Sublingual immunotherapy by Nanogold in mice model of asthma

Document Type : Research Paper

Authors

1 Department of Biology, Faculty of Science, Islamic Azad University, Mashhad, Iran

2 Department of Internal Medicine, Mashhad Medical Sciences Branch, Islamic Azad University, Mashhad, Iran

3 Innovative Medical Research Center and Department of Immunology, Mashhad Branch Islamic Azad University, Mashhad, Iran

Abstract

Objective(s): It has been shown that Nanogold particles have anti-inflammatory effects in different Rheumatologic, neurologic and gastrointestinal disease. They inhibit the synthesis of pro-inflammatory cytokines and also infiltration of inflammatory cells. Sublingual immunotherapy is a well-known effective, safe and clinically effective method way of immune response regulation which results in long-lasting symptoms reduction.
This research was designed to find the immunological effects of sublingual immunotherapy using Nanogold in mice model of asthma.
Materials and Methods: Twenty BALB/c mice were divided into four groups including one group of non-sensitized mice and three groups of asthmatic mice which were treated sublingually with PBS, Nanogold and Beclomethasone. IL-4 and IFN-γ levels were measured in serum and spleen cells supernatant using ELISA. BAL fluid inflammatory cells differential counting and lungs histological analysis were also done.
Results: The results revealed that there was significant increase in level of IFN-γ and decrease in level of IL-4 in serum and spleen cells supernatant of Nanogold treated group (p <0.05). These findings indicates the shift of Th2/Th1 balance towards Th1 cells which is protective against asthma. In addition, histological and BAL fluid analysis demonstrated the reduction of cells and eosinophilic infiltration.
Conclusion: Based on our results, sublingual immunotherapy by Nanogold has significant anti-inflammatory roll in asthmatic mice. Thus, Nanogold is a potentially valuable agent for controlling the underlying inflammation in asthma. However, further investigations is recommended to find more details about its effects.

Keywords

References

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Nanomedicine Journal
Volume 8, Issue 2
April 2021
Pages 124-131

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