Production of gold nanoparticles by Streptomyces djakartensis isolate B-5

Document Type: Research Paper

Authors

1 Department of Agricultural Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Iran

2 Department of Plant pathology, College of Agriculture, Shahid Bahonar University of Kerman, Iran

3 Department of Agricultural Biotechnology, College of Agriculture, Hormozgan University, Iran

10.7508/nmj.2015.04.003

Abstract

Objective(s): 
Biosynthesis of gold nanoparticles (NGPs) is environmentally safer than chemical and physical procedures. This method requires no use of toxic solvents and synthesis of dangerous products and is environmentally safe. In this study, we report the biosynthesis of NGPs using Streptomyces djakartensis
isolate B-5.  
Materials and Methods:
NGPs were biosynthesized by reducing aqueous gold chloride solution via a Streptomyces isolate without the need for any additive for protecting nanoparticles from aggregation. We characterized the responsible Streptomycete; its genome DNA was isolated, purified and 16S rRNA was amplified by PCR. The amplified isolate was sequenced; using the BLAST search tool from NCBI, the microorganism was identified to species level.  

Results:
Treating chloroauric acid solutions with this bacterium resulted in reduction of gold ions and formation of stable NGPs. TEM and SEM electro micrographs of NGPs indicated size range from 2- 25 nm with average of 9.09 nm produced intracellular by the bacterium. SEM electro micrographs revealed morphology of spores and mycelia. The amplified PCR fragment of 16S rRNA gene was cloned and sequenced from both sides; it consisted of 741 nucleotides. According to NCBI GenBank, the bacterium had 97.1% homology with Streptomyces djakartensis strain RT-49. The GenBank accession number for partial 16S rRNA gene was recorded as JX162550.  

Conclusion:
Optimized application of such findings may create applications of Streptomycetes for use as bio-factories in eco-friendly production of NGPs to serve in demanding industries and related biomedical areas. Research in this area should also focus on the unlocking the full mechanism of NGPs biosynthesis by Streptomycetes.

Keywords


1. Kohler JM, Csaki A, Reichert R, Straube W, Fritzche W. Selective labeling of oligonucleotide monolayers by metallic nanobeads for fast optical readout of DNA-chips. Sens Actuators B Chem. 2001; 76(1-3): 166-172.

2. Schatz GC, Lazarides AA, Kelly KL, Jensen TR. Optical properties of metal nanoparticles aggregates important in biosensors. J Mol Struct (Theochem). 2000; 529(1-3): 59-63.

3. Rosi NL, Mirkin CA. Nanostructures in biodiagnostics. Chem Rev. 2005; 105: 1547-1562.

4. Ferrari M. Cancer nanotechnology: opportunities and challenges. Nat Rev Cancer. 2005; 5(3): 161-171.

5. Murphy CJ, Gole AM, Stone JW, Sisco PN, Alkilany AM, Goldsmith EC, et al. Gold nanoparticles in biology: beyond toxicity to cellular imaging. Acc Chem Res. 2008; 41(12): 1721-1730.

6. Liu YL, Shipton MK, Ryan J, Kaufman ED, Franzen S, Feldheim DL. Synthesis, stability, and cellular internalization of gold nanoparticles containing mixed peptide-poly (ethylene glycol) monolayers. Anal Chem. 2007; 79(6): 2221–2229.

7. Khan JA, Pillai B, Das TK, Singh Y, Maiti S. Molecular effects of uptake of gold nanoparticles in HeLa cells. ChemBioChem. 2007; 8(11): 1237-1240.

8. Han G, Ghosh P, Rotello VM. Functionalized gold nanoparticles for drug delivery. Nanomedicine (Lond). 2007; 2(1): 113-123.

9. Sarin H, Kanevsky AS, Wu H, Brimacombe KR, Fung SH, Sousa AA, et al. Effective transvascular delivery of nanoparticles across the blood-brain tumor barrier into malignant glioma cells. J Transl Med. 2008; 6: 1-15.

10. Raoof M, Corr SJ, Kaluarachchi WD, Massey KL, Briggs K, Zhu C, et al. Stability of antibody-conjugated gold nanoparticles in the endolysosomal nanoenvironment: implications for noninvasive radiofrequency-based cancer therapy. Nanomedicine. 2012; 8(7): 1096-1105.

11. Eghtedari M, Liopo AV, Copland JA, Oraevslty AA, Motamedi M. Engineering of hetero-functional gold nanorods for the in vivo molecular targeting of breast cancer cells. Nano Lett. 2009; 9(1): 287-291.

12. Bonjar LS. "Nanogold detoxifying machine" to remove idle nanogold particles from blood stream of cancer patients treated with antibody-nanogold therapeutics. Med Hypotheses. 2013; 80(5): 601-605.

13. Sadowski Z. Biosynthesis and Application of Silver and Gold Nanoparticles In: Perez DP, editor. Silver Nanoparticles. China: InTech; 2010.

14. Newman D K, Kolter R. A role for excreted quinones in extracellular electron transfer. Nature. 2000; 405(6782): 94-97.

15. Sapkal MR, Deshmukh AM. Biosynthesis of Gold Nanoparticles by Streptomyces species. Res J Biotech. 2008; 3(2): 36-39.

16. Mann S. Biomimetic Materials Chemistry. New York: Weinheim, Cambridge, VCH Publishers; 1996.

17. Kemp MM, Kumar A, Mousa S, Park TJ, Ajayan P, Kubotera N, et al. Synthesis of gold and silver nanoparticles stabilized with glycosaminoglycans having distinctive biological activities. Biomacromolecules. 2009; 10(3): 589-595.

18. Lee JY, Hwang BK. Diversity of antifungal Actinomycetes in various vegetative soils of Korea. Can J Microbiol. 2002; 48(5): 407-417.

19. Dhingra OD, Sinclair JB. Basic plant pathology methods. USA: CRC Press; 1995.

20. Mandal, D, Bolander ME, Mukhopadhyay D, Sarkar G, Mukherjee P. The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbiol Biotechnol. 2006; 69(5): 485-492.

21. 21.Gehring AM, Wang ST, Kearns DB, Storer NY, Losicki R. Novel Genes That Influence Development in Streptomyces coelicolor. J Bacteriol. 2004; 186(11): 3570- 3577.

22. Baharlouei A, Sharifi-Sirchi GR, Shahidi Bonjar GH. Identification of an Antifungal Chitinase from a Potential Biocontrol Agent, Streptomyces plicatus strain 101, and it’s New Antagonistic Spectrum of Activity. Philipp Agric Sci. 2010; 93(4): 439-445.

23. Sambrook J, Russel DW. Molecular cloning: a laboratory manual (3rd). New York: Cold Spring Harbor Laboratory Press; 2001.

24. Kitching M. Biosynthesis of Nano-gold Particles in Fungi. Dublin City University, 2011. Retrieved April 12, 2013 from: http://www.scribd.com/doc/104508376/Biosynthesus-of-Nano-gold-Particles-by-Fungi

25. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA. Practical Streptomyces Genetics (2nd ed).