee A. Studies on differential behavior of silver nanoparticles towards thiol containing amino acids. Curr. Nanosci. 2012; 8(1): 141-149.
101.Jadhav K, Deore S, Dhamecha D, Jagwani S, Jalalpure S, Bohara R. Phytosynthesis of silver nanoparticles: characterization, biocompatibility studies, and anticancer activity. ACS Biomater. Sci. Eng. 2018; 4(3): 892-899.
102.Gorshkov V, Bubis JA, Solovyeva EM, Gorshkov MV, Kjeldsen F. Protein corona formed on silver nanoparticles in blood plasma is highly selective and resistant to physicochemical changes of the solution. Environ. Sci.: Nano. 2019; 6(4): 1089-1098.
103.Durán N, Silveira CP, Durán M, Martinez DST. Silver nanoparticle protein corona and toxicity: a mini-review. J Nanobiotechnology. 2015; 13(1): 55.
104.Li L, Zhang W, Desikan Seshadri VD, Cao G. Synthesis and characterization of gold nanoparticles from Marsdenia tenacissima and its anticancer activity of liver cancer HepG2 cells. Artif. Cells Nanomed Biotechnol. 2019; 47(1): 3029-3036.
105.Rajeshkumar S. Anticancer activity of eco-friendly gold nanoparticles against lung and liver cancer cells. J Genet Eng Biotechnol. 2016; 14(1): 195-202.
106.Bisht G, Rayamajhi S. ZnO nanoparticles: a promising anticancer agent. Nanobiomedicine. 2016; 3(Godište 2016): 3-9.
107.Xia Y, Zhong J, Zhao M, Tang Y, Han N, Hua L, Xu T, Wang C, Zhu B. Galactose-modified selenium nanoparticles for targeted delivery of doxorubicin to hepatocellular carcinoma. Drug Deliv. 2019; 26(1): 1-11.