Investigation of the effect of different parameters on the phase inversion temperature O/W nanoemulsions

Document Type: Research Paper


1 Young Researchers and Elite Club, East Tehran Branch, Islamic Azad University, Tehran, Iran

2 Department of Chemistry, faculty of science, Arak Branch, Islamic Azad University, Arak, Iran



Objective(s): Nanoemulsions are a kind of emulsions that can be transparent, translucent (size range 50-200 nm) or “milky” (up to 500 nm). Nanoemulsions are adequatly effective for transfer of active component through skin which facilitate the entrance of the active component . The transparent nature of the system and lack of the thickener and fluidity are among advantages of nanoemulsion.
Materials and Methods: In this study, a nanoemulsion of lemon oil in water was prepared by the phase inversion temperature (PIT) emulsification method in which the tween 40 was used as surfactant. The effect of concentration of NaCl in aqueous phase, pH and weight percent of surfactant and aqueous on the PIT and droplet size were investigated. Results: The results showed that with increasing of concentration of NaCl from 0.05 M to 1 M, PIT decrease from 72 to 50. The average droplet sizes, for 0.1, 0.5 and 1 M of NaCl in 25 ºC are 497.3, 308.1 and 189.9 nm, respectively and the polydispersity indexes are 0.348, 0.334 and 0.307, respectively.
Conclusion: Considering the characteristics of nanoemulsions such as being transparent, endurance of solution and droplet size can provide suitable reaction environment for polymerization process used in making hygienic and medical materials.


[1]  Ugelstad J, El-Aasser MS, and Vanderhoff JW. Emulsion polymerization: Initiation of polymerization in monomer droplets. J. Polym. Sci., Part C: Polym. Lett. 1973; 11(8): 503 -513.

[2]  El-Aasser MS, Lack CD, Choi YT, Min TI, Vanderhoff JW, Fowkes, FM. Interfacial aspects of miniemulsions and miniemulsion polymers. Colloid Surface. 1984; 12: 79-97.

[3]  El-Aasser MS, Lack CD, Vanderhoff JW, Fowkes F. The miniemulsification process-different form of spontaneous emulsification. Colloid Surface. 1988; 29: 103-118.

[4]  El-Aasser MS, Miller CM. Preparation of latexes using miniemulsions. In: Asua JM, ed., Polymeric Dispersions: Principles and Applications. Dordrecht, The Netherlands: Kluwer Academic Publishers. 1997; 335: 109-126.

[5]  Lovell PA, El-Aasser MS, Eds. Emulsion Polymerization and Emulsion Polymers. John Wiley and Sons: Chichester UK. 1997.

[6] Solans C, Kunieda H. Industrial Applications of Microemulsions. Marcel Dekker, Inc. Surfactant science series. New York 1997; 66: 406 pages.

[7]   Sole I, Maestro A, Pey CM, Gonzalez C, Solans C, Gutierrez JM. Nano-emulsions preparation by low energy methods in an ionic surfactant system. Colloids Surf., A: Physicochem. Eng. Aspects. 2006; 288(1-3): 138-143.

[8]  Nakajima H, Tomomasa S, Okabe M. Preparation of nanoemulsions. In proceeding of first Emulsion conference, Paris, France. EDS: Paris. 1993; 1: Paper No. 1-1-163.

[9]  Ee SL, Duan X, Liew J, Nguyen QD. Droplet size and stability of nano-emulsions produced by the temperature phase inversion method. Chem. Eng. J. 2008; 140(1-3): 626-631.

[10] Anton N, Gayet P, Benoit JP, Saulnier P. Nano-emulsions and nanocapsules by the PIT method: An investigation on the role of the temperature cycling on the emulsion phase inversion. Int. J. Pharm. 2007; 344(1-2): 44-52.

[11] E Dickinson. Hydrocolloids as emulsifiers and emulsion stabilizers. Food Hydrocolloids. 2009; 23(6): 1473–1482.

[12] Cheong JN, Tan CP, Che Man YB, Misran M. á-Tocopherol nanodispersions: Preparation, characterization and stability evaluation. J. Food Eng. 2008; 89(2): 204–209.

[13] Peter S, Given JR. Encapsulation of Flavors in Emulsions for Beverages. Curr. Opin. Colloid Interface Sci. 2009; 14(1): 43–47.

[14] Sanguansri P, Augustin MA. Nanoscale materials development–a food industry perspective. Trends Food Sci. Technol. 2006; 17(10): 547–556.

[15] Tan CP, Nakajima M. â-Carotene nanodispersions: preparation, characterization and stability evaluation. Food Chem. 2005; 92(4): 661–671.

[16] Ugwoke MI, Agu RU, Verbeke N, Kinget R. Nasal Mucoadhesive Drug Delivery: Background, Applications, Trends, and Future Perspectives. Adv. Drug Delivery Rev. 2005; 57(11): 1640- 1665.

[17] Pardridge WM. Non-Invasive Drug Delivery to Human Brain Using Endogenous Blood Brain Barrier Transport System. Pharm. Sci. Technol. Today. 1999; 2(2): 49-59.

[18] Asprion N, Blagov S, Ryll O, Welke R, Winterfeld A, Dittel A, Bortz M, Kufer KH, Burger J, Scheithauer A, Hasse H. Decision support for process development in the chemical industry. Chem. Eng. Trans. 2011; 24: 301-306.