1. Mason C, Manzotti E. Regenerative medicine cell therapies: numbers of units manufactured and patients treated between 1988 and 2010. Regen Med. 2010;5(3):307-313.
2. Yu Y, Wang Q, Wang C, Shang L. Living materials for regenerative medicine. Engin Regen. 2021;2:96-104.
3. Sampogna G, Guraya SY, Forgione A. Regenerative medicine: Historical roots and potential strategies in modern medicine. J Microsc Ultrastruct. 2015;3(3):101-107.
4. Fritsma GA. Platelet structure and function. Clinical laboratory science. 2015;28(2):125-131.
5. van der Meijden PE, Heemskerk JW. Platelet biology and functions: new concepts and clinical perspectives. Nat Rev Cardiol. 2019;16(3):166-179.
6. Emer J. Platelet-rich plasma (PRP): current applications in dermatology. Skin Therapy Lett. 2019;24(5):1-6.
7. Lubkowska A, Dolegowska B, Banfi G. Growth factor content in PRP and their applicability in medicine. J Biol Regul Homeost Agents. 2012;26(2 Suppl 1):3S-22S.
8. Oneto P, Etulain J. PRP in wound healing applications. Platelets. 2021;32(2):189-199.
9. Davis ME. Exosomes: what do we love so much about them? Circ Res. 2016;119(12):1280-1282.
10. Sharma S, Rasool HI, Palanisamy V, Mathisen C, Schmidt M, Wong DT, et al. Structural-mechanical characterization of nanoparticle exosomes in human saliva, using correlative AFM, FESEM, and force spectroscopy. ACS Nano. 2010;4(4):1921-1926.
11. Ha D, Yang N, Nadithe V. Exosomes as therapeutic drug carriers and delivery vehicles across biological membranes: current perspectives and future challenges. Acta Pharm Sin B. 2016;6(4):287-296.
12. Suchorska WM, Lach MS. The role of exosomes in tumor progression and metastasis. Oncol Rep. 2016;35(3):1237-1244.
13. Qin J, Xu Q. Functions and application of exosomes. Acta Pol Pharm. 2014;71(4):537-543.
14. Van Niel G, d’Angelo G, Raposo G. Shedding light on the cell biology of extracellular vesicles. Nat Rev Mol Cell Biol. 2018;19(4):213-228.
15. Haney MJ, Klyachko NL, Zhao Y, Gupta R, Plotnikova EG, He Z, et al. Exosomes as drug delivery vehicles for Parkinson’s disease therapy. J Control Release. 2015;207:18-30.
16. Liang Y, Duan L, Lu J, Xia J. Engineering exosomes for targeted drug delivery. Theranostics. 2021;11(7):3183.
17. Aatonen M, Grönholm M, Siljander PR. Platelet-derived microvesicles: Multitalented participants in intercellular communication. Semin Thromb Hemost. 2012;38(1):102-113.
18. Wolf P. The nature and significance of platelet products in human plasma. Br J Haematol. 1967;13(3):269-288.
19. Sinauridze EI, Kireev DA, Popenko NY, Pichugin AV, Panteleev MA, Krymskaya OV, et al. Platelet microparticle membranes have 50-to 100-fold higher specific procoagulant activity than activated platelets. Thromb Haemost. 2007;97(03):425-434.
20. Wu J, Piao Y, Liu Q, Yang X. Platelet‐rich plasma‐derived extracellular vesicles: A superior alternative in regenerative medicine? Cell Prolif. 2021;54(12):e13123.
21. Torreggiani E, Perut F, Roncuzzi L, Zini N, Baglìo S, Baldini N. Exosomes: novel effectors of human platelet lysate activity. Eur Cell Mater. 2014;28:137-151.
22. Xu Y, Lin Z, He L, Qu Y, Ouyang L, Han Y, et al. Platelet-rich plasma-derived exosomal USP15 promotes cutaneous wound healing via deubiquitinating EIF4A1. Oxid Med Cell Longev. 2021;2021.
23. Spakova T, Janockova J, Rosocha J. Characterization and therapeutic use of extracellular vesicles derived from platelets. Int J Mol Sci. 2021;22(18):9701.
24. Théry C, Witwer KW, Aikawa E, Alcaraz MJ, Anderson JD, Andriantsitohaina R, et al. Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. J Extracell Vesicles. 2018;7(1):1535750.
25. Antwi‐Baffour S, Adjei J, Aryeh C, Kyeremeh R, Kyei F, Seidu MA. Understanding the biosynthesis of platelets‐derived extracellular vesicles. Immun Inflamm Dis. 2015;3(3):133-140.
26. Coumans FA, Brisson AR, Buzas EI, Dignat-George F, Drees EE, El-Andaloussi S, et al. Methodological guidelines to study extracellular vesicles. Circ Res. 2017;120(10):1632-1648.
27. Wisgrill L, Lamm C, Hartmann J, Preißing F, Dragosits K, Bee A, et al. Peripheral blood microvesicles secretion is influenced by storage time, temperature, and anticoagulants. Cytometry A. 2016;89(7):663-672.
28. Tao S-C, Yuan T, Rui B-Y, Zhu Z-Z, Guo S-C, Zhang C-Q. Exosomes derived from human platelet-rich plasma prevent apoptosis induced by glucocorticoid-associated endoplasmic reticulum stress in rat osteonecrosis of the femoral head via the Akt/Bad/Bcl-2 signal pathway. Theranostics. 2017;7(3):733-750.
29. Sidhom K, Obi PO, Saleem A. A review of exosomal isolation methods: is size exclusion chromatography the best option?. Int J Mol Sci. 2020;21(18):6466.
30. Livshits MA, Khomyakova E, Evtushenko EG, Lazarev VN, Kulemin NA, Semina SE, et al. Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol. Sci Rep. 2015;5(1):17319.
31. Konoshenko MY, Lekchnov EA, Vlassov AV, Laktionov PP. Isolation of extracellular vesicles: general methodologies and latest trends. Biomed Res Int. 2018;2018.
32. Koliha N, Wiencek Y, Heider U, Jüngst C, Kladt N, Krauthäuser S, et al. A novel multiplex bead-based platform highlights the diversity of extracellular vesicles. J Extracell Vesicles. 2016;5(1):29975.
33. Rui S, Yuan Y, Du C, Song P, Chen Y, Wang H, et al. Comparison and investigation of exosomes derived from platelet-rich plasma activated by different agonists. Cell Transplant. 2021;30:09636897211017833.
34. Saumell-Esnaola M, Delgado D, García del Caño G, Beitia M, Sallés J, González-Burguera I, et al. Isolation of platelet-derived exosomes from human platelet-rich plasma: Biochemical and morphological characterization. Int J Mol Sci. 2022;23(5):2861.
35. Yakubovich E, Polischouk A, Evtushenko V. Principles and problems of exosome isolation from biological fluids. Biochem (Mosc) Suppl Ser A Membr Cell Biol. 2022;16(2):115-126.
36. Pinedo H, Verheul H, D’amato R, Folkman J. Involvement of platelets in tumour angiogenesis? . Lancet. 1998;352(9142):1775-1777.
37. Brill A, Elinav H, Varon D. Differential role of platelet granular mediators in angiogenesis. Cardiovasc Res. 2004;63(2):226-235.
38. Brill A, Dashevsky O, Rivo J, Gozal Y, Varon D. Platelet-derived microparticles induce angiogenesis and stimulate post-ischemic revascularization. Cardiovasc Res. 2005;67(1):30-38.
39. Bordin A, Chirivì M, Pagano F, Milan M, Iuliano M, Scaccia E, et al. Human platelet lysate‐derived extracellular vesicles enhance angiogenesis through miR‐126. Cell Prolif. 2022;55(11):e13312.
40. Li Q, Song Y, Wang Q, Chen J, Gao J, Tan H, et al. Engineering extracellular vesicles with platelet membranes fusion enhanced targeted therapeutic angiogenesis in a mouse model of myocardial ischemia reperfusion. Theranostics. 2021;11(8):3916-3931.
41. Paus R, Cotsarelis G. The biology of hair follicles. N Engl J Med. 1999;341(7):491-497.
42. Cash T. The psychosocial consequences of androgenetic alopecia: a review of the research literature. Br J Dermatol. 1999;141(3):398-405.
43. Wolff H, Fischer TW, Blume-Peytavi U. The diagnosis and treatment of hair and scalp diseases. Dtsch Arztebl Int. 2016;113(21):377-386.
44. Randolph M, Tosti A. Oral minoxidil treatment for hair loss: a review of efficacy and safety. J Am Acad Dermatol. 2021;84(3):737-746.
45. Zgonc Škulj A, Poljšak N, Kočevar Glavač N, Kreft S. Herbal preparations for the treatment of hair loss. Arch Dermatol Res. 2020;312:395-406.
46. Badran KW, Sand JP. Platelet-rich plasma for hair loss: review of methods and results. Facial Plast Surg Clin North Am. 2018;26(4):469-485.
47. Ekelem C, Pham C, Mesinkovska NA. A systematic review of the outcome of hair transplantation in primary scarring alopecia. Skin Appendage Disord. 2019;5(2):65-71.
48. Kalabusheva E, Terskikh V, Vorotelyak E. Hair germ model in vitro via human postnatal keratinocyte-dermal papilla interactions: impact of hyaluronic acid. Stem Cells Int. 2017;2017.
49. Bukvić Mokoš Z, Lazić Mosler E. Advances in a rapidly emerging field of hair follicle stem cell research. Coll Antropol. 2014;38(1):373-378.
50. Yang C-C, Cotsarelis G. Review of hair follicle dermal cells. J Dermatol Sci. 2010;57(1):2-11.
51. Zhu N, Huang K, Liu Y, Zhang H, Lin E, Zeng Y, et al. miR-195-5p regulates hair follicle inductivity of dermal papilla cells by suppressing Wnt/β-catenin activation. Biomed Res Int. 2018;2018.
52. Phinney DG, Pittenger MF. Concise review: MSC-derived exosomes for cell-free therapy. Stem cells. 2017;35(4):851-858.
53. Nilforoushzadeh MA, Aghdami N, Taghiabadi E. Human hair outer root sheath cells and platelet-lysis exosomes promote hair inductivity of dermal papilla cell. Tissue Eng Regen Med. 2020;17:525-536.
54. Nilforoushzadeh MA, Aghdami N, Taghiabadi E. Effects of Adipose-Derived Stem Cells and Platelet-Rich Plasma Exosomes on The Inductivity of Hair Dermal Papilla Cells. Cell J. 2021;23(5):576-583.
55. Kyaw B, Jaerbrink K, Martinengo L, Car J, Harding K, Schmidtchen A. Need for improved definition of “chronic wounds” in clinical studies. Acta Derm Venereol. 2017;98(1):157-158.
56. Sen CK. Human Wounds and Its Burden: An Updated Compendium of Estimates. Adv Wound Care (New Rochelle). 2019;8(2):39-48.
57. Morton LM, Phillips TJ. Wound healing and treating wounds: Differential diagnosis and evaluation of chronic wounds. J Am Acad Dermatol. 2016;74(4):589-605.
58. PC S. Gingival Wound Healing. J Dent Res. 2014;94(3):395-402.
59. Etulain J. Platelets in wound healing and regenerative medicine. Platelets. 2018;29(6):556-568.
60. Wu P, Tong Z, Luo L, Zhao Y, Chen F, Li Y, et al. Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair. Bioact Mater. 2021;6(10):3515-3527.
61. Shi A, Li J, Qiu X, et al. TGF-β loaded exosome enhances ischemic wound healing in vitro and in vivo. Theranostics. 2021;11(13):6616-6631. Published 2021.
62. Singh G, Borah D, Khanna G, Jain S. Efficacy of local autologous platelet-rich plasma in the treatment of pressure ulcer in spinal cord injury patients. Cureus. 2021;13(10).
63. Habib TN, Fayed AM, Deghady AA, Maklad OM, Ahmed IE. Autologous platelet-rich plasma in the treatment of third-degree pressure ulcers; pilot randomized-controlled trial. Research and Opinion in Anesthesia & Intensive Care. 2023;10(3):236-241.
64. Swarnakar R, Rahman H, Venkataraman S. “Platelet-Rich Fibrin Membrane-as a novel biomaterial for pressure injury healing in a person with spinal cord injury: A case report”. Spinal Cord Ser Cases. 2022;8(1):75. Published 2022.
65. Guo SC, Tao SC, Yin WJ, Qi X, Yuan T, Zhang CQ. Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model. Theranostics. 2017;7(1):81-96.
66. Chen T, Song P, He M, et al. Sphingosine-1-phosphate derived from PRP-Exos promotes angiogenesis in diabetic wound healing via the S1PR1/AKT/FN1 signalling pathway. Burns Trauma. 2023;11:tkad003.
67. Antich-Rosselló M, Munar-Bestard M, Forteza-Genestra MA, Calvo J, Gayà A, Monjo M, et al. Platelet Derived Extracellular Vesicles Promote in vitro Gingival Wound Healing. 2021.
68. Zhang W, Dong X, Wang T, Kong Y. Exosomes derived from platelet-rich plasma mediate hyperglycemia-induced retinal endothelial injury via targeting the TLR4 signaling pathway. Exp Eye Res. 2019;189:107813.
69. Zhang W, Jiang H, Kong Y. Exosomes derived from platelet-rich plasma activate YAP and promote the fibrogenic activity of Müller cells via the PI3K/Akt pathway. Exp Eye Res. 2020;193:107973.
70. Widyaningrum R, Wu Y-W, Delila L, Lee D-Y, Wang T-J, Burnouf T. In vitro evaluation of platelet extracellular vesicles (PEVs) for corneal endothelial regeneration. Platelets. 2022;33(8):1237-1250.
71. Tammam BM, Habotta OA, El-Khadragy M, Moneim AEA, Abdalla MS. Therapeutic role of mesenchymal stem cells and platelet-rich plasma on skin burn healing and rejuvenation: A focus on scar regulation, oxido-inflammatory stress and apoptotic mechanisms. Heliyon. 2023;9(9).
72. Imam R, Amer M. Potential therapeutic role of microvesicles derived from mesenchymal stem cells and platelet-rich plasma in murine burn wound healing: scar regulation and antioxidant mechanism. Folia Morphol (Warsz). 2023;82(3):656-667.
73. Dayan S, Gandhi N, Wilson J, Kola E, Jankov LE, Copeland K, et al. Safety and efficacy of human platelet extract in skin recovery after fractional CO2 laser resurfacing of the face: A randomized, controlled, evaluator‐blinded pilot study. J Cosmet Dermatol. 2023;22(9):2464-2470.
74. Proffer SL, Paradise CR, DeGrazia E, Halaas Y, Durairaj KK, Somenek M, et al. Efficacy and tolerability of topical platelet exosomes for skin rejuvenation: six-week results. Aesthet Surg J. 2022;42(10):1185-1193.
75. Rosmarwati E, Ellistasari EY, Kusumawardani A, Julianto I, Widhiati S, Setyawan NA, et al. Human platelet lysate-derived exosomes are superior to the lysate at increasing collagen deposition in a rat model of intrinsic aging. Journal of Applied Pharmaceutical Science. 2023:211-216.
76. Fang J, Wang X, Jiang W, Zhu Y, Hu Y, Zhao Y, et al. Platelet-rich plasma therapy in the treatment of diseases associated with orthopedic injuries. Tissue Eng Part B Rev. 2020;26(6):571-585.
77. Iyer SR, Scheiber AL, Yarowsky P, Henn III RF, Otsuru S, Lovering RM. Exosomes isolated from platelet-rich plasma and mesenchymal stem cells promote recovery of function after muscle injury. Am J Sports Med . 2020;48(9):2277-2286.
78. Graça AL, Domingues RM, Calejo I, Gómez-Florit M, Gomes ME. Therapeutic effects of platelet-derived extracellular vesicles in a bioengineered tendon disease model. Int J Mol Sci. 2022;23(6):2948.
79. Zhang Y, Wang X, Chen J, Qian D, Gao P, Qin T, et al. Exosomes derived from platelet-rich plasma administration in site mediate cartilage protection in subtalar osteoarthritis. J Nanobiotechnology. 2022;20(1):1-22.
80. Zhou Y, Wang T, Hamilton JL, Chen D. Wnt/β-catenin signaling in osteoarthritis and in other forms of arthritis. Curr Rheumatol Rep. 2017;19:1-8.
81. Liu X, Wang L, Ma C, Wang G, Zhang Y, Sun S. Exosomes derived from platelet-rich plasma present a novel potential in alleviating knee osteoarthritis by promoting proliferation and inhibiting apoptosis of chondrocyte via Wnt/β-catenin signaling pathway. J Orthop Surg Res. 2019;14(1):1-16.
82. Wilczewska AZ, Niemirowicz K, Markiewicz KH, Car H. Nanoparticles as drug delivery systems. Pharmacol Rep. 2012;64(5):1020-1037.
83. Li C, Wang J, Wang Y, Gao H, Wei G, Huang Y, et al. Recent progress in drug delivery. Acta Pharm Sin B. 2019;9(6):1145-1162.
84. Heidarzadeh M, Gürsoy-Özdemir Y, Kaya M, Eslami Abriz A, Zarebkohan A, Rahbarghazi R, et al. Exosomal delivery of therapeutic modulators through the blood–brain barrier; promise and pitfalls . Cell Biosci. 2021;11:1-28.
85. Meliciano A, Salvador D, Mendonça P, Louro AF, Serra M. Clinically Expired Platelet Concentrates as a Source of Extracellular Vesicles for Targeted Anti-Cancer Drug Delivery. Pharmaceutics. 2023;15(3):953.
86. Uslu D, Abas BI, Demirbolat GM, Cevik O. Effect of platelet exosomes loaded with doxorubicin as a targeted therapy on triple-negative breast cancer cells. Mol Divers. 2024;28(2):449-460.
87. Soleymani S, Yari F, Bolhassani A, Bakhshandeh H. Platelet microparticles: An effective delivery system for anti-viral drugs. J Drug Deliv Sci Technol. 2019;51:290-296.
88. Rezaei R, Baghaei K, Amani D, Piccin A, Hashemi SM, Aghdaei HA, et al. Exosome-mediated delivery of functionally active miRNA-375-3p mimic regulate epithelial mesenchymal transition (EMT) of colon cancer cells. Life Sci. 2021;269:119035.
89. Shams SF, Deyhim M. Platelet-Derived Exosomes as the Nano-Carrier for Mir-150 to Modulate Gene Expression and Cell Cycle in the M07-E Cell Line.
90. Shams SF, Mohammadipour M, Deyhim M. Preparation and characterization of platelet-derived exosomes, as a nanostructure for bio-compound delivery. Nanomed J. 2023;10(2).
91. Yao Y, Sun W, Sun Q, Jing B, Liu S, Liu X, et al. Platelet-derived exosomal MicroRNA-25-3p inhibits coronary vascular endothelial cell inflammation through Adam10 via the NF-κB signaling pathway in ApoE−/− mice. Front Immunol. 2019;10:2205.
92. Tan M, Yan H-B, Li J-N, Li W-K, Fu Y-Y, Chen W, et al. Thrombin stimulated platelet-derived exosomes inhibit platelet-derived growth factor receptor-beta expression in vascular smooth muscle cells. Cell Physiol Biochem. 2016;38(6):2348-2365.
93. Bai X, Zhang H, Li Z, Chen O, He H, Jia X, et al. Platelet-derived extracellular vesicles encapsulate microRNA-34c-5p to ameliorate inflammatory response of coronary artery endothelial cells via PODXL-mediated P38 MAPK signaling pathway. Nutr Metab Cardiovasc Dis. 2022;32(10):2424-2438.
94. Arul S, Peterson T, Behfar A, Kadirvel R, Kallmes D. O-050 Human platelet-derived exosomes as bioactive material for aneurysm treatment with platinum coils: in vitro proof of concept study. British Med J. 2023.
95. Hayon Y, Dashevsky O, Shai E, Brill A, Varon D, R Leker R. Platelet microparticles induce angiogenesis and neurogenesis after cerebral ischemia. Curr Neurovasc Res. 2012;9(3):185-192.
96. Wang Z, Zheng Y, Cai H, Yang C, Li S, Lv H, et al. Aβ1-42-containing platelet-derived extracellular vesicle is associated with cognitive decline in Parkinson’s disease. Front Aging Neurosci. 2023;15:1170663.
97. Bortot B, Mangogna A, Peacock B, Lees R, Valle F, Brucale M, et al. Platelet Activation in Ovarian Cancer Ascites: Assessment of GPIIb/IIIa and PF4 in Small Extracellular Vesicles by Nano-Flow Cytometry Analysis. Cancers (Basel). 2022;14(17):4100.
98. Vismara M, Zarà M, Negri S, Canino J, Canobbio I, Barbieri SS, et al. Platelet-derived extracellular vesicles regulate cell cycle progression and cell migration in breast cancer cells. Biochim Biophys Acta Mol Cell Res. 2021;1868(1):118886.
99. Jiao Y, Li W, Wang W, Tong X, Xia R, Fan J, et al. Platelet-derived exosomes promote neutrophil extracellular trap formation during septic shock. Crit Care. 2020;24(1):1-18.
100. Lee BK, Kim MH, Lee SY, Son SJ, Hong CH, Jung Y-S. Downregulated Platelet miR-1233-5p in Patients with Alzheimer’s Pathologic Change with Mild Cognitive Impairment is Associated with Aβ-Induced Platelet Activation via P-Selectin. J Clin Med. 2020;9(6):1642.
101. Zhu YG, Shi MM, Monsel A, Dai CX, Dong X, Shen H, et al. Nebulized exosomes derived from allogenic adipose tissue mesenchymal stromal cells in patients with severe COVID-19: a pilot study. Stem Cell Res Ther. 2022;13(1):220.
102. Taghiyar L, Jahangir S, Khozaei Ravari M, Shamekhi MA, Eslaminejad MB. Cartilage Repair by Mesenchymal Stem Cell-Derived Exosomes: Preclinical and Clinical Trial Update and Perspectives. Adv Exp Med Biol. 2021;1326:73-93.
103. Rezaie J, Feghhi M, Etemadi T. A review on exosomes application in clinical trials: perspective, questions, and challenges. Cell Commun Signal. 2022;20(1):145. Published 2022 Sep 19.
104. Johnson J, Law SQK, Shojaee M, Hall AS, Bhuiyan S, LIm MBL, et al. First-in-human clinical trial of allogeneic, platelet-derived extracellular vesicles as a potential therapeutic for delayed wound healing. J Extracell Vesicles. 2023;12(7):e12332.
105. Skuratovskaia D, Vulf M, Khaziakhmatova O, Malashchenko V, Komar A, Shunkin E, et al. Exosome limitations in the treatment of inflammatory diseases. Curr Pharm Des. 2021;27(28):3105-3121.
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