Investigation and comparison of laser and ultrasound effects on the temperature increasing of the solutions containing graphene oxide nanoparticles for thermal treatment of osteosarcoma cancer cells

Document Type : Research Paper

Authors

1 Department of Biomedical Engineering, Meybod University, PO Box 89616-99557, Meybod, Iran

2 Department of Biology, Taft Payame Noor University, Yazd, Iran

3 Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

4 Department of Computer Engineering, Meybod University, Meybod, Iran

5 Department of Nursing, Shirvan Faculty of Nursing, North Khorasan University of Medical Sciences, Bojnūrd, Iran

6 Department of Advanced Technologies, School of Medicine, North Khorasan University of Medical Sciences, Bojnūrd, Iran

7 Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnūrd, Iran

Abstract

Objective(s): The waves of ultrasound and laser in the presence of nanoparticles are introduced as desirable candidates for the thermal treatment of cancer due to having fewer side effects, more speed, and superior treatment efficiency. Here, 2D Graphene oxide nanoparticle is used as a thermal nano-convertor for increasing the yield of thermal cancer therapy.
Materials and Methods: The temperature of GO (in 0.2 and 0.4 mg/ml concentrations) and deionized water regarding heater, bath sonicate, probe sonicate (at a power range of 2-3.5 W), and laser properties at 808 nm with continuous wave (at a power of 0-2 W) in 10 min are investigated. Based on the experimental results, the effect of laser and ultrasound radiation on the temperature is simulated using a data mining approach.
Results: Experimental and simulation results show that GO nanoparticle in this form is unsuitable for converting ultrasound waves into heat. But it is a strong absorber for electromagnetic waves at 808 nm and can raise the temperature to 85 °C. The results indicate that the laser + GO enhances the mortality percentage and treatment yield of MG63 cancerous cells by up to 85%. Also, GO uptake is analyzed by fluorescent microscopic images.
Conclusion: This analysis confirmed that GO is important when laser radiation is used but not when Ultrasound is employed. Also, GO is an excellent photothermal nanoparticle for localized thermal therapy of osteosarcoma cancer cells by laser at 808 nm with low side effects.

Keywords

References

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Nanomedicine Journal
Volume 10, Issue 4
October 2023
Pages 313-322

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