Nanomedicine Journal

Nanomedicine Journal

Selenium nanoparticle-loaded nanofibers accelerate in vivo wound closure in a rat model

Articles in Press

Document Type : Research Paper

Authors
Razieh Ghorbani 1
Safieh Boroumand 2
Raheleh Faridi Majidi 3
Seyed Mohsen Ahmadi Tafti 4
Amir Marashi 5
Mohammad Mehdi Khani 6
1 Student Research Committee, Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Research Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran
3 Biomaterials Laboratory, Division of Biomedical Engineering, Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
4 Colorectal Surgery Research Center, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran, Iran
5 Shahid Beheshti University of Medical Sciences
6 Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
10.22038/nmj.2026.95562.2419
Abstract
Objective(s): The inefficiency of conventional antibiotics has led to the emergence of multidrug-resistant (MDR) pathogens, making it worthwhile to develop alternative antibacterial agents to overcome this challenge. Infection at the wound site may lead to wound dehiscence, resulting in increased patient morbidity and higher healthcare costs. Nanoparticle-based approaches offer a promising strategy for controlling wound infections by enabling targeted delivery of antimicrobial agents, disrupting bacterial cell membranes and biofilms, and supporting tissue regeneration, while potentially helping to limit the development of antimicrobial resistance. Interestingly, it has been revealed that drug-resistant pathogens can be limited by Selenium nanoparticles (SeNPs).
Materials and Methods: This study aimed to synthesize and evaluate selenium nanoparticle-loaded nanofibers for wound dressing. SeNPs were green-synthesized and characterized by DLS, TEM, and EDX. The nanoparticle-loaded nanofibers were fabricated by electrospinning technique and characterized morphologically and chemically by SEM and ATR-FTIR. The in vitro safety of these nanoparticle-loaded nanofibers was demonstrated in the MTT assay on HU-02 fibroblast cells, while the bacterial viable cell count was used to investigate the antibacterial assessment against two strains of Staphylococcus aureus and Escherichia coli. In vivo efficiency was performed on Wistar Rats.
Results: Selenium-loaded nanofibers (129 ± 30.21 nm) showed antibacterial activity with no in vitro toxicity. An in vivo full-thickness wound model confirmed enhanced wound healing in histological evaluations.
Conclusion: This study revealed the potential of SeNPs-loaded nanofibers for an efficient antibacterial wound dressing.
Keywords
Nanoparticles
Selenium
Wound dressing
Electrospun nanofibers
Chitosan
Subjects
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Nanomedicine Journal

Articles in Press, Accepted Manuscript
Available Online from 26 October 2026