Preparation and investigation of polylactic acid, calcium carbonate and polyvinylalcohol nanofibrous scaffolds for osteogenic differentiation of mesenchymal stem cells

Document Type: Research Paper


1 Department of chemical engineering, University of Zanjan, Zanjan, Iran

2 Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran



Objective(s): In this study, the effect of electrospun fiber orientation on proliferation and differentiation of mesenchymal stem cells (MSCs) was evaluated.
Materials and Methods: Aligned and random nanocomposite nanofibrous scaffolds were electrospun from polylactic acid (PLA), poly (vinyl alcohol) (PVA) and calcium carbonate nanoparticles (nCaP). The surface morphology of prepared nanofibrous scaffolds with and without cell was examined using scanning electron microscopy. Mechanical properties of electrospun nanofibrous scaffolds were determined with a  universal testing machine. The in vitro properties of fabricated scaffolds was also investigated by the MTT assay and alkaline phosphatase activity (ALP).
Results: The average fiber diameter for aligned and random nanofibers were 82 ± 12 nm and 124 ± 25 nm, respectively. The mechanical testing indicated the higher tensile strength and elastic modulus of aligned nanofibers. MTT and ALP results showed that alignment of nanofiber increased the osteogenic differentiation of stem cells.
Conclusion: Aligned nanofibrous nanocomposite scaffolds of PLA/nCaP/PVA could be an excellent substrate for MSCs and represents a potential bone-filling material.


[1]  Lyu S, Huang C, Yang H, Zhang X. Electrospun Fibers as a Scaffolding Platform for Bone Tissue Repair. J Orthop Res. 2013; 31: 1382–1389.

[2]  Lee IS, Kwon OH, Meng W, Kang IK. Nanofabrication of microbial polyester by electrospinning promotes cell attachment. Macromol Res. 2004; 12: 374-378.

[3]  Khadka DB, Haynie DT. Protein and peptide-based elect- rospun nanofibers in medical biomaterials. Nanomedicine. 2012; 8: 1242-1262.

[4]  Subramanian A, Krishnan UM, Sethuraman S. Development of biomaterial scaffold for nerve tissue engineering: Biomaterial mediated neural regeneration. J Biomed Sci. 2009; 16: 108.

[5]  Gomez-Tejedor JA, Van Overberghe N, Rico P, Ribelles JLG. Assessment of the parameters influencing the fiber characteristics of electrospun poly(ethyl methacrylate) membranes. Eur Polym J. 2011; 47: 119-129.

[6]  Jayakumar R, Prabaharan M, Shalumon KT, Chennazhi KP, Nair SV. Biomedical Applications of Polymer/Silver Composite Nanofibers. Berlin: Springer-Verlag; 2012.

[7]  Kenawy ER, Bowlin GL, Mansfield K, Layman J, Simpson DG, Sanders EH, Wnek GE. Release of tetracycline hydrochloride from electrospun poly(ethylene-co-vinylacetate), poly(lactic acid), and a blend. J Control Release. 2002; 81: 57-64.

[8]  Min BM, Jeong L, Nam YS, Kim JM, Park WH.  Formation of silk fibroin matrices with different texture and its cellular response to normal human keratinocytes. Int J Biol Macromol. 2004; 34: 281-288.

[9]  Yoshimoto H, Shin YM, Terai H, Vacanti JP. A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering. Biomaterials. 2003; 24: 2077-2082.

[10] Li WJ, Tuli R, Okafor C, Derfoul A, Danielson KG, Hall DJ, Tuan RS. A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells. Biomaterials. 2005; 26: 599-609.

[11] Reneker DH, Chun I. Nanometre diameter fibres of   polymer, produced by electrospinning. Nanotechnology.

       1996; 7: 216-223.

[12] Li D, Xia Y. Electrospinning of Nanofibers: Reinventing the Wheel?. Adv Mater. 2004; 16: 1151-1170.

[13] Fujihara K, Kotaki M, Ramakrishna S. Guided bone regeneration membrane made of  polycaprolactone/calcium carbonate composite nano-fibers. Biomaterials 2005;  26: 4139-4147.

[14] Turhani D, Cvikl B, Watzinger E, Weizenhock M, Yerit K, Thurnher D, Lauer G, Ewers R, In Vitro Growth an Differentiation of Osteoblast-Like Cells on Hydroxyapatite Ceramic Granule Calcified From Red Algae. Oral Maxillofacial Surg. 2005; 63: 793-799.

[15] Coombes AG, Rizzi SC, Williamson M, Barralet JE, Downes S, Wallace WA. Precipitation casting of polycaprolactone for applications in tissue engineering and drug delivery. Biomaterials. 2004; 25: 315-325.

[16] Maeno S, Niki Y, Matsumoto H, Morioka H, Yatabe T, Funayama A, Toyama Y, Taguchi T, Tanaka J. The effect of calcium ion concentration on osteoblast viability, proliferation and differentiation in monolayer and 3D culture. Biomaterials. 2005; 26: 4847-4855.

[17] Ma J, He X, Jabbari E. Osteogenic Differentiation of Marrow Stromal Cells on Random and Aligned Electrospun Poly (l-lactide) Nanofibers. Annals biomed Eng. 2011; 39: 14-25.

[18] Schnell E, Klinkhammer K, Balzer S, Brook G, Klee D, Dalton P, Mey J. Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-epsilon-caprolactone and a collagen/polycaprolactone blend. Biomaterials. 2007; 28(19): 3012-3025.

[19] Horwitz EM, Le Blanc K, Dominici M, Mueller I, Slaper-Cortenbach I, Marini FC, et al. Clariûcation of the nomenclature for MSC: the International Society for cellular therapy position statement. Cytotherapy. 2005; 7(5): 393-395.

[20] Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, et al. Minimal criteria for deûning multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement Cytotherapy. 2006; 8(4): 315-317.

[21] Pittenger MF. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;  284(5411): 143-147.

[22] Prockop DJ. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science. 1997; 276(5309): 71-74.

[23] Vinatier C, Mrugala D, Jorgensen C, Guicheux J, Noel D. Cartilage engineering: a crucial combination of cells, biomaterials and biofactors. Trends Biotechnol. 2009; 27(5): 307-314.

[24] Li W-J, Tuli R, Huang X, Laquerriere P, Tuan RS. Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanoûbrous scaffold. Biomaterials. 2005; 26(25): 5158-5166.

[25]  Karp JM, Leng Teo GS. Mesenchymal stem cell homing: the devil is in the details. Cell Stem Cell. 2009; 4(3): 206-216.

[26] Yin Z, Chen X, Chen JL, Shen WL, Nguyen TMH, Gao L, Ouyang HW. The regulation of  tendon stem cell differentiation by the alignment of nanofibers. Biomaterials. 2010; 31: 2163-2175.