Investigation of drug release from paclitaxel loaded polylactic acid nanofibers

Document Type: Research Paper

Author

Department of chemical engineering, University of Zanjan, Zanjan, Iran

10.7508/nmj.2016.03.006

Abstract

Objective(s): In this study, drug loaded electrospun nanofibrous mats were prepared and drug release and mechanism from prepared nanofibers were investigated.  
Materials and Methods: Paclitaxel (PTX) loaded polylactic acid (PLA) nanofibers were prepared by electrospinning. The effects of process parameters, such as PTX concentration, tip to collector distance, voltage, temperature and flow rate on the mean diameter of electrospun PTX loaded PLA nanofibers were investigated. Scanning electron microscopy (SEM) was used to investigate the fiber morphology and mean fiber diameter of prepared nanofibers. Response surface methodology was used to model the average diameter of electrospun PLA/PTX nanofibers.
Results: The predicted fiber diameter was in good agreement with the experimental result. In Vitro drug release in phosphate buffer solution (PBS) and acetate buffer for the produced samples showed that diffusion is the dominant drug release mechanism for PTX loaded ubers.
Conclusion: Electrospinning was shown to be very promising approach to the formulation of Paclitaxel in order to enhance its release in a sustained and prolonged manner.

Keywords


[1] Zeng  J,  Xu  X,  Chen  X,  et  al.  Biodegradable  electrospun  fibers  for  drug  delivery. J Control Release. 2003; 92: 227–231.

[2] Altman GH, Diaz F, Jakuba C, et al. Silk-based biomaterials. Biomaterials. 2003; 24: 401–416.

[3] Chen JP, Ho KH, Chiang YP, et al. Fabrication of electrospun poly(methyl methacrylate) nanofibrous membranes by statistical approach for application in enzyme immobilization. J Mem Sci. 2009; 340: 9–15.

[4] Heikkila¨ P , Harlin  A. Parameter  study of electrospinning  of  polyamide-6. Eur Polym J. 2008; 44: 3067–3079.

[5] Gu SY, Ren J and Vancso GJ. Process optimization and empirical modeling for electrospun polyacrylonitrile (PAN) nanofiber precursor of carbon nanofibers. Eur Polym J. 2005; 41: 2559–2568.

[6] Yordem OS, Papila M and Menceloglu YZ. Effects of electrospinning parameters on polyacrylonitrile nanofiber diameter: An investigation  by response surface methodology. Mater Des. 2008; 29: 34–44.

[7] Sukigara  S,  Gandhi  M,  Ayutsede  J,  et  al.  Regeneration  of  Bombyx  mori  silk  by electrospinning. Part 2. Process optimization and empirical modeling using response surface methodology. Polymer. 2004; 45: 3701–3708.

[8] Zahedi P, Rezaeian I, Jafari SH, et al. Preparation and release properties of electrospun poly(vinyl alcohol)/poly([-caprolactone)  hybrid  nanofibers:  Optimization  of  process parameters via D-optimal design method. Macromol Res. 2013; 21: 649–659.

[9] Sill TJ and Von Recum HA. Electro spinning: Applications in drug delivery and tissue engineering. Biomaterials. 2008; 29: 1989–2006.

[10] Liang  D  and  Chu  B.  Functional  electrospun  nanofibrous  scaffolds  for  biomedical applications. Adv Drug Deliv Rev. 2007; 59: 1392–1412.

[11] Jeong B, Bae YH, Lee DS, et al. Biodegradable block copolymers as injectable drug delivery systems. Nature. 1997; 388: 860–862.

[12] Jin  HJ,  Fridrikh  SV,  Rutledge  GC,  et  al.  Electrospinning  Bombyx  mori  silk  with poly (ethylene oxide). Biomacromolecules. 2002; 3: 1233–1239.

[13] Okuda  T,  Tominaga  K  and  Kidoaki  S.  Time-programmed  dual  release  formulation  by  multilayered  drug-loaded  nanofiber  meshes.  J  Control  Release.  2010;  143: 258–264.

[14] Korsmeyer RW, Gurny R, Doelker E, et al. Mechanisms of solute release from porous hydraulic polymers. Int J Pharm. 1983; 15: 25–35.

[15] Peppas NA. Analysis of Fickian and non-Fickian drug release from polymers. Pharm Acta Helv. 1985; 60: 110–111.

[16] Xu X, Chen X, Wang Z, et al. Ultrafine PEG-PLA fibers loaded with both paclitaxel and doxorubicin hydrochloride and their in vitro cytotoxicity. Eur J Pharm Biopharm. 2009; 72: 18–25.