Interaction between anxiolytic effects of magnesium oxide nanoparticles and exercise in adult male rat

Editorial

Authors

1 Department of Biology, Faculty of Science, Shahid Chamran University, Ahvaz, Iran

2 Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Shahid Chamran University, Ahvaz, Iran

Abstract

Objective(s):
In recent years, nanotechnology has produced new forms of materials that are more effective than their predecessors. Magnesium is an essential element in the human body and certain studies have proved that its deficiency can induce anxiety in animals. In this study, the effect of magnesium oxide nanoparticles (MgO NPs) on anxiety, related behaviors, and interaction between their effects and anxiolytic effect of the exercises were examined in comparison to the conventional MgO (cMgO).
Materials and Methods: 
Adult male Wistar rats weighing 190±20 gr were divided into control groups (receiving saline, without physical activity), and exercise groups (receiving cMgO and/or MgO NPs (1 mg/kg i.p.) daily for 6 weeks with or/and without exercise). Exercise groups were performing their daily physical activity protocol 30 minutes after injection. At the end of period, an elevated plus maze apparatus was used to evaluate the anxiety (%pen arm time (%OAT) and %open arm entries (%OAE) and locomotor activity.
Results:
Exercise significantly increased %OAT and %OAE (P<0.05). MgO NPs caused an increase in %OAT, while cMgO did not have any effect on %OAT or %OAE. There was no notable difference among anxiety parameters in exercise groups with or without taking MgO NPs.
Conclusion:
It seems that the anxiolytic effect of exercise and MgO NPs has been mediated through common mechanisms that were a part of the anxiety process of the central nervous system.

Keywords


  1. Lukaski HC. Magnesium, zinc, and chromium nutriture and physical activity. Am J Clin Nutr. 2000; 72(2): 585S-593S.
  2. Poleszak E, Szewczyk B, Kedzierska E, Wlaź P, Pilc A, Nowak G.   Antidepressant and anxiolytic-like activity of magnesium in mice. Pharmacol Biochem Behav. 2004; 78(1): 7-12.
  3. Sartori SBWhittle NHetzenauer ASingewald N. Magnesium deficiency induces anxiety and HPA axis dysregulation: modulation by therapeutic drug treatment. Neuropharmacology. 2012; 62(1): 304-312.
  4. Uteva AG, Pimenov LT. Magnesium deficiency and anxiety-depressive syndrome in elderly patients with chronic heart failure. Adv Gerontol. 2012; 25(3): 427-432. [Article in Russian].
  5. Hasanein P, Parviz M, Keshavarz M, Javanmardi K, Allahtavakoli M, Ghaseminejad M. Modulation of cholestasis-induced antinociception in rats by two NMDA receptor antagonists: MK-801 and magnesium sulfate. Eur J Pharmacol. 2007; 554 (2-3): 123-127.
  6. Gerstein M, Huleihel M, Mane R, Stilman M, Kashtuzki I, Hallak M, et al. Remodeling of hippocampal GABAergic system in adult offspring after maternal hypoxia and magnesium sulfate load: Immunohistochemical study.  Exp Neurol. 2005; 196 (1): 18 – 29.
  7. Poleszak E. Benzodiazepine/GABA (A) receptors are involved in magnesium-induced anxiolytic-like behavior in mice. Pharmacol Rep. 2008; 60(4): 483-489.
  8. Heidary A, Emami A, Eskandaripour S, Saiah A, Hamidi S, Shahbazi M. Effects of aerobic exercise on anxiety. Procedia Soc Behav Sci. 2011; 30: 2497-2498.
  9. Pietrelli A, Lopez-Costa J, Goni R, Brusco A, Basso N. Aerobic exercise prevents age-dependent cognitive decline and reduces anxiety-related behaviors in middle-aged and old rats. Neuroscience. 2012; 202: 252-266.
  10. Binder E, Droste SK, Ohl F, Reul JMHM. Regular voluntary exercise reduces anxiety-related behaviour and impulsiveness in mice. Behav Brain Res. 2004; 155(2): 197-206.
  11. Vollert C, Zagaar M, Hovatta I, Taneja M, Vu A, Dao A, et al. Exercise prevents sleep deprivation-associated anxiety-like behavior in rats: Potential role of oxidative stress mechanisms. Behav Brain Res. 2011; 224(2): 233-240.
  12. Herring MP, Jacob ML, Suveg C, O’Connor PJ. Effects of short-term exercise training on signs and symptoms of generalized anxiety disorder. Ment Health Phys Act. 2011; 4(2): 71-77.
  13. Hsu MH, Wang JM, Lee MS, Lee CP, Cheng FC, Lin MT, et al. Changes in serum magnesium concentration in trained and untrained subjects after exercise. J Biomed Lab Sci. 2007; 19(1): 25-29.
  14. Cheng SM, Yang LL, Chen SH, Hsu MH, Chen IJ, Cheng FC. Magnesium sulfate enhances exercise performance and manipulates dynamic changes in peripheral glucose utilization.  Eur J Appl Physiol. 2010; 108(2): 363-369.
  15. Nunes A,  Al-Jamal KT, Kostarelos K. Therapeutics, imaging  and toxicity of nanomaterials in the central nervous system. J Control Release. 2012; 161(2):  290-306.
  16. Kreuter J. Application of nanoparticles for the delivery of drugs to the brain. Int Congr Ser. 2005; 1277: 85-94.
  17. Cho Y, Borgens RB. Polymer and nano-technology applications for repair and reconstruction of the central nervous system.  Exp Neurol. 2012; 233: 126-144.
  18. Sonavane G, Tomoda K, Makino K. Biodistribution of colloidal gold nanoparticles after intravenous administration: effect of particle size.  Colloids Surf B Biointerfaces. 2008; 66(2): 274-280.
  19. Garnett MC, Kallinteri P. Nanomedicines and nanotoxicology: some physiological principles.  Occup Med (Lond). 2006; 56(5): 307-311.
  20. Sekhon BS,  Kamboj SR. Inorganic nanomedicine—Part1. Nanomedicine.  2010; 6(4): 516-522.  
  21. Alexa IF, Ignat M, Popovici RF, Timpu D, Popovici E. In vitro controlled release of antihypertensive drugs intercalated into unmodified SBA-15 and MgO modified SBA-15 matrices. Int J Pharm. 2012; 436(1-2): 111-119.
  22. Kesmati M, Torabi M, Malekshahi Nia H, Teymuri Zamaneh H. Effect of chronic administration of zinc supplements (ZnO and nano ZnO) with and without aerobic exercise on nociception in male rats.  Physiol Pharmacol. 2013; 16 (4): 415-422. [Article in Persian].
  23. Ashabi G, Oryan S, Ahmadi R, Valizadegan F. The effects of hippocampal opioidergic and septal GABAergic system interactions on anxiety-like behavior in rats. Life Sci. 2011; 89(21-22): 821-826.
  24. Pietropaolo S, Sun Y, Li R, Brana C, Feldon J, Yee BK. The impact of voluntary exercise on mental health in rodents: a neuroplasticity perspective. Behav Brain Res. 2008; 192(1): 42-60.
  25. Salmon P. Effects of physical exercise on anxiety, depression, and sensitivity to stress: A unifying theory. Clin Psychol Rev. 2001; 21(1): 33-61.
  26. Mandroukas K, Zakas A, Aggelopoulou N, Christoulas K, Abatzides G, Karamouzis M. Atrial natriuretic factor responses to submaximal and maximal exercise. Br J Sports Med. 1995; 29(4): 248-251.
  27. Ströhle A. Physical activity, exercise, depression and anxiety disorders.  J Neural Transm. 2009; 116(6): 777-784.
  28. Koltyn KF. Analgesia following exercise, a review. Sports Med. 2000; 29: 85-98.
  29. Singewald N, Sinner C, Hetzenauer A, Sartori SB, Murck H. Magnesium-deficient diet alters depression- and anxiety-related behavior in mice influence of desipramine and Hypericum perforatum extract. Neuropharmacology. 2004; 47(8): 1189-1197.
  30. Bergink V, Van Megen HJGM, Westenberg HGM. Glutamate and anxiety.  Eur J Neuropsychopharmacol. 2004; 14(3): 175-183.
  31. Xie Z, Commissaris L. Anxiolytic-like effects of the noncompetitive NMDA antagonist MK801. Pharmacol Biochem Behav. 1992; 43(2): 471-477.
  32. Arora S, Rajwade JM, Paknikar KM. Nanotoxicology and in vitro studies: The need of the hour. Toxicol Appl Pharmacol. 2012; 258(2): 151-165.
  33. Di DR, He ZZ, Sun ZQ, Liu J. A new nano-cryosurgical modality for tumor treatment using biodegradable MgO nanoparticles. Nanomedicine. 2012; 8(8): 1233-1241.
  34. Goodwin ML,  Harris JE, Hernández A,  Gladden LB. Blood Lactate Measurements and Analysis during Exercise: A Guide for Clinicians. J Diabetes Sci Technol. 2007; 1(4): 558-569.
  35. Mooren FC, Golf SW, Lechtermann A, Völker K.  Alterations of ionized Mg2+ in human blood after exercise. Life Sci. 2005; 77: 1211-1225.