The evaluation of the cortex neurons viability in CdS nanoparticles induced toxicity

Document Type : Research Paper

Authors

1 Department of Molecular Biology and Genetics, Biotechnology, Gaziosmanpasa University, Postal Code 60250, Tokat, Turkey

2 Department of Nanoscience and Nanoengineering, Institute of Naturel and Applied Sciences, Ataturk University, Postal Code 25240, Erzurum, Turkey

3 Department of Pharmacology and Toxicology, Faculty of Veterinary Science, Atatürk University, Postal Code 25240, Erzurum, Turkey

4 Department of Biology, Faculty of Science, Atatürk University, Postal Code 25240, Erzurum, Turkey

5 Department of Medical Pharmacology, Faculty of Medicine, Ataturk University, Postal Code 25240, Erzurum, Turkey

Abstract

Objective(s): Cadmium sulfur (CdS) is a type of quantum dot which is a unique light-emitting semiconductor nanocrystal. Quantum dots have wide applications in optoelectronics, solar cells, biology, and medicine fields.
Materials and Methods: Morphological properties and structural analysis for CdS were tested by using different methods (TEM, XPS and XRD).  Cortical neuron cells were used for toxicity investigations. The cells were treated with different concentrations of CdS (100, 10, 1, 0.1, 0.01 µg/mL) and incubated for 24 h (5% CO2; 37°C). In vitro studies were done by examining cellular viability (MTT assay) and oxidative stress/status (TAC/TOS).
Results: According to our results, the increasing concentration of CdS resulted in decreased cell viability. Total antioxidant capacity (TAC) of neurons increased following exposure to the lowest concentrations of CdS. In addition, inverse to our TAC findings, total oxidant status (TOS) was decreased following exposure to lower concentrations of CdS.
Conclusion: Recently, because of advances in diagnostic and drug delivery systems ingestion rate of CdS by humans were increased. Hence, this study aimed to investigate the toxic effects of CdS on Cortex Neurons cell cultures. The production of CdS quantum dot particles was done by using the Viridibacillus arenosi K64 (biosynthesis method) which provides environmentally friendly, economical, reliable, and controlled production.

Keywords

References

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Nanomedicine Journal
Volume 8, Issue 3
July 2021
Pages 211-219

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