The effects of zinc oxide nanoparticles on differentiation of human mesenchymal stem cells to osteoblast

Authors

1 Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

2 payam e nour university, biology branch

10.7508/nmj.2015.05.004

Abstract

Objective(s):
The mesenchymal stem cells (MSCs) have been introduced as appropriate cells for tissue engineering and medical applications. Some studies have shown that topography of materials especially physical surface characteristics and particles size could enhance adhesion and proliferation of osteoblasts. In the present research, we studied the distinction effect of 30 and 60 μg/ml of zinc oxide (ZnO) on differentiation of human mesenchymal stem cells to osteoblast.
Materials and Methods:
After the third passage, human bone marrow mesenchymal stem cells were exposed to 30 and 60 μg/ml of ZnO nanoparticles having a size of 30 nm. The control group has received no ZnO nanoparticles. On day 15 of incubation for monitoring the cellular differentiation, alizarin red staining and RT-PCR assays were performed to evaluate the level of osteopontin, osteocalsin and alkaline phosphatase genes expression.
Results:
In the group receiving 30 μg/ml of ZnO nanoparticles, the expression of osteogenic markers such as alkaline phosphatase, osteocalcin and osteopontin genes were significantly higher than both control and the group receiving 60 μg/ml ZnO nanoparticle. These data also confirmed by alizarin red staining.
Conclusion:
It seems the process of differentiation of MSCs affected by ZnO nanoparticles is dependent on dose as well as on the size of ZnO.

Keywords


  1. Kaur S, Singhal B. When nano meets stem: the impact of nanotechnology in stem cell biology. J Biosci Bioeng. 2012; 113(1): 1-4.
  2. Arora P, Sindhu A, Dilbaghi N, Chaudhury A, Rajakumar G, Rahuman AA. Nano-regenerative medicine towards clinical outcome of stem cell and tissue engineering in humans. J Cell Mol Med. 2012; 16(9): 1991-2000.
  3. Wang ZL. Splendid one-dimensional nanostructures of zinc oxide: a new nanomaterial family for nanotechnology. ACS Nano. 2008; 2(10): 1987-1992.
  4. Noori-Daloii MR, Ghofrani M. Nanotechnology in laboratory diagnosis and molecular medicine: The importance and outlook, a review article. J Nanotech. 2008; 6(123): 596-608.
  5. Eslaminejad MB, Salami F, Mehranjani MS, Abnoosi MH. Study of BIO (6-Bromoindirubin-3᾽-Oxim) effect on growth and bone differentiation of rat marrow-derived mesenchymal stem cells. J Hamedan Uni Med Sci . 2009; 4: 5-13.
  1. Deng X, Luan Q, Chen W, Wang Y, Wu M, Zhang H, et al. Nanosized zinc oxide particles induce neural stem cell apoptosis. Nanotechnology. 2009; 20(11): 115101. z
  2. Dulgar-Tulloch AJ, Bizios R, Siegel RW. Differentiation of human mesenchymal stem cells on nano- and micro- grain size titania. Mater Sci Eng C Mater Biol Appl. 2011; 31(2): 357-362.
  3. Arnhold SJ, Goletz I, Klein H, Stumpf G, Beluche LA, Rohde C, et al. Isolation and characterization of bone marrow-derived equine mesenchymal stem cells. Am J Vet Res. 2007; 68(10): 1095-1105.
  1. Friedenstein AJ, Chailakhjan RK, Lalykina KS. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 1970; 3(4): 393-403.
  2. Khojasteh A, Eslaminejad MB, Nazarian H. Mesenchymal stem cells enhance bone regeneration in rat calvarial critical size defects more than platelete-rich plasma. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008; 106(3): 356-362.
  3. Ravichandran R, Liao S, Ng C, Chan CK, Raghunath M, Ramakrishna S. Effects of nanotopography on stem cell phenotypes. World J Stem Cells. 2009; 1(1): 55-66.
  4. Jones N, Ray B, Ranjit KT, Manna AC. Antibacterial activity of ZnO nanoparticle suspensions on a broad spectrum of microorganisms. FEMS Microbiol Lett. 2008; 279(1): 71–76.
  5. Hanley C, Thurber A, Hanna C, Punnoose A, Zhang J, Wingett DG. The influences of cell type and ZnO nanoparticle size on immune cell cytotoxicity and cytokine induction. Nanoscale Res Lett. 2009; 4 (12): 1409–1420.