The effect of electrospun poly(lactic acid) and nanohydroxyapatite nanofibers’ diameter on proliferation and differentiation of mesenchymal stem cells

Document Type : Research Paper

Author

Department of chemical engineering, University of Zanjan, Zanjan, Iran

Abstract

Objective(s): Electrospun nanofibrous mats of poly(lactic acid) (PLA) and nanohydroxyapatite (nano-HA) were prepared and proliferation and differentiation of mesenchymal stem cells on the prepared nanofibers were investigated in this study.
Materials and Methods: PLA/nano-HA nanofibers were prepared by electrospinning. The effects of process parameters, such as nano-HA concentration, distance, applied voltage, and flow rate on the mean diameter of electrospun nanofibers were investigated. Scanning electron microscopy (SEM) was used to determine the mean fiber diameter of produced nanofibers. Mechanical propertes of nanofibrous mats were evaluated using a universal testing machine. Response surface methodology was used to model the fiber diameter of electrospun PLA/nano-HA nanofibers.
Results: The average fiber diameter for optimized nanofibers was 125 ± 11 nm. MTT and ALP results showed that optimization of fiber diameter increased the osteogenic differentiation of stem cells.
Conclusion: It could be concluded that optimization of fiber diameter has beneficial effect on cell proliferation and differentiation. Optimized nanofibers of PLA/nano-HA could be good candidates for bone tissue engineering.

Keywords


1. Liao S, Chan CK, Ramakrishna S. Stem cells and biomimetic materials strategies for tissue engineering. Mater Sci Eng C. 2008; 28: 1189-1202.
2. Araujo JV, Martins A, Leonor IB, Pinho ED, Reis RL, Neves NM. Surface controlled biomimetic coating of polycaprolactone nanofiber meshes to be used as bone extracellular matrix analogues. J Biomater Sci Polym Ed. 2008; 19: 1261-1278.
3. Liu Y, Wang G, Cai Y, Ji H, Zhou G, Zhao X, Tang R, Zhang M. In Vitro effect of nanophase hydroxyapatite particles on proliferation and osteogenic differentiation of bone marrow derived mesenchymal stem cells. J Biomed Mater Res. 2009; 90A: 1083-1091.
4. Horwitz EM, Le Blanc K, Dominici M, Mueller I, Slaper-Cortenbach I, Marini FC, Deans RJ, Krause DS, Keating A. Clarification of the nomenclature for MSC: the International Society for cellular therapy position statement. Cytotherapy. 2005; 7(5): 393-395.
5. Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj, Horwitz E. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy. 2006; 8(4): 315-317.
6. Pittenger MF. Multilineage potential of adult human mesenchymal stem cells. Science. 1999; 284 (5411): 143-147.
7. Prockop DJ. Marrow stromal cells as stem cells for nonhematopoietic tissues. Science. 1997; 276 (5309): 71-74.
8. Vinatier C, Mrugala D, Jorgensen C, Guicheux J, Noël D. Cartilage engineering: a crucial combination of cells, biomaterials and biofactors. Trends Biotechnol. 2009; 27(5): 307-314.
9. Li W-J, Tuli R, Huang X, Laquerriere P, Tuan RS. Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials. 2005; 26(25): 5158-5166.
10. Karp JM, Leng Teo GS. Mesenchymal stem cell homing: the devil is in the details. Cell Stem Cell. 2009; 4(3): 206-216.
11. Sundaray B, Subramanian V, Natarajan TS, Xiang RZ, Chang CC, Fann WS. Electrospinning of continuous aligned polymer fibers. Appl Phys Lett. 2004; 84: 1222-1224.
12. Huang ZM, Zhang YZ, Kotaki M, Ramakrishna S. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Compos Sci Technol. 2003; 63: 2223-2253.
13. Matthews JA, Boland ED, Wnek GE, Simpson DG, Bowlin GL. Electrospinning of collagen type II: A feasibility study. J Bioact Compat Polym. 2003; 18: 125-134.
14. Kidoaki S, Kwon IK, Matsuda T. Mesoscopic spatial designs of nano- and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques. Biomaterials. 2005; 26: 37-46.
15. Min BM, Lee G, Kim SH, Nam YS, Lee TS, Park WH. Electrospinning of silk fibroin nanofibers and its effect on the adhesion and spreading of normal human keratinocytes and fibroblasts in vitro. Biomaterials. 2004; 25(7/8): 1289-1297.
16. Stamatialis DF, Papenburg BJ, Giron´es M, Saiful S. Medical applications of membranes: Drug delivery, artificial organs and tissue engineering. J Membr Sci. 308 (2008) 1-34.
17. Fabbri P, Bondioli F, Messori M, Bartoli C, Dinucci D, Chiellini F. Porous scaffolds of polycaprolactone reinforced with in situ generated hydroxyapatite for bone tissue engineering. J Mater Sci Mater Med. 2010; 21: 343-351.
18. Kwon IK, Kidoaki S, Matsuda T. Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential. Biomaterial. 2004; 26: 3929-3939.
19. Acatay K, Simsek E, Yang CO, Mencelo YZ. Tunable, Superhydrophobically Stable Polymeric Surfaces by Electrospinning. Angew Chem Int Ed. 2004; 43: 5210-5213.
20. Baji A, Mai YW, Wong SC. Electrospinning of polymer nanofibers: effects on oriented morphology, structures and tensile properties. Compos Sci Technol. 2010; 70: 703-718.
21. Yang F, Murugan R, Wang S, Ramakrishna S. Electrospinning of nano/micro scale polyL-lactic acid aligned fibers and their potential in neural tissue engineering. Biomaterials. 2005; 26: 2603-2610.