Green synthesis of glucose-coated gold nanoparticles for improving radiosensitivity in human U87 glioblastoma cell line

Document Type : Research Paper

Authors

1 Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

2 Department of Biochemistry and Nutrition, School of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran

3 Medical Physics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

4 Department of Medical Biotechnology and Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

Abstract

Objective(s): Surgery and radiation therapy are the most important known treatments for glioblastoma, which is known as the most malignant tumor of the central nervous system. Numerous studies have proven the effect of different gold nanoparticles in improving radiation sensitivity. But there is still a need for nanoparticles with suitable size and higher sensitivity. Hence, the present study aimed to prepare optimized glucose-coated gold nanoparticles (Glu-GNPs) for improving radiosensitivity against U87 glioblastoma cells.
Materials and Methods: Firstly, Glu-GNPs were synthesized and then their physiochemical characterizations were assessed using dynamic light scattering (DLS). The cytotoxicity of Glu-GNPs was evaluated by MTT assay in U87 and NIH-3T3 cell lines. Additionally, the colony formation assay, which is known as the gold standard test, was used to evaluate the radiosensitivity effect of Glu-GNPs on U87 cells.
Results: The characterization results showed that Glu-GNPs had a size of 50.3 nm and negative zeta potential of -13.8 mV. Cytotoxicity results revealed that treatment with Glu-GNPs significantly inhibited the proliferation of U87 cells. We found that Glu-GNPs at a concentration of 10 μg/ml were not-toxic for U87 cells. Moreover, the colony formation assay results showed that Glu-GNPs significantly increased the effect of radiation and caused U87 cancer cell death at a non-toxic concentration of 10 μg/ml.
Conclusion: Taken together, the Glu-GNPs, with a size of 50.3 nm, increased radiosensitivity and caused cell death at a concentration of 10 µg/ml in U87 glioblastoma cells and deserve further in vitro and in vivo investigations.

Keywords

References

1.  Alexander BM, Cloughesy TF. Adult glioblastoma. Am J Clin Oncol. 2017;35(21):2402-2409.
2.  Ohgaki H, Kleihues P. Genetic pathways to primary and secondary glioblastoma. Am J Clin Pathol. 2007;170(5):1445-1453.
3.  Davis ME. Glioblastoma: overview of disease and treatment. Clin J Oncol Nurs. 2016;20(5):S2.
4.  Weller M, Cloughesy T, Perry JR, Wick W. Standards of care for treatment of recurrent glioblastoma—are we there yet? Neuro-Oncol. 2013;15(1):4-27.
5.  Kobayashi K, Usami N, Porcel E, Lacombe S, Le Sech C. Enhancement of radiation effect by heavy elements. Mutat Res Rev Mutat Res. 2010;704(1-3):123-131.
6.  Ward JF. DNA damage produced by ionizing radiation in mammalian cells: identities, mechanisms of formation, and reparability. Prog Nucleic Acid Res Mol Biol. 1988;35:95-125.
7.  Su X-Y, Liu P-D, Wu H, Gu N. Enhancement of radiosensitization by metal-based nanoparticles in cancer radiation therapy. Cancer Biol Med. 2014;11(2):86.
8.  Riley P. Free radicals in biology: oxidative stress and the effects of ionizing radiation. Int J Radiat Biol. 1994;65(1):27-33.
9.  Matsudaira H, Ueno AM, Furuno I. Iodine contrast medium sensitizes cultured mammalian cells to X rays but not to γ rays. Radiat Res. 1980;84(1):144-148.
10.  Choi J, Kim G, Cho SB, Im H-J. Radiosensitizing high-Z metal nanoparticles for enhanced radiotherapy of glioblastoma multiforme. J Nanobiotechnology. 2020;18(1):1-23.
11.  Giljohann DA, Seferos DS, Daniel WL, Massich MD, Patel PC, Mirkin CA. Gold nanoparticles for biology and medicine. Spherical Nucleic Acids. 2020:55-90.
12.  Vakili-Ghartavol R, Mehrabian A, Mirzavi F, Rezayat SM, Mashreghi M, Farhoudi L, et al. Docetaxel in combination with metformin enhances antitumour efficacy in metastatic breast carcinoma models: a promising cancer targeting based on PEGylated liposomes. Pharm Pharmacol. 2022;74(9):1307-1319.
13.  Boisselier E, Astruc D. Gold nanoparticles in nanomedicine: preparations, imaging, diagnostics, therapies and toxicity. Chem Soc Rev. 2009;38(6):1759-1782.
14.  Babaei M, Ganjalikhani M. A systematic review of gold nanoparticles as novel cancer therapeutics. Nanomed J. 2014;1(4):211-219.
15.  Sperling RA, Gil PR, Zhang F, Zanella M, Parak WJ. Biological applications of gold nanoparticles. Chem Soc Rev. 2008;37(9):1896-1908.
16.  Chithrani BD, Ghazani AA, Chan WC. Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells. Nano Lett. 2006;6(4):662-668.
17.  Cho EC, Au L, Zhang Q, Xia Y. The effects of size, shape, and surface functional group of gold nanostructures on their adsorption and internalization by cells. Small. 2010;6(4):517-522.
18.  Trono JD, Mizuno K, Yusa N, Matsukawa T, Yokoyama K, Uesaka M. Size, concentration and incubation time dependence of gold nanoparticle uptake into pancreas cancer cells and its future application to X-ray drug delivery system. J Radiat Res. 2011;52(1):103-109.
19.  Kong T, Zeng J, Wang X, Yang X, Yang J, McQuarrie S, et al. Enhancement of radiation cytotoxicity in breast‐cancer cells by localized attachment of gold nanoparticles. small. 2008;4(9):1537-1543.
20.  Wang C, Jiang Y, Li X, Hu L. Thioglucose-bound gold nanoparticles increase the radiosensitivity of a triple-negative breast cancer cell line (MDA-MB-231). Breast Cancer. 2015;22(4):413-420.
21.  Wang C, Li X, Wang Y, Liu Z, Fu L, Hu L. Enhancement of radiation effect and increase of apoptosis in lung cancer cells by thio-glucose-bound gold nanoparticles at megavoltage radiation energies. J Nanoparticle Res. 2013;15(5):1-12.
22.  Zhang X, Xing JZ, Chen J, Ko L, Amanie J, Gulavita S, et al. Enhanced radiation sensitivity in prostate cancer by gold-nanoparticles. Clin Invest Med. 2008:E160-E167.
23.  Liu J, Qin G, Raveendran P, Ikushima Y. Facile “green” synthesis, characterization, and catalytic function of β‐D‐glucose‐stabilized Au nanocrystals. Eur J Chem. 2006;12(8):2131-2138.
24.  Maharramov A, Ramazanov M, Shabanov A, Eyvazova Q, Agamaliyev Z, Hajiyeva F, et al. Preparation of 2-deoxy-D-glucose coated spio nanoparticles and characterization of their physical, chemical, and biological properties. Dig. J. Nanomater. Biostructures. 2014;9(4):1461-1469.
25.  Suvarna S, Das U, Kc S, Mishra S, Sudarshan M, Saha KD, et al. Synthesis of a novel glucose capped gold nanoparticle as a better theranostic candidate. PLoS One. 2017;12(6):e0178202.
26.  Ibrahim M, Alaam M, El-Haes H, Jalbout AF, Leon Ad. Analysis of the structure and vibrational spectra of glucose and fructose. Eclética Quim J. 2006;31:15-21.
27.  Babaei M, Ganjalikhani M. The potential effectiveness of nanoparticles as radio sensitizers for radiotherapy. BioImpacts: BI. 2014;4(1):15.
28.  Santhosh PB, Genova J, Chamati H. Green Synthesis of Gold Nanoparticles: An Eco-Friendly Approach. Chemistry. 2022;4(2):345-369.
29.  Mirzavi F, Barati M, Vakili-Ghartavol R, Roshan MK, Mashreghi M, Soukhtanloo M, et al. Pegylated liposomal encapsulation improves the antitumor efficacy of combretastatin A4 in murine 4T1 triple-negative breast cancer model. Int J Pharm. 2022;613:121396.
30.  Bhattacharyya S, Kudgus RA, Bhattacharya R, Mukherjee P. Inorganic nanoparticles in cancer therapy. Pharm Res. 2011;28(2):237-259.
31.  Geng F, Xing JZ, Chen J, Yang R, Hao Y, Song K, et al. Pegylated glucose gold nanoparticles for improved in-vivo bio-distribution and enhanced radiotherapy on cervical cancer. J Biomed Nanotechnol. 2014;10(7):1205-1216.
32.  Roa W, Zhang X, Guo L, Shaw A, Hu X, Xiong Y, et al. Gold nanoparticle sensitize radiotherapy of prostate cancer cells by regulation of the cell cycle. Nanotechnology. 2009;20(37):375101.
Nanomedicine Journal
Volume 9, Issue 4
October 2022
Pages 328-333

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