Probing the effects of synthesized silver nanowire/reduced graphene oxide composites on the structure and esterase-like activity of human serum albumin and its impacts on human endometrial stem cells: A new platform in nanomedicine

Document Type : Research Paper

Authors

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu 30013, Taiwan

3 Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan

Abstract

Objective(s): Nowadays, the unique and fascinating properties of graphene‐based nanocomposites make them one of the most promising materials for therapeutics, delivery carriers as well as tissue engineering. On the other hand, silver nanowire has been attracting more attention in nanomedicine applications, too. In this study, the effects of synthesized silver nanowire/reduced graphene oxide (AgNWs/rGO) composites on the structure and esterase-like activity of Human Serum Albumin (HSA), as well as its impacts on Human Endometrial Stem Cells (hEnSCs), were evaluated
Materials and Methods: AgNWs/rGO composite was first synthesized and fabricated. Subsequently, its effects on the structure and esterase-like activity of HSA were evaluated by UV-Visible spectroscopy, circular dichroism spectroscopy, and fluorescence spectroscopy. Afterward, its impacts on the viability and growth of hEnSCs were studied by MTT assay, DAPI staining, and flow cytometry analysis.
Results: The spectroscopic results showed that AgNWs/rGO composite could form a complex with HSA, however, did not affect the secondary structure of HSA and the binding constant for this complex was found to be 5.4×104 mL.mg-1. Furthermore, HSA maintained most of its activity in the presence of the AgNWs/rGO composite. Based on FRET (fluorescence resonance energy transfer) data the value of r0 was less than 7 nm signifying that the energy transfer from HSA to AgNWs/rGO composite occurs with a high level of possibility. The MTT assay, DAPI staining, and flow cytometry analysis indicated that the AgNWs/rGO composite was non-toxic towards hEnSCs.
Conclusion: Our results suggest that the prepared AgNWs/rGO composite, potentially, is suitable in nanomedicine applications such as tissue engineering and drug delivery.

Keywords

References

1.Shin SR, Li Y-C, Jang HL, Khoshakhlagh P, Akbari M, Nasajpour A, Zhang YS, Tamayol A, Khademhosseini A. Graphene-based materials for tissue engineering. Adv Drug Deliv Rev. 2016; 105: 255-274.
2.Roy I, Rana D, Sarkar G, Bhattacharyya A, Saha NR, Mondal S, Pattanayak S, Chattopadhyay S, Chattopadhyay D. Physical and electrochemical characterization of reduced graphene oxide/silver nanocomposites synthesized by adopting a green approach. RSC Adv. 2015; 5(32): 25357-25364.
3.Yang K, Feng L, Shi X, Liu Z. Nano-graphene in biomedicine: theranostic applications. Chem Soc Rev. 2013; 42(2): 530-547.
4.Chung C, Kim Y-K, Shin D, Ryoo S-R, Hong BH, Min D-H. Biomedical applications of graphene and graphene oxide. Acc Chem Res. 2013; 46(10): 2211-2224.
5.Hatamie S, Ahadian MM, Zomorod MS, Torabi S, Babaie A, Hosseinzadeh S, Soleimani M, Hatami N, Wei Z-H. Antibacterial properties of nanoporous graphene oxide/cobalt metal organic framework. Mater Sci Eng C. 2019; 104: 109862.
6.Hatamie S, Mohamadyar-Toupkanlou F, Mirzaei S, Ahadian MM, Hosseinzadeh S, Soleimani M, Sheu WJ, Wei ZH, Hsieh TF, Chang WC, Wang CL. Cellulose acetate/magnetic graphene nanofiber in enhanced human mesenchymal stem cells osteogenic differentiation under alternative current magnetic field. Spin. 2019; 9(2): 1940011.
7.Nazari H, Azadi S, Hatamie S, Zomorrod MS, Ashtari K, Soleimani M, Hosseinzadeh S. Fabrication of graphene‐silver/polyurethane nanofibrous scaffolds for cardiac tissue engineering. Polym Adv Technol. 2019; 30(8): 2086-2099.
8.Hatamie S, Akhavan O, Sadrnezhaad SK, Ahadian MM, Shirolkar MM, Wang HQ. Curcumin-reduced graphene oxide sheets and their effects on human breast cancer cells. Mater Sci Eng C. 2015; 55: 482-489.
9.Yang K, Wan J, Zhang S, Zhang Y, Lee S, Liu Z. Long term biodistribution and toxicology of pegylated graphene in mice Parmacokinetic vivo, editor. ACS Nano. 2011; 5: 516-544.
10.He Y, Jiao B, Tang H. Interaction of single-stranded DNA with graphene oxide: fluorescence study and its application for S1 nuclease detection. RSC Adv. 2014; 4(35): 18294-18300.
11.Sun B, Zhang Y, Chen W, Wang K, Zhu L. Concentration dependent effects of bovine serum albumin on graphene oxide colloidal stability in aquatic environment. Environ Sci Technol. 2018; 52(13): 7212-7219.
12.Hekmat A, Saboury AA, Divsalar A. The effects of silver nanoparticles and doxorubicin combination on DNA structure and its antiproliferative effect against T47D and MCF7 cell lines. J Biomed Nanotechnol. 2012; 8(6): 968-982.
13.Xia Y, Yang P, Sun Y, Wu Y, Mayers B, Gates B, Yin Y, Kim F, Yan H. One‐dimensional nanostructures: synthesis, characterization, and applications. Adv Mater. 2003; 15(5): 353-389.
14.Zhang Y, Xu J, Yang Y, Sun B, Wang K, Zhu L. Impacts of proteins on dissolution and sulfidation of silver nanowires in aquatic environment: Importance of surface charges. Environ Sci Technol. 2020; 54: 5560–5568.
15.Hekmat A, Salavati F, Tackallou SH. The Effects of Paclitaxel in the Combination of Diamond Nanoparticles on the Structure of Human Serum Albumin (HSA) and Their Antiproliferative Role on MDA-MB-231cells. Protein J. 2020; 39: 268-283.
16.Liu Z, Chen X. Simple bioconjugate chemistry serves great clinical advances: albumin as a versatile platform for diagnosis and precision therapy. Chem Soc Rev. 2016; 45(5): 1432-1456.
17.Purcell M, Neault JF, Tajmir-Riahi HA. Interaction of taxol with human serum albumin. Biochim Biophys Acta. 2000; 1478(1): 61-68.
18.Mohamadi F, Ebrahimi-Barough S, Nourani MR, Mansoori K, Salehi M, Alizadeh AA, Tavangar SM, Sefat F, Sharifi S, Ai J. Enhanced sciatic nerve regeneration by human endometrial stem cells in an electrospun poly (ε-caprolactone)/collagen/NBG nerve conduit in rat. Artif Cells Nanomed Biotechnol. 2018; 46(8): 1731-1743.
19.Mobarakeh ZT, Ai J, Yazdani F, Sorkhabadi SMR, Ghanbari Z, Javidan AN, Mortazavi-Tabatabaei SA, Massumi M, Barough SE. Human endometrial stem cells as a new source for programming to neural cells. Cell Biol Int Rep. 2012; 19(1): 7-14.
20.Zhang Y, Xu J, Yang Y, Sun B, Wang K, Zhu L. Impacts of Proteins on Dissolution and Sulfidation of Silver Nanowires in an Aquatic Environment: Importance of Surface Charges. Environ Sci Technol. 2020; 54(9): 5560-5568.
21.Zhang L, Zhu W, Huang Y, Qi S. Synergetic Effects of Silver Nanowires and Graphene Oxide on Thermal Conductivity of Epoxy Composites. Nanomaterials. 2019; 9(9): 1264.
22.Saini P, Sharma R, Chadha N. Determination of defect density, crystallite size and number of graphene layers in graphene analogues using X-ray diffraction and Raman spectroscopy. Indian J Pure Ap Phy. 2017; 55(9): 625-629.
23.Maurya N, Maurya JK, Singh UK, Dohare R, Zafaryab M, Moshahid Alam Rizvi M, Kumari M, Patel R. In vitro cytotoxicity and interaction of noscapine with human serum albumin: Effect on structure and esterase activity of HSA. Mol Pharm. 2019; 16(3): 952-966.
24.Tran QT, Huynh TMH, Tong DT, Nguyen ND. Synthesis and application of graphene–silver nanowires composite for ammonia gas sensing. Adv Nat Sci-Nanosci. 2013; 4(4): 045012.
25.Fallahi H, Azizi H, Ghasemi I, Karrabi M. Preparation and properties of electrically conductive, flexible and transparent silver nanowire/poly (lactic acid) nanocomposites. Org Electron. 2017; 44: 74-84.
26.Yousef S, Mohamed A, Tatariants M. Mass production of graphene nanosheets by multi-roll milling technique. Tribol Int. 2018; 121: 54-63.
27.Wang Y, Wang X, Wang J, Zhao Y, He W, Guo Z. Noncovalent interactions between a trinuclear monofunctional platinum complex and human serum albumin. Inorg Chem. 2011; 50(24): 12661-12668.
28.Amani N, Saberi MR, Khan Chamani J. Investigation by fluorescence spectroscopy, resonance rayleigh scattering and zeta potential approaches of the separate and simultaneous binding effect of paclitaxel and estradiol with human serum albumin. Protein Peptide Lett. 2011; 18(9): 935-951.
29.Chaves OA, Amorim APdO, Castro LH, Sant’Anna CMR, De Oliveira MC, Cesarin-Sobrinho D, et al. Fluorescence and docking studies of the interaction between human serum albumin and pheophytin. Molecules. 2015; 20(10): 19526-19539.
30.Nan Z, Hao C, Ye X, Feng Y, Sun R. Interaction of graphene oxide with bovine serum albumin: A fluorescence quenching study. Spectrochimica Acta A Mol Biomol Spectrosc. 2019; 210: 348-354.
31.Zhang H, Liu W, Yang L, Liu J, Wang Y, Mao X, Wang J, Xu X. Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA. J Nanomater. 2019; 2019: 2707909.
32.Ranjbar S, Shokoohinia Y, Ghobadi S, Bijari N, Gholamzadeh S, Moradi N, Ashrafi-Kooshk MR, Aghaei A, Khodarahmi R. Studies of the interaction between isoimperatorin and human serum albumin by multispectroscopic method: identification of possible binding site of the compound using esterase activity of the protein. Sci World J. 2013; 2013: 305081.
33.Hekmat A, Saboury AA, Divsalar A, Seyedarabi A. Structural effects of TiO2 nanoparticles and doxorubicin on DNA and their antiproliferative roles in T47D and MCF7 cells. Anticancer Agents Med Chem. 2013; 13(6): 932-951.
34.Zhao X, Liu R, Chi Z, Teng Y, Qin P. New insights into the behavior of bovine serum albumin adsorbed onto carbon nanotubes: comprehensive spectroscopic studies. J Biomed Opt. 2010; 114(16): 5625-5631.
35.Masters B. Principles of Fluorescence Spectroscopy, Third Edition. J Biomed Opt. 2008; 13(2): 029901.
36.Lu Q, Chen C, Zhao S, Ge F, Liu D. Investigation of the interaction between gallic Acid and α-amylase by spectroscopy. Int J Food Prop. 2016; 19(11): 2481-2494.
37.Luo H, Song C, Lin X, Peng Z, Weng L, Tang X, Xu S, Song M, Jin L, Zheng X. Study on AgInZnS-graphene oxide non-toxic quantum dots for biomedical sensing. Front Chem. 2020; 8: 331.
38.Hekmat A, Hajebrahimi Z, Motamedzade A. Structural Changes of Human Serum Albumin (HSA) in Simulated Microgravity. Protein Peptide Lett. 2017; 24(11): 1030-1039.
39.Corrêa DH, Ramos CH. The use of circular dichroism spectroscopy to study protein folding, form and function. Afr J Biomed Res. 2009; 3(5): 164-173.
40.Fu X-B, Liu D-D, Lin Y, Hu W, Mao Z-W, Le X-Y. Water-soluble DNA minor groove binders as potential chemotherapeutic agents: synthesis, characterization, DNA binding and cleavage, antioxidation, cytotoxicity and HSA interactions. Dalton Trans. 2014; 43(23): 8721-8737.
41.Ghosh D, Dey SK, Saha C. Mutation induced conformational changes in genomic DNA from cancerous K562 cells influence drug-DNA binding modes. PLoS One. 2014; 9(1): e84880.
42.Sekowski S, Kazmierczak A, Mazur J, Przybyszewska M, Zaborski M, Shcharbin D, Gabryelak T. The interaction between PAMAM G3. 5 dendrimer, Cd2+, dendrimer–Cd2+ complexes and human serum albumin. Colloids Surf B. 2009; 69(1): 95-98.
43.Kubiak-Ossowska K, Jachimska B, Mulheran PA. How negatively charged proteins adsorb to negatively charged surfaces: a molecular dynamics study of BSA adsorption on silica. J Phys Chem B. 2016; 120(40): 10463-10468.
44.Lehmann SG, Toybou D, del Real A-EP, Arndt D, Tagmount A, Viau M, Safi M, Pacureanu A, Cloetens P, Bohic S, Salomé M, Castillo-Michel H, Omaña-Sanz B, Hofmann A, Vulpe C, Simonato J-P, Celle C, Charlet L, Gilbert B. Crumpling of silver nanowires by endolysosomes strongly reduces toxicity. Proc Natl Acad Sci. 2019; 116(30): 14893-14898.
45.An HJ, Sarkheil M, Park HS, Yu IJ, Johari SA. Comparative toxicity of silver nanoparticles (AgNPs) and silver nanowires (AgNWs) on saltwater microcrustacean, Artemia salina. Comp Biochem Phys C. 2019; 218: 62-69.
46.Wang F, Wang Y, Qu G, Yao X, Ma C, Song M, Wang H, Jiang G. Ultralong AgNWs-induced toxicity in A549 cells and the important roles of ROS and autophagy. Ecotoxicol Environ Saf. 2019; 186: 109742.
Nanomedicine Journal
Volume 8, Issue 1
January 2021
Pages 42-56

Files

Share

How to cite

Statistics