Antimicrobial and cytotoxicity effect of silver nanoparticle synthesized by Croton bonplandianum Baill. leaves

Document Type : Research Paper


1 Department of Biotechnology, Panskura Banamali College; East Midnapore; West Bengal; INDIA

2 Radiation Biology Division,UGC-DAE CSR, Kolkata Centre, Sector III, LB-8 Bidhan Nagar


Objective(s): For the development of reliable, ecofriendly, less expensive process for the synthesis of silver nanoparticles and to evaluate the bactericidal, and cytotoxicity properties of silver nanoparticles synthesized from root extract of Croton bonplandianum, Baill.
Materials and Methods: The synthesis of silver nanoparticles by plant part of Croton bonplandianum was carried out.  The formation of nanoparticles was confirmed by Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), XRD and UV-Vis spectrophotometric analysis.  The biochemical properties were assayed by antibacterial study, cytotoxicity assay using cancer cell line. 
Results: The formation of silver nanoparticles was confirmed by UV-VIS spectroscopic analysis which showed absorbance peak at 425 nm.  X-ray diffraction photograph indicated the face centered cubic structure of the synthesized AgNPs.  TEM has displayed the different dimensional images of biogenic silver nanoparticles with particle size distribution ranging from 15-40 nm with an average size of 32 nm. Silver particles are spherical in shape, clustered.  The EDX analysis was used to identify the elemental composition of synthesized AgNPs. Antibacterial activity of the synthesized AgNPs against three Gram positive and Gram negative bacteria strains like Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa carried out showed significant zones of inhibition. The cytotoxicity study by AgNPS also showed cytotoxicity on ovarian cancer cell line PA-1 and lung epithelial cancer cell line A549. 
Conclusion: The present study confirms that the AgNPs have great promise as antibacterial, and anticancer agent.


[1]  Vert M, Doi Y, Hellwich KH, Hess M, Hodge P, Kubisa P, Rinaudo M, Schué F. Terminology for biorelated polymers and applications (IUPAC Recommendations 2012). Pure Appl Chem. 2012; 84(2): 377-410.
[2]  Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007; 3(1): 95-101.
[3]  Panácek A1, Kolár M, Vecerová R, Prucek R, Soukupová J, Krystof V, Hamal P, Zboril R, Kvítek L. Antifungal activity of silver nanoparticles against Candida spp. Biomaterials. 2009; 30(31): 6333-6340.
[4]  De Jong WH, Borm P. Drug delivery and nanoparticles: Applications and hazards. Int J Nanomedicine. 2008; 3(2): 133-149.
[5]  Bhattacharya D, Gupt RK. Nanotechnology and potential of microorganisms. Crit Rev Biotechnol. 2005; 25: 199-204.
[6]  Siulvaenrg H, Nuann DA, Oq D, Alrut Iycl DSP, Yoavnelg SN. Bcioinsynnatmheosmisu Mofcamphora leaf. Nanotechnology. 2007; 18: 103-105.
[7]  Wijnhoven SWP, Peijnenburg WJGM, Herberts CA, Hagens WI, Oomen AG, Heugens EHW, et al. Nano-silver: a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology. 2009; 3: 109-138.
[8]  Guzmán MG, Dille J, Godet S. Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity. Int J Chem Biol Eng. 2009; 2: 104-111.
[9]  Navaladian S, Viswanathan B, Viswanath RP, Varadarajan TK. Thermal decomposition as route for silver nanoparticles. Nanoscale Res Lett. 2007; 2: 44-48.
[10] Sastry M, Ahmad A, Khan MI, Kumar R. Biosynthesis of metal nanoparticles using fungi and actinomycete. Curr Sci. 2003; 85: 162-170.
[11] Reddy KR. Folk medicines from Chittor District,Andra Pradesh,used in treatment of jaundice. Pharm Biol. 1995; 26(3): 137-140.
[12] Bapuji WJ, Ratnam VS. Leaflets. Ethnobotany. 2009; 13: 388-389.
[13] Chandel KPS, Shukla G, Sharma N. Biodiversity in medicinal and aromatic plants in India: Conservation and utilization: In: New Delhi: National Bureau of Plant Genetic Resources; 1996, p.361.                                                                               
[14] Rajakaruna N, Harris CS, Towers GHN. Antimicrobial activity of plants collected from serpentine outcrops in Sri Lanka. Pharm Biol. 2002; 40(3): 235-244.
[15] Rizk AM. The chemical constituents and economic plants of Euphorbiaceae. Bot J Linn Soc. 1987; 94(1-2): 293-326.
[16] Phillipson JD. A matter of some sensitivity. Phytochemistry. 1995; 38(6): 1319-1343.
[17]  Dosuma OO, Nwosu FO, Nwogu CD. Antimicrobial studies and phytochemical screening of extracts of Hyphaenethebaica (Linn) Mart fruits. Int J Trop Med. 2006; 1(4): 186–222.
[18] Gavhane AJ, Padmanabhan P, Kamble SP, Jangle SN. Synthesis of silver nanoparticles using extract of neem leaf and triphala and evaluation of their antimicrobial activities. Int J Pharm Bio Sci. 2012; 3(3): 88-100.
[19] Sinha SN, Paul D, Halder N, Sengupta D, Patra SK. Green synthesis of silver nanoparticles using fresh water green alga Pithophora oedogonia (Mont.) Wittrock and evaluation of their antibacterial activity. Appl Nanosci. 2015; 5: 703-709.
[20] Kumari AS, Venkatesham M, Ayodhya D, Veerabhadram G. Green synthesis, characterization and catalytic activity of palladium nanoparticles by xanthan gum. Appl Nanosci.  2015; 5: 315-320.
[21] Annamalai J, Nallamuthu T. Green synthesis of silver nanoparticles: characterization and determination of antibacterial potency. Appl Nanosci. 2015; 1-7.
[22] Furno F, Morley KS, Wong B, Sharp BL, Arnold PL, Howdle SM, et al. Silver nanoparticles and polymeric medical devices. J Antimicrob Chemother. 2004; 54: 1019-1024.
[23] Nathan P, Law EJ, Murphy DF, MacMillan BG. A laboratory method for selection of topical antimicrobial agents to treat infected burn wounds. Burns. 1978; 4: 177-178.
[24] Khanra K, Roy A, Bhattacharyya N. Evaluation of Antibacterial Activity and Cytotoxicity of Green Synthesized Silver Nanoparticles Using Hemidesmus Indicus R. Br. American Journals of Nanoscience and Nanotechnology Research. 2013; 1: 1-6.
[25] Malthankar GV, White BK, Bhusan A, Daniels CK, Rodnick KJ, Lai JCK. Differential lowering by manganese treatment of activities of glycolytic and tricarboxylic acid (TCA) cycle enzymes investigated in neuroblastoma and astrocytoma cells is associated with manganese-induced cell death. Neurochem Res. 2004; 29: 707-717.
[26] Bar H, Bhui DK, Gobinda SP, Sarkar PM, Pyne S, Misra A. Green synthesis of silver nanoparticles using seed extract of Jatropha curcas. Physicochem Eng Aspects. 2009: 348: 212–216.
[27] Ahmad N, Sharma S, Alam MK, Singh VN, Shamsi SF, Mehta BR, Fatma A. Rapid synthesis of silver nanoparticles using dried medicinal plant of basil. Colloids Surf B Biointerfaces. 2010: 81(1): 81–86.
[28] Parasad KS, Pathak D, Patel A, Dalwadi P, Prasad R, Patel P, Selvaraj K. Biogenic synthesis of silver nanoparticles using Nicotiana tobaccum leaf extract and study of their antimicrobial effect. Afr J Biotechnol. 2011; 10: 8122–8130.
[29] Singhal G, Bhavesh R, Kasariya K, Sharma AR, Singh RP. Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening ts antimicrobial activity. J Nanopart Res. 2011;13: 2981–2988.
[30] Logeswari P, Silambarasan S, Abraham J. Synthesis of silver nanoparticles using plants extract and analysis of their antimicrobial property. J Saudi Chem Soc. 2012; 19(3): 311-317.
[31] Geethalakshmi E, Sarada DV. Synthesis of plant-mediated silver nanoparticles using Trianthema decandra extract and evaluation of their anti microbial activities. Int J Eng Sci Tech. 2010; 2: 970–975.
[32] Leela A, Vivekanandan M. Tapping the unexploited plant resources for the synthesis of silver nanoparticles. Afr J Biotechnol. 2008; 7: 3162–5315.
[33] Bankar A, Joshi B, Kumar AR, Zinjarde S. Banana peel extract mediated novel route for synthesis of silver nanoparticles. Colloid Surf A Physicochem Eng Aspect. 2009; 368: 58–63.
[34] Dwivedi AD, Gopal K. Biosynthesis of silver and gold nanoparticles using Chenopodium album leaf extract. Colloid Surf A Physicochem Eng Aspect. 2010; 369: 27–33.
[35] Saxena A, Tripathi RM, Zafar F, Singh P.  Green synthesis of silver nanoparticles using aqueous solution of Ficus benghalensis leaf extract and characterization of their antimicrobial activity. Mater Letters. 2012; 67: 91–94.
[36] Awwad AM, Salem NM. Green synthesis of silver nanoparticles by mulberry leaves extract. Nanosci Nanotechno. 2012; 2: 125–128.
[37] Nishanta R, Harris CS, Towers GHN. Antimicrobial activity of plants collected from serpentine outcrops in Sri Lanka. Pharmaceutical Biology. 200; 40(3): 235–244.
[38] Chaudhuri AB. Endangered Medicinal Plants. Delhi: Daya Publishing House 2007.
[39] Bhakat RK, Sen UK. Ethnomedicinal plant conservation through sacred groves. Tribes and Tribals. 2008; 2: 55-58.R.
[40] R. Sathyavathi, M.B.M. Krishna, S.V. Rao, R. Saritha, D.N. Rao.  Biosynthesis of silver nanoparticles using coriandrum sativum leaf extract and their application in nonlinear optics.  Adv Sci Lett. 2010; 3: 1–6.
[41] El-Batal, A.I., Hashem, A.A., Abdelbaky, N.M.: Gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity. Springerplus. 2013; 2: 1–10.
[42] Dibrov P, Dzoiba J, Gosink KK, Häse CC. Chemiosmotic mechanism of antimicrobial activity of Ag(+) in vibrio cholera. Antimicrob Agents Chemother. 2002; 46:2668–2770.
[43] Hamouda T, Myc A, Donovan B, Shih A, Reuter JD, Baker RJ. A novel surfactant nanoemulsion with a unique non-irritant topical antimicrobial against bacteria, enveloped viruses and fungi. Microbial Res. 2001; 156: 1-7.
[44] AshaRani PV, Low Kah Mun G, Hande MP, Valiyaveettil.  S. Cytotoxicity and genotoxicity of silver nanoparticles in human cells. ACS Nano. 2009; 3(2): 279-290.
[45] Khanra Kalyani, Panja Sudipta, Choudhuri Indranil, Chakraborty Anindita, Bhattacharyya Nandan. Evaluation of antibacterial activity and cytotoxicity of green synthesized silver nanoparticles using scorpia dulcis. Nano Biomed Eng. 2015; 7(3): 128-133.