Synthesis and evaluation of bactericidal properties of CuO nanoparticles against Aeromonas hydrophila


1 Department of Natural resources, Savadkooh Branch, Islamic Azad University, Savadkooh, Iran

2 Department of Fishery, Qaemshahr, Branch, Islamic Azad University, Qaemshahr, Iran



CuO is one of the most important transition metal oxides due to its captivating properties. It is used in various technological applications such as high critical temperature superconductors, gas sensors, in photoconductive applications, and so on. Recently, it has been used as an antimicrobial agent against various bacterial species.
Materials and Methods:
Here, we synthesized CuO nanoparticles (NPs) and explored the antibacterial activity of CuO NPs preparation.
Single crystalline nanoparticles of copper oxide having almost uniform particle size of 5-6 nm has been synthesized by a facile and versatile route. XRD spectra confirmed the formation of single phase CuO NPs. Transmission electron microscopy results corroborate well with XRD results. The technique employed is free from toxic solvents, organics and amines, is based on a simple reaction of copper sulfate and de-ionized water (DI), and their bactericidal effects against of Aeromonas hydrophila ATCC 7966T bacteria were investigated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) with liquid culture for all of the Aeromonas hydrophila culture Medias was done.  
Present study confirms that Copper oxide nanoparticles have great promise as antimicrobial agent against Aeromonas hydrophila.


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