Paper strip sensor based on γ-fe2O3@prussian blue nanozyme for H2O2 detection

Document Type : Research Paper

Authors

1 Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences (TUMS), Tehran, Iran

2 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Abstract

Objective(s): High levels of hydrogen peroxide (H2O2) induce oxidative stress in physiological environments. Elevation of H2O2 levels in semen can be a reason for male infertility, by causing protein and enzyme denaturation, lipid peroxidation, and DNA damage. Oxidative stress can affect sperm features, such as viability, motility, and fertilization potential. Although nanozymes are widely used to detect H2O2 using different techniques, monitoring of H2O2 in physiological fluids remains a challenge that has not been studied extensively. We report on a non-enzymatic paper strip based on γ-Fe2O3@Prussian blue nanoparticles (γ-Fe2O3@PB NPs) and their performance for H2O2 detection in buffer and seminal plasma. 
Materials and Methods: γ-Fe2O3 NPs were synthesized using chemical coprecipitation method and were then coated with PB. γ-Fe2O3@PB NPs were characterized using ultraviolet-visible spectroscopy (UV-vis), dynamic light scattering (DLS), X-ray diffraction (XRD), and transmission electron microscopy (TEM). The results confirmed formation of relatively monodisperse and approximately 71 nm γ-Fe2O3@PB NPs. The peroxidase-like activities of γ-Fe2O3 NPs and γ-Fe2O3@PB NPs were measured using UV-visible spectroscopy. 
Results: The results demonstrated that the catalytic activity of  γ-Fe2O3@PB NPs was higher than that of γ-Fe‌2‌O3 NPs. The concentrations of γ-Fe2O3@PB NPs and TMB, immobilized on paper strips, were optimized. The detection limit of the constructed lateral flow assays (LFA) for H2O2 in acetate buffer was 50.0 µM. Citric acid and ascorbic acid, as common components in semen, showed interference with the performance of paper strips. The γ-Fe2O3@PB NPs-based paper strip could detect H2O2 spiked in human seminal plasma in 20 min with a detection limit of 750.0 µM. 
Conclusion: The colorimetric detection of H2O2 on paper strips was successful and quantification of the results was possible with the help of a cell phone, which makes it a breakthrough in quantitative rapid tests. 

Keywords

References

1. Yu Y, Pan M, Peng J, Hu D, Hao Y, Qian Z. A review on recent advances in hydrogen peroxide electrochemical sensors for applications in cell detection. Chin Chem Lett. 2022; 33(9): 4133-4135.
2. Manickam P, Vashist A, Madhu S, Sadasivam M, Sakthivel A, Kaushik A, et al., Gold nanocubes embedded biocompatible hybrid hydrogels for electrochemical detection of H2O2. Bioelectrochemistry. 2020; 131: 107373.
3. Peng L, Guo H, Wu N, Wang M, Hui Y, Ren H, et al. Fluorescent sensor based on bismuth metal-organic frameworks (Bi-MOFs) mimic enzyme for H2O2 detection in real samples and distinction of phenylenediamine isomers. Talanta. 2024; 272: 125753.
4. Blanco E, Vázquez L, Pozo M, Roy R, Petit-Domínguez M D, Quintana C, et al. Evaluation of oxidative stress: nanoparticle-based electrochemical sensors for hydrogen peroxide determination in human semen samples. Bioelectrochemistry. 2020; 135: 107581.
5. Ransy C, Vaz C, Lombès A, Bouillaud F. Use of H2O2 to cause oxidative stress, the catalase issue. Int J Mol Sci. 2020; 21(23): 9149.
6. Wang Z, Hong Y, Li J, Liu J, Jiang H, Sun L. Upconversion luminescent sensor for endogenous H2O2 detection in cells based on the inner filter effect of coated silver layer. Sens Actuators B Chem. 2023; 376: 132936.
7. Alsulami T, Alzahrani A. Enhanced nanozymatic activity on rough surfaces for H2O2 and tetracycline detection. Biosensors. 2024; 14(2): 106.
8. Gökçal B, Kip C, Şahinbaş D, Çelik E, Tuncel A, Silica microspheres functionalized with the iminodiacetic acid/copper (II) complex as a peroxidase mimic for use in metal affinity chromatography-based colorimetric determination of histidine-tagged proteins. Microchim Acta. 2020; 187: 1-9.
9. Shafa M, Ahmad I, Hussain S, Asif M, Pan Y, Zairov R, et al., Ag-Cu nanoalloys: An electrochemical sensor for H2O2 detection. Surf Interfaces. 2023; 36: 102616.
10. Li B, Wang R, Li G, Shen Q, Zou L, NiCoMnS/rGO nanocomposite for enzyme-free and ultrasensitive electrochemical catalysis of hydrogen peroxide and glucose. Microchem J. 2024; 199: 109947.
11. Zhang W, Lan Y, Chai D-F, Lv J, Dong G, Guo D, A novel “On-Off” colorimetric sensor for ascorbic acid and hydrogen peroxide based on peroxidase activity of CeO2/Co3O4 hollow nanocubes. J Mol Struct. 2024; 1302: 137507.
12. Abedalwafa MA, Li Y, Ni C, Yang G, Wang L, Non-enzymatic colorimetric sensor strip based on melamine-functionalized gold nanoparticles assembled on polyamide nanofiber membranes for the detection of metronidazole. Anal Methods. 2019; 11(29): 3706-3713.
13. Ghasemi F, Fahimi-Kashani N, Bigdeli A, Alshatteri A H, Abbasi-Moayed S, Al-Jaf S H, et al., Paper based optical nanosensors–A review. Anal Chim Acta. 2023; 1238: 340640.
14. Hu J, Wang S, Wang L, Li F, Pingguan-Murphy B, Lu T J, et al., Advances in paper-based point-of-care diagnostics. Biosens Bioelectron. 2014; 54: 585-597.
15. Dkhar D S, Kumari R, Malode S J, Shetti N P, Chandra P, Integrated lab-on-a-chip devices: Fabrication methodologies, transduction system for sensing purposes. J Pharm Biomed Anal. 2023; 223: 115120.
16. Atabakhsh S, Abbasali HH, and Ashtiani SJ, Thermally programmable time delay switches for multi-step assays in paper-based microfluidics. Talanta. 2024; 271: 125695.
17. Shirshahi V and Afrapoli ZB, Lateral flow assay with green nanomaterials. Comprehensive Analytical Chemistry. 2023; 105: 301-330.
18. Fung, KK, Chan CPY, Renneberg R. Development of enzyme-based bar code-style lateral-flow assay for hydrogen peroxide determination. Anal Chim Acta. 2009; 634(1): 89-95.
19. Chi Z, Wang Q, and Gu J, Recent advances of colorimetric sensors based on nanozymes with peroxidase-like activity. Analyst. 2022; 148(3): 487-506.
20. Tan W, Yao G, Yu H, He Y, Lu M, Zou T, et al., Ultra-trace Ag doped carbon quantum dots with peroxidase-like activity for the colorimetric detection of glucose. Food Chem. 2024; 447: 139020.
21. Niu X, Cheng N, Ruan X, Du D, Lin Y, Nanozyme-based immunosensors and immunoassays: recent developments and future trends. J Electrochem Soc. 2020; 167(3): 037508.
22. Jabiyeva N, Çakıroğlu B, and Özdemir A, The peroxidase-like activity of Au NPs deposited inverse opal CeO2 nanozyme for rapid and sensitive H2O2 sensing. J Photochem Photobiol A Chem. 2024; 452: 115576.
23. Song C, Ding W, Zhao W, Liu H, Wang J, Yao Y, et al., High peroxidase-like activity realized by facile synthesis of FeS2 nanoparticles for sensitive colorimetric detection of H2O2 and glutathione. Biosens Bioelectron. 2020; 151: 111983.
24. Çakıroğlu B, Graphene quantum dots on TiO2 nanotubes as a light-assisted peroxidase nanozyme. Microchim Acta. 2024; 191(5): 1-10.
25. Marvi P K, Ahmed S R, Das P, Ghosh R, Srinivasan S, Rajabzadeh A R, Prunella vulgaris-phytosynthesized platinum nanoparticles: Insights into nanozymatic activity for H2O2 and glutamate detection and antioxidant capacity. Talanta. 2024; 274: 125998.
26. Dutta S, Majzoub A, and Agarwal A, Oxidative stress and sperm function: A systematic review on evaluation and management. Arab J Urol. 2019; 17(2): 87-97.
27. Kullisaar T, Türk S, Kilk K, Ausmees K, Punab M, Mändar R, Increased levels of hydrogen peroxide and nitric oxide in male partners of infertile couples. Andrology. 2013; 1(6): 850-858.
28. Promsuwan K, Soleh A, Saisahas K, Saichanapan J, Thiangchanya A, Phonchai A, et al., Micro-colloidal catalyst of palladium nanoparticles on polyaniline-coated carbon microspheres for a non-enzymatic hydrogen peroxide sensor. Microchem J. 2021; 171: 106785.
29. Sun Y-k, Ma M, Zhang Y, Gu N, Synthesis of nanometer-size maghemite particles from magnetite. Colloids Surf A Physicochem Eng Asp. 2004; 245(1-3): 15-19.
30. Zhang X-Q, Gong S-W, Zhang Y, Yang T, Wang C-Y, Gu N, Prussian blue modified iron oxide magnetic nanoparticles and their high peroxidase-like activity. J Mater Chem. 2010; 20(24): 5110-5116.
31. Zhang W, Niu X, Li X, He Y, Song H, Peng Y, et al., A smartphone-integrated ready-to-use paper-based sensor with mesoporous carbon-dispersed Pd nanoparticles as a highly active peroxidase mimic for H2O2 detection. Sens Actuators B Chem. 2018; 265: 412-420.
32. Sikirzhytskaya A, Sikirzhytski V, Pérez-Almodóvar L, Lednev I K, Raman spectroscopy for the identification of body fluid traces: semen and vaginal fluid mixture. Forensic Chem. 2023; 32: 100468.
33. Videla E, Blanco A M, Galli M E, FERNÁNDEZ-COLLAZO E, Human seminal biochemistry: fructose, ascorbic acid, citric acid, acid phosphatase and their relationship with sperm count. Andrologia. 1981; 13(3): 212-214.
34. Pyrasch M and Tieke B, Electro-and photoresponsive films of Prussian blue prepared upon multiple sequential adsorption. Langmuir. 2001; 17(24): 7706-7709.
35. Estelrich J and Busquets M A, Prussian blue: a nanozyme with versatile catalytic properties. Int J Mol Sci. 2021; 22(11): 5993.
36. Dutta A K, Maji S K, Srivastava D N, Mondal A, Biwas P, Paul P, et al., Peroxidase-like activity and amperometric sensing of hydrogen peroxide by Fe2O3 and Prussian Blue-modified Fe2O3 nanoparticles. J Mol Catal A Chem. 2012; 360: 71-77.
37. Misro M M, Choudhury L, Upreti K, Gautam D, Chaki S P, Mahajan A S, et al., Use of hydrogen peroxide to assess the sperm susceptibility to oxidative stress in subjects presenting a normal semen profile. Int J Androl. 2004; 27(2): 82-87.
38. Aslanzadeh S, Ishola M M, Richards T, Taherzadeh M J, An overview of existing individual unit operations. Biorefineries, 2014; 3-36.
39. Shu X, Chang Y, Wen H, Yao X, Wang Y, Colorimetric determination of ascorbic acid based on carbon quantum dots as peroxidase mimetic enzyme. RSC Adv. 2020; 10(25): 14953-14957.
40. Fan X, Bao Y, Chen Y, Wang X, On S L W, Wang J, Synthesis of β-Cyclodextrin@ gold nanoparticles and its application on colorimetric assays for ascorbic acid and Salmonella based on peroxidase-like activities. Biosensors. 2024; 14(4): 169.
41. Jia S, Zhang X, Yuan F, Xia T, Colorimetric test paper for H2O2 determination based on peroxidase-like activity of an AuFe/ZIF-8-graphene composite. ChemistrySelect. 2022; 7(43): e202202984.
42. Tesfaye T and Hussen A, Microfluidic paper-based analytical device (µPAD) fabricated by wax screen printing technique for the determination of nitrite and nitrate ion in water samples. Microfluid Nanofluidics. 2022; 26(3): 22.
Nanomedicine Journal
Volume 12, Issue 2
April 2025
Pages 279-288

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