Evaluation the effect of silver nanoparticles on oxidative stress biomarkers in blood serum and liver and kidney tissues

Document Type: Research Paper

Authors

Department of Biology, Division of Animal Physiology, Faculty of Basic Sciences, Shahrekord University, Shahrekord, Iran

10.7508/nmj.2016.03.005

Abstract

Objective(s): Silver nanoparticles (Ag-NPs) are one of the most widely used nanomaterials recently. Despite the wide application of nanomaterials, there is limited information concerning their impact on human health and the environment. This study aimed to find the effects of Ag-NPs (40 nm) on blood serum, liver and kidney tissues of homing pigeons (Columbia livia).
Materials and Methods: Columba livia, in vivo model used in ecotoxicity experiments were gavaged 3 times daily with 75 and 150 ppm of Ag-NPs within 14 days. A group of 30 Pigeon were randomly divided into three groups: Ag-NPs exposed and control groups (n=10). Data analysis was counducted by performing one-way variance (ANOVA) in SPSS.v.16.0                                                                         
Results: The results of this study illustrated that in the enzyme activity of Glutathione S –transferase (GST), Aspartate amino transferase (AST), Alanine amino transferase (ALT) and lactate dehydrogenas (LDH) there is a significant difference between treatment groups with Ag-NPs and the control group. Also, lipid peroxidation (LPO) analysis and catalase activity CAT) suggest Ag-NPs cause the main damage to the liver tissue. On the other hand: Ag-NPs have toxic and harmful effects in both concentrations (75 and 150 ppm), and cause LPO induction, oxidative stress and increase of biomarkers of liver necrosis in under treatment pigeons.
Conclusion: The results of this study show that the organism’s exposure to Ag-NPs cause toxicity that is dose-dependant. in this study, the highest damage was observed in the liver. However, this issue will have to be considered more extensively in further studies.   

Keywords


[1] Bilberg K, Hovgaard MB, Besenbacher F, Baatrup E. In Vivo Toxicity of Silver Nanoparticles and Silver Ions in Zebrafish (Danio rerio). J Toxicol. 2011; 2012: 15-24.

[2] Asghari S, Johari SA, Lee JH, Kim YS, Jeon YB, Choi HJ, Moon MC,Yu JI. Toxicity of various silver nanoparticles compared to silver ions in Daphnia magna. J Nanobiotechnology. 2012; 10(2): 3-14.

[3] Choi O, Deng KK, Kim NJ, Ross L, Surampalli RY, Hu Z. The inhibitory effects of silver nanoparticles, silver ions, and silver chloride colloids on microbial growth. Water Res. 2008; 42(12): 3066-3074.

[4] Mcneil SE. Nanotechnology for the biologist. J Leukoc Biol. 2005; 78(3): 585-595.

[5] Farkas J, Christian P, Urrea JAG, Roos N, Hassellov M, Tollefsen KE,Thomas KV. Effects of silver and gold nanoparticles on rainbow trout (Oncorhynchusmykiss) hepatocytes. Aqua Toxicol. 2010; 96(1): 44-52.

[6] Kenawy ER, Worley SD, Broughton R. The chemistry and applications of antimicrobial polymers: a state-of-the-art review. Bio macromolecules. 2007; 8(5): 1359-1384.

[7] Costantini D. Complex trade-offs in the pigeon (Columba livia): egg antioxidant capacity and female serum oxidative status in relation to diet quality. J Comp Physiol B. 2010; 180(5): 731-739.

[8] Huang YW, Wu C, Aronstam RS. Toxicity of Transition Metal Oxide Nanoparticles: Recent Insights from in vitro Studies. Int J Mol Sci. 2010; 3(10): 4842-4859.

[9] Kim S, Choi JE, Choi J, Chung KH. Park K, Yi J, Ryu DY. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. In Vitro Toxicol. 2009; 23(6): 1076-1084.

[10] Negahdary M,  Chelongar R,  Kabiri Zadeh Sh,  Ajdary M. The antioxidant effects of silver, gold, and zinc oxide nanoparticles on male mice in vivo condition. Adv Biomed Res. 2015; 4: 69-81.

[11] Buege JA, Aust SD. Microsomal lipid peroxidation. Method enzymol. 1978; 52: 302-318.

[12] Aebi H, Wyss SR, Scherz B, Skvaril F. Heterogeneity of Erythrocyte Catalase II. Eur J Biochem. 1974; 48(1): 137-145.

[13] Benson AM, Cha YN, Bueding E, Heine HS, Talalay P. Elevation of extrahepatic glutathione S-transferase and epoxide hydratase activities by 2 (3)-tert-butyl-4-hydroxyanisole. Cancer Res. 1979; 39(8): 2971-2978.

[14] Wootton IDP, King EJ, Freeman H. Microanalysis in medical biochemistry. Edinburgh: Churchill Livingstone. 6th ed. 1982.

[15] Markwell MA, Haas SM, Bieber LL, Tolbert NE. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978; 87(1): 206-210.

[16] Tang J, Xiong L, Wang S, Wang J, Liu L, Li J, Fuqiang Y, Tingfei X. Distribution, translocation and accumulation of silver nanoparticles in rats. J Nanosci Nanotechnol. 2009; 9(8): 4924-4932.

[17] Kim S, Ryu DY. Silver nanoparticle induced oxidative stress, genotoxicity and apoptosis in cultured cells and animal tissues. J Appl Toxicol. 2013; 33(2):78-98.

[18] Schrand AM, Braydich-Stolle LK, Schlager JJ, Dai L, Hussain SM. Can silver nanoparticles be useful as potential biological labels? Nanotechnology. 2008; 19(23): 35-256.

[19] Scown TM, Santos EM, Johnston BD, Gaiser B, Baalousha M, Mitov S, Lead JR, Stone V, Fernandes TF, Jepson M, Aerle AV, Tyler CA. Effects of aqueous exposure to silver nanoparticles of different sizes in rainbow trout. Toxicol Sci. 2010; 115(2): 521-534.

[20] Ahamed M, Posgai R, Gorey TJ, Nielsen M, Hussain SM, Rowe JJ. Silver nanoparticles induced heat shock protein 70, oxidative stress and apoptosis in Drosophila melanogaster. Toxicol Applied pharmacol. 2010; 242(3): 263-269.

[21] Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. In Vitro Toxicol. 2005; 19(7): 975-983.

[22] Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC. In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicol Sci. 2005; 88(2): 412-419.

[23] Tiwari DK, Jin T, Behari J. Dose-dependent in-vivo toxicity assessment of silver nanoparticle in Wistar rats. Toxicol Mech Methods. 2011; 21(1): 13-24.

[24] Madurawe RD, Lin Z, Dryden PK, Lumpkin JA. Stability of Lactate Dehydrogenase in Metal Catalyzed Oxidation Solutions Containing Chelated Metals. Biotechnol Prog. 1997; 13(2): 179-184.

[25] Schrand AM, Rahman MF, Hussain SM, Schlager JJ, Smith DA, Syed AF. Metal based nanoparticles and their toxicity assessment.Wiley Interdiscip Rev Nanomed Nanobiotecnol. 2010; 2(5): 544-568.

[26] Breccia JD, Andersson MM, Hatti-Kaul R. The role of poly (ethyleneimine) in stabilization against metal-catalyzed oxidation of proteins: a case study with lactate dehydrogenase. Biochim Biophys Acta. 2002; 1570(3): 165-173