Mycosynthesis and characterization of selenium nanoparticles using standard penicillium chrysogenum PTCC 5031 and their antibacterial activity: A novel approach in microbial nanotechnology

Document Type : Research Paper


1 Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Department of Medicinal Chemistry, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Student Research Committee, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Department of Pharmaceutics, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5 Department of Medical Microbiology and Immunology, Division of Biomedical Sciences, School of Medicine, College of Health Sciences, Mekelle University, Mekelle, Ethiopia


Objective(s): This study deals with mycosynthesis and characterization of selenium nanoparticles (SeNPs) using the Penicillium chrysogenum PTCC 5031 and evaluating their antibacterial activity.
Materials and Methods: The formation of SeNPs was confirmed with the color change from pale yellow to orange. Tyndall effect also confirmed the formation of colloidal systems through the samples. The SeNPs were characterized using different analytical techniques including photon correlation spectroscopy (PCS), Scanning Electron Microscope (SEM), Atomic Force Microscope (AFM), Energy Dispersive X-ray (EDX), X-ray diffraction (XRD) and Fourier Transform Infrared (FT-IR) analysis.
Results: Our findings revealed that SeNPs were fairly uniformed with good monodispersity and the lesser aggregation of particles in pH value of 7 with the average hydrodynamic size of 24.65 nm, polydispersity index (PdI) of 0.392 and zeta potential of -34 mV. The SeNPs revealed antibacterial activity against gram positive bacteria including Staphylococcus aureus, and Listeria monocytogenes with the zone of inhibition (ZOI) of 10 and 13 mm, respectively.
Conclusion: The results of this study provided a potential solution to the growing need for the development of cost-effective and eco-friendly ways of nanoparticle synthesis to overcome the microbial resistance and control the infectious diseases. However, further investigations are required to demonstrate the efficacy of SeNPs through in vivo models.


1.Morais MGd, Martins VG, Steffens D, Pranke P, da Costa JAV. Biological applications of nanobiotechnology. J Nanosci. 2014; 14(1): 1007-1017.
2.Fakruddin M, Hossain Z, Afroz H. Prospects and applications of nanobiotechnology: a medical perspective. J Nanobiotechnol. 2012; 10(1): 1-8.
3.Barabadi H, Honary S. Biofabrication of gold and silver nanoparticles for pharmaceutical applications. Pharm Biomed Res. 2016; 2(1): 1-7.
4.Saravanan M, Vahidi H, Cruz DM, Vernet-Crua A, Mostafavi E, Stelmach R, Webster TJ, Mahjoub MA, Rashedi M, Barabadi H. Emerging antineoplastic biogenic gold nanomaterials for breast cancer therapeutics: a systematic review. Int J Nanomed. 2020; 15: 3577-3595.
5.Yaqoob SB, Adnan R, Rameez Khan RM, Rashid M. Gold, silver, and palladium nanoparticles: a chemical tool for biomedical applications. Front Chem. 2020; 8: 376.
6.Pugazhendhi A, Edison TNJI, Karuppusamy I, Kathirvel B. Inorganic nanoparticles: A potential cancer therapy for human welfare. Int J Pharm. 2018; 539(1): 104-111.
7.Anu K, Devanesan S, Prasanth R, AlSalhi MS, Ajithkumar S, Singaravelu G. Biogenesis of selenium nanoparticles and their anti-leukemia activity. J King Saud Univ, Sci. 2020; 32(4): 2520-2526.
8.Menon S, Agarwal H, Rajeshkumar S, Jacquline Rosy P, Shanmugam VK. Investigating the antimicrobial activities of the biosynthesized selenium nanoparticles and its statistical analysis. BioNanoScience. 2020; 10(1): 122-135.
9.Shanmuganathan R, Sathishkumar G, Brindhadevi K, Pugazhendhi A. Fabrication of naringenin functionalized-Ag/RGO nanocomposites for potential bactericidal effects. J Mater Res Technol. 2020; 9(4): 7013-7019.
10.Samuel MS, Selvarajan E, Mathimani T, Santhanam N, Phuong TN, Brindhadevi K, Pugazhendhi A. Green synthesis of cobalt-oxide nanoparticle using jumbo Muscadine (Vitis rotundifolia): Characterization and photo-catalytic activity of acid Blue-74. J Photochem Photobiol, B. 2020; 211: 112011.
11.Munawer U, Raghavendra VB, Ningaraju S, Krishna KL, Ghosh AR, Melappa G, Pugazhendhi A. Biofabrication of gold nanoparticles mediated by the endophytic Cladosporium species: Photodegradation, in vitro anticancer activity and in vivo antitumor studies. Int J Pharm. 2020; 588: 119729.
12.Govindaraju K, Vasantharaja R, Suganya KU, Anbarasu S, Revathy K, Pugazhendhi A, Karthickeyan D, Singaravelu G. Unveiling the anticancer and antimycobacterial potentials of bioengineered gold nanoparticles. Process Biochem. 2020; 96: 213-219.
13.Sisubalan N, Ramkumar VS, Pugazhendhi A, Karthikeyan C, Indira K, Gopinath K, Hameed AS, Basha MH. ROS-mediated cytotoxic activity of ZnO and CeO2 nanoparticles synthesized using the Rubia cordifolia L. leaf extract on MG-63 human osteosarcoma cell lines. Environ Sci Pollut Res. 2018; 25(11): 10482-10492.
14.Shanmuganathan R, MubarakAli D, Prabakar D, Muthukumar H, Thajuddin N, Kumar SS, Pugazhendhi A. An enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach. Environ Sci Pollut Res. 2018; 25(11): 10362-10370.
15.Zahin N, Anwar R, Tewari D, Kabir MT, Sajid A, Mathew B, Uddin MS, Aleya L, Abdel-Daim MM. Nanoparticles and its biomedical applications in health and diseases: special focus on drug delivery. Environ Sci Pollut Res. 2020; 27(16): 19151-19168.
16.Yaqoob AA, Ahmad H, Parveen T, Ahmad A, Oves M, Ismail IM, Qari HA, Umar K, Mohamad Ibrahim MN. Recent advances in metal decorated nanomaterials and their various biological applications: a review. Front Chem. 2020; 8: 341.
17.Piacenza E, Presentato A, Heyne B, Turner RJ. Tunable photoluminescence properties of selenium nanoparticles: biogenic versus chemogenic synthesis. Nanophotonics. 2020; 9(11): 3615.
18.Busi S, Rajkumari J. Chapter 15 - Microbially synthesized nanoparticles as next generation antimicrobials: scope and applications. In: Grumezescu AM, editor. Nanoparticles in Pharmacotherapy: William Andrew Publishing; 2019. p. 485-524.
19.Ovais M, Khalil AT, Ayaz M, Ahmad I, Nethi SK, Mukherjee S. Biosynthesis of metal nanoparticles via microbial enzymes: a mechanistic approach. Int J Mol Sci. 2018; 19(12): 4100.
20.Priyanka B. Biosynthesis of silver nanoparticles from Aspergillus flavus. J Pharm Sci Res. 2020; 12(4): 583-586.
21.Fan D, Li L, Li Z, Zhang Y, Ma X, Wu L, Zhang H, Guo F. Biosynthesis of selenium nanoparticles and their protective, antioxidative effects in streptozotocin induced diabetic rats. Sci Technol Adv Mater. 2020; 21(1): 505-514.
22.Bai K, Hong B, Huang W, He J. Selenium-nanoparticles-loaded chitosan/chitooligosaccharide microparticles and their antioxidant potential: a chemical and in vivo investigation. Pharmaceutics. 2020; 12(1): 43.
23.Sonkusre P. Specificity of biogenic selenium nanoparticles for prostate cancer therapy with reduced risk of toxicity: an in vitro and in vivo study. Front Oncol. 2020; 9(1541): 1541.
24.Guo L, Xiao J, Liu H, Liu H. Selenium nanoparticles alleviate hyperlipidemia and vascular injury in ApoE-deficient mice by regulating cholesterol metabolism and reducing oxidative stress. Metallomics. 2020; 12(2): 204-217.
25.Ghomi ARG, Mohammadi-Khanaposhti M, Vahidi H, Kobarfard F, Reza MAS, Barabadi H. Fungus-mediated extracellular biosynthesis and characterization of zirconium nanoparticles using standard penicillium species and their preliminary bactericidal potential: a novel biological approach to nanoparticle synthesis. Iran J Pharm Res. 2019; 18(4): 2101.
26.Barabadi H, Kobarfard F, Vahidi H. Biosynthesis and characterization of biogenic tellurium nanoparticles by using Penicillium chrysogenum PTCC 5031: a novel approach in gold biotechnology. Iran J Pharm Res. 2018; 17(Suppl2): 87.
27.Vahdati M, Tohidi Moghadam T. Synthesis and characterization of selenium nanoparticles-lysozyme nanohybrid system with synergistic antibacterial properties. Sci Rep. 2020; 10(1): 510.
28.Faramarzi S, Anzabi Y, Jafarizadeh-Malmiri H. Nanobiotechnology approach in intracellular selenium nanoparticle synthesis using Saccharomyces cerevisiae—fabrication and characterization. Arch Microbiol. 2020; 202(5): 1203-1209.
29.Liang T, Qiu X, Ye X, Liu Y, Li Z, Tian B, Yan D. Biosynthesis of selenium nanoparticles and their effect on changes in urinary nanocrystallites in calcium oxalate stone formation. 3 Biotech. 2019; 10(1): 23.
30.Barabadi H. Nanobiotechnology: a promising scope of gold biotechnology. Cell Mol Biol. 2017; 63(12): 3-4.
31.Saravanan M, Ramachandran B, Barabadi H. The prevalence and drug resistance pattern of extended spectrum β–lactamases (ESBLs) producing Enterobacteriaceae in Africa. Microb Pathog. 2018; 114: 180-192.
32.Busi S, Rajkumari J. Chapter 15 - Microbially synthesized nanoparticles as next generation antimicrobials: scope and applications. In: Grumezescu AM, editor. Nanoparticles in Pharmacotherapy: William Andrew Publishing; 2019. p. 485-524.
33.Gunti L, Dass RS, Kalagatur NK. Phytofabrication of selenium nanoparticles from Emblica officinalis fruit extract and exploring its biopotential applications: antioxidant, antimicrobial, and biocompatibility. Front Microbiol. 2019; 10: 931.
34.Tran PA, O’Brien-Simpson N, Reynolds EC, Pantarat N, Biswas DP, O’Connor AJ. Low cytotoxic trace element selenium nanoparticles and their differential antimicrobial properties against S. aureus and E. coli. Nanotechnology. 2016; 27(4): 045101.
35.Kokila K, Elavarasan N, Sujatha V. Diospyros montana leaf extract-mediated synthesis of selenium nanoparticles and their biological applications. New J Chem. 2017; 41(15): 7481-7490.
36.Guisbiers G, Wang Q, Khachatryan E, Mimun LC, Mendoza-Cruz R, Larese-Casanova P, Webster TJ, Nash KL. Inhibition of E. coli and S. aureus with selenium nanoparticles synthesized by pulsed laser ablation in deionized water. Int J Nanomed. 2016; 11: 3731.
37.Tran PA, O’Brien-Simpson N, Palmer JA, Bock N, Reynolds EC, Webster TJ, Deva A, Morrison WA, O’connor AJ. Selenium nanoparticles as anti-infective implant coatings for trauma orthopedics against methicillin-resistant Staphylococcus aureus and epidermidis: in vitro and in vivo assessment. Int J Nanomed. 2019; 14: 4613.
38.Zonaro E, Lampis S, Turner RJ, Qazi SJS, Vallini G. Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms. Front Microbiol. 2015; 6: 584.