1. Urish KL, Cassat JE. Staphylococcus aureus osteomyelitis: bone, bugs, and surgery. Infect Immun. 2020; 88(7).
2. Wu S, Wu B, Liu Y, Deng S, Lei L, Zhang H. Mini review therapeutic strategies targeting for biofilm and bone infections. Front Microbiol. 2022; 13.
3. Hofstee MI, Muthukrishnan G, Atkins GJ, Nizet V, Bonhoeffer S, Zinkernagel AS, et al. Current concepts of osteomyelitis. Am J Pathol. 2020; 190(6): 1151–1163.
4. Mouzopoulos G, Kanakaris NK, Kontakis G, Obakponovwe O, Townsend R, Giannoudis PV. Management of bone infections in adults: the surgeon's and microbiologist's perspectives. Injury. 2011; 42(Suppl 5): S18–S23.
5. Masters EA, Ricciardi BF, Bentley KL de M, Moriarty TF, Schwarz EM, Muthukrishnan G. Skeletal infections: microbial pathogenesis, immunity and clinical management. Nat Rev Microbiol. 2022; 20.
6. Zapata D, Higgs J, Wittholt H, Chittimalli K, Brooks AE, Mulinti P. Nanotechnology in the diagnosis and treatment of osteomyelitis. Pharmaceutics. 2022; 14(8): 1563.
7. Stobba BK, Kasprzak M. Fabrication of nanoparticles for bone regeneration: new insight into applications of nanoemulsion technology. J Mater Chem B. 2021; 9: 5221–5244.
8. Ielo I, Calabrese G, De Luca G, Conoci S. Recent advances in hydroxyapatite-based biocomposites for bone tissue regeneration in orthopedics. Int J Mol Sci. 2022; 23(17): 9721.
9. Hatami Kaleshtari A, Farjaminejad S, Hasani M, Farjaminejad R, Gharibshahian M, Zafari M, et al. The critical role of nano-hydroxyapatites as an advanced scaffold in drug delivery towards efficient bone regeneration: recent progress and challenges. Carbohydr Polym Technol Appl. 2025; 9: 100692.
10. Jamarun N, Afriska LN, Wellia DV, Arief S, Aziz H, Ismail A, et al. Investigation of the antibacterial activity of synthesized hydroxyapatite Sr-doped nanocomposite. J Appl Pharm Sci. 2024; 14(9).
11. Kyeremeh SP, Asimeng BO, Paemka L, Kojo MA, Annan E, Tiburu EK. Antibacterial activity of microwave hydroxyapatite and cellulose blend. Oxf Open Mater Sci. 2024; 4(1): itae010.
12. Zhao C, Liu W, Zhu M, Wu C, Zhu Y. Bioceramic based scaffolds with antibacterial function for bone tissue engineering: a review. Bioact Mater. 2022; 18.
13. Yadav U, Verma V. Halloysite nanoclay reinforced hydroxyapatite porous scaffold for hard tissue regeneration. J Mech Behav Biomed Mater. 2023; 140: 105626.
14. Wang C, Guo T, Gong Y, Xu L, Zhang J, Chen Y, et al. Novel polydopamine/halloysite nanotube-reinforced brushite calcium phosphate cement for bone regeneration with synergistic regulation of mechanical/osteogenic capacity. Mater Adv. 2025; 6: 1959–1964.
15. Same S, Akbari Nakhjavani S, Samee G, Agbolaghi S, Jahanban-Esfahlan R, Omidi Y, et al. Halloysite clay nanotube in regenerative medicine for tissue and wound healing. Ceram Int. 2022; 48: 31065–31079.
16. Ahmad R, Singh A, Purba A, Rashidinejad A. Novel rutin casein composites as functional dry ingredients for the delivery of high concentration of rutin in dairy beverages: in vitro bioaccessibility, cytotoxicity, absorption, and intestinal barrier integrity. Food Hydrocoll. 2025; 170: 111735.
17. Akash SR, Tabassum A, Aditee LM, Rahman A, Hossain MI, Hannan MA, et al. Pharmacological insight of rutin as a potential candidate against peptic ulcer. Biomed Pharmacother. 2024; 177: 116961.
18. Singh A, Kundu M, Chatterjee S, Das S, Ghosh S, Chattopadhyay N, et al. Synthesis of rutin loaded nanomagnesia as a smart nanoformulation with significant antibacterial and antioxidant properties. Inorg Chem Commun. 2022; 140: 109492.
19. Ivanov M, Novović K, Malesevic M, Dinic M, Stojković D, Jovcic B, et al. Polyphenols as inhibitors of antibiotic resistant bacteria: mechanisms underlying rutin interference with bacterial virulence. Pharmaceuticals. 2022; 15(3): 385.
20. Esnaashari F, Zahmatkesh H. Antivirulence activities of rutin loaded chitosan nanoparticles against pathogenic Staphylococcus aureus. BMC Microbiol. 2024; 24(1).
21. Liu XW, Ma B, Zi Y, Xiang LB, Han TY. Effects of rutin on osteoblast MC3T3-E1 differentiation, ALP activity and Runx2 protein expression. Eur J Histochem. 2021; 65(1): 3195.
22. Hyun H, Park H, Jeong J, Kim J, Kim H, Oh HI, et al. Effects of watercress containing rutin and rutin alone on the proliferation and osteogenic differentiation of human osteoblast like MG-63 cells. Korean J Physiol Pharmacol. 2014; 18(4): 347.
23. Abdel-Naim AB, Alghamdi AA, Algandaby MM, Al-Abbasi FA, Al-Abd AM, Eid BG, et al. Rutin isolated from Chrozophora tinctoria enhances bone cell proliferation and ossification markers. Oxid Med Cell Longev. 2018; 2018: 5106469.
24. Vijayalekha A, Sridhar H, Srinivasan S, Anumaiya V, Anandasadagopan SK, Pandurangan AK. Integrative network pharmacology and experimental validation of rutin-infused collagen–hydroxyapatite scaffold for promoting osteochondral regeneration. 3 Biotech. 2025; 15(9): 290.
25. Dadgar A, Maleki Dizaj S, Alizadeh Oskoee P, Pournaghi-Azar F, Jafari Navimipour E. Gelatin nanofibrous scaffolds containing rutin nanoparticles: physicochemical properties, antibacterial action, and anti-inflammatory effect on dental pulp stem cells. BMC Oral Health. 2025; 25: 1129.
26. Kyriacou H, Kamaraj A, Khan WS. Developments in antibiotic-eluting scaffolds for the treatment of osteomyelitis. Appl Sci. 2020; 10: 2244.
27. Memar MY, Dalir Abdolahinia E, Yekani M, Kouhsoltani M, Sharifi S, Maleki Dizaj S, et al. Preparation of rutin-loaded mesoporous silica nanoparticles and evaluation of its physicochemical, anticancer, and antibacterial properties. Mol Biol Rep. 2022; 50: 203–213.
28. Hussain H, L SR, Ahmad S, Abd Razak MF, Wan Mustapha WA, Zainol MK, et al. Determination of cell viability using acridine orange/propidium iodide dual spectrofluorometry assay. Cogent Food Agric. 2019; 5(1): 1582398.
29. Kalaiyarasi J, Meenakshi S, Gopinath SCB, Pandian K. Mediator free simultaneous determination of acetaminophen and caffeine using a glassy carbon electrode modified with a nanotubular clay. Microchim Acta. 2017; 184: 4485–4494.
30. Manoj M, Mangalaraj D, Ponpandian N, Viswanathan C. Core–shell hydroxyapatite/Mg nanostructures: surfactant free facile synthesis, characterization and their in vitro cell viability studies against leukaemia cancer cells (K562). RSC Adv. 2015; 5: 48705–48711.
31. Barot T, Rawtani D, Kulkarni P. Physicochemical and biological assessment of silver nanoparticles immobilized halloysite nanotubes-based resin composite for dental applications. Heliyon. 2020; 6(3): e03601.
32. Luo S, Fu Y, Ye J, Liu C. Encapsulation of rutin in protein nanoparticles by pH-driven method: impact of rutin solubility and mechanisms. J Sci Food Agric. 2024; 104(3): 1804–1812.
33. Li C, Chen L, McClements DJ, Peng X, Xu Z, Meng M, et al. Preparation and characterization of rutin-loaded zein–carboxymethyl starch nanoparticles. Foods. 2022; 11(18): 2827.
34. Khan S, Kumar V, Roy P, Kundu PP. TiO2 doped chitosan/hydroxyapatite/halloysite nanotube membranes with enhanced mechanical properties and osteoblast like cell response for application in bone tissue engineering. RSC Adv. 2019; 9(68): 39768–39779.
35. Rance GA, Marsh DH, Bourne SJ, Reade TJ, Khlobystov AN. van der Waals interactions between nanotubes and nanoparticles for controlled assembly of composite nanostructures. ACS Nano. 2010; 4(8): 4920–4928.
36. Zia I, Mirza S, Jolly R, Rehman S, Owais M, Shakir M. Trigonella foenum graecum seed polysaccharide coupled nano hydroxyapatite-chitosan: a ternary nanocomposite for bone tissue engineering. Int J Biol Macromol. 2018; 124: 88–101.
37. Zheng J, Wu F, Li H, Liu M. Preparation of bioactive hydroxyapatite@halloysite and its effect on MC3T3-E1 osteogenic differentiation of chitosan film. Mater Sci Eng C. 2019; 105: 110072.
38. Hong D, Chen HX, Yu HQ, Liang Y, Wang C, Lian QQ, et al. Morphological and proteomic analysis of early stage of osteoblast differentiation in osteoblastic progenitor cells. Exp Cell Res. 2010; 316(14): 2291–2300.
39. Hong Y, Boiti A, Vallone D, Foulkes NS. Reactive oxygen species signaling and oxidative stress: transcriptional regulation and evolution. Antioxidants. 2024; 13(3): 312.
40. Li N, Oquendo E, Capaldi RA, Robinson JP, He YD, Hamadeh HK, et al. A systematic assessment of mitochondrial function identified novel signatures for drug induced mitochondrial disruption in cells. Toxicol Sci. 2014; 142(1): 261–273.
41. Reffitt DM, Ogston N, Jugdaohsingh R, Cheung HFJ, Evans BAJ, Thompson RPH, et al. Orthosilicic acid stimulates collagen type I synthesis and osteoblastic differentiation in human osteoblast like cells in vitro. Bone. 2003; 32(2): 127–135.
42. Jin Y, Liu X, Liu H, Chen S, Gao C, Ge K, et al. Oxidative stress induced apoptosis of osteoblastic MC3T3-E1 cells by hydroxyapatite nanoparticles through lysosomal and mitochondrial pathways. RSC Adv. 2017; 7(21): 13010–13018.
43. Porter AE, Patel N, Skepper JN, Best SM, Bonfield W. Comparison of in vivo dissolution processes in hydroxyapatite and silicon substituted hydroxyapatite bioceramics. Biomaterials. 2003; 24(25): 4609–4620.
44. Li Q, Wu Y, Zhang Y, Mao J, Zhang Z. Enhancing osteogenic differentiation of MC3T3-E1 cells during inflammation using UPPE/β-TCP/TTC composites via the Wnt/β-catenin pathway. RSC Adv. 2024; 14(3): 1527–1537.
45. Wu Y, Wang Y, Chen F, Wang B. Loading rutin on surfaces by the layer-by-layer assembly technique to improve the oxidation resistance and osteogenesis of titanium implants in osteoporotic rats. Biomed Mater. 2024; 19(4): 045011.
46. Xiao S, Wang M, Wang L, Zhu Y. Environment friendly synthesis of trace element Zn, Sr, and F codoping hydroxyapatite with non cytotoxicity and improved osteoblast proliferation and differentiation. Biol Trace Elem Res. 2018; 185(1): 148–161.
47. Hu S, Meng Z, Zhou J, Wang W, Liu Y, Liu W, et al. Enhanced attachment and collagen type I deposition of MC3T3-E1 cells via electrohydrodynamic printed sub microscale fibrous architectures. Int J Bioprint. 2022; 8(2): 514.
48. Valdez-Salas B, Castillo-Uribe S, Beltran-Partida E, Curiel-Alvarez M, Perez-Landeros O, Guerra-Balcazar M, et al. Recovering osteoblast functionality on TiO2 nanotube surfaces under diabetic conditions. Int J Nanomedicine. 2022; 17: 5469–5488.
