Antibacterial activity of green synthesized silver nanoparticles using Pistacia hull against multidrug-resistant clinical isolates

Document Type : Research Paper

Authors

1 Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

2 Department of Biotechnology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran

3 Core Research Facilities, Isfahan University of Medical Sciences, Isfahan, Iran

4 Nosocomial Infection Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

5 Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

6 Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Wroclaw, Poland

Abstract

Objective(s): Silver nanoparticles (AgNPs) can be considered as the new antibacterial agents. The antibacterial effects of synthesized AgNPs from Iranian pistachio hulls on several antibiotic-resistant bacteria were assessed in this study. 
Materials and Methods: In an experimental study, AgNPs were synthesized by reducing Ag+ ions using pistachio hulls. Several methods characterized the qualities of AgNPs. Antibacterial activities of the AgNPs against six gram-positive and gram-negative standard bacteria and 30 multidrug-resistant (MDR) clinical isolates were investigated by well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. 
Results: The aqueous extract of pistachio hulls had an acceptable potential to synthesize AgNPs, and the formed nanoparticles displayed suitable size and acceptable stability in solutions. Antibacterial activities of the AgNPs were detected against two standard strains, Escherichia coli, and Staphylococcus aureus, with growth inhibition zones of 13 and 11 mm, respectively. MIC were 10 mg/ml for E. coli and 20 mg/ml for S. aureus. MBC for both bacteria was the same as MIC. MIC and MBC AgNPs against 15 methicillin-resistant S. aureus (MRSA) isolates ranged from 40 to 10 mg/ml. In extended-spectrum beta-lactamase (ESBL) E. coli isolates, 11 and 3 isolates have MIC equal to 20 and 10 mg/ml, respectively. Three ESBL E. coli isolates had 10, 5 and 2.5 mg/ml MBC; in other isolates, MBC and MIC were the same. 
Conclusion: The green synthesis of AgNPs using pistachio hull can replace common chemical and physical methods. AgNPs displayed antibacterial activities, and they could replace some antibiotics. 

Keywords

References

1. Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, et al. The bactericidal effect of silver nanoparticles. Nanotechnology. 2005; 16(10):2346-2353
2. Organization WHO. WHO publishes list of bacteria for which new antibiotics are urgently needed. Geneva, Switzerland: WHO; 2017. 2019.
3. Shrivastava SR, Shrivastava PS, Ramasamy J. World health organization releases global priority list of antibiotic - resistant bacteria to guide research, discovery, and development of new antibiotics. J Med Soc. 2018;32(1):76-77.
4. Guo Z, Chen Y, Wang Y, Jiang H, Wang X. Advances and challenges in metallic nanomaterial synthesis and antibacterial applications. J Mater Chem B. 2020;8 (22): 4764-77
5. Pelgrift RY, Friedman AJ. Nanotechnology as a therapeutic tool to combat microbial resistance. Adv Drug Deliv Rev. 2013;65(13-14):1803-1815.
6. Holm VR, Greve MM, Holst B. A theoretical investigation of the optical properties of metal nanoparticles in water for photo thermal conversion enhancement. Energy Convers Manag. 2017; 149:536-542.
7. Khan SA. Metal nanoparticles toxicity: Role of physicochemical aspects. In Metal nanoparticles for drug delivery and diagnostic applications. Micro and Nano Technologies. 2020; 1-11. 
8. Srinoi P, Chen Y-T, Vittur V, Marquez MD, Lee TR. Bimetallic nanoparticles: enhanced magnetic and optical properties for emerging biological applications. Appl Sci. 2018;8(7):1106.
9. Yuan P, Ding X, Yang YY, Xu QH. Metal nanoparticles for diagnosis and therapy of bacterial infection. Adv Healthc Mater. 2018;7(13):1701392.
10. Allafchian A, Mirahmadi-Zare S, Jalali S, Hashemi S, Vahabi M. Green synthesis of silver nanoparticles using phlomis leaf extract and investigation of their antibacterial activity. J Nanostructure Chem. 2016; 6:129-135.
11. Elumalai E, Prasad T, Hemachandran J, Therasa SV, Thirumalai T, David E. Extracellular synthesis of silver nanoparticles using leaves of Euphorbia hirta and their antibacterial activities. J Pharm Sci Res. 2010;2(9):549-554.
12. Sadeghi B, Rostami A, Momeni S. Facile green synthesis of silver nanoparticles using seed aqueous extract of Pistacia atlantica and its antibacterial activity. Spectrochim. Acta A Mol. Biomol. Spectrosc. 2015; 134:326-332.
13. Golabiazar R, Othman KI, Khalid KM, Maruf DH, Aulla SM, Yusif PA. Green synthesis, characterization, and investigation antibacterial activity of silver nanoparticles using Pistacia atlantica leaf extract. Bionanoscience. 2019;9(2):323-333.
14. Ahmed ZB, Yousfi M, Viaene J, Dejaegher B, Demeyer K, Mangelings D, et al. Antioxidant activities of Pistacia atlantica extracts modeled as a function of chromatographic fingerprints in order to identify antioxidant markers. Microchem J. 2016; 128:208-217.
15. Samavati V, Adeli M. Isolation and characterization of hydrophobic compounds from carbohydrate matrix of Pistacia atlantica. Carbohydr. Polym.. 2014; 101:890- 896.
16. Fanoro OT, Oluwafemi OS. Bactericidal antibacterial mechanism of plant synthesized silver, gold and bimetallic nanoparticles. Pharmaceutics. 2020;12(11):1044.
17. Gharibi S, Tabatabaei BES, Saeidi G, Talebi M, Matkowski A. The effect of drought stress on polyphenolic compounds and expression of flavonoid biosynthesis related genes in Achillea pachycephala Rech. f. Phytochemistry. 2019; 162:90-98.
18. Jansen W, Van der Bruggen J, Verhoef J, Fluit A. Bacterial resistance: A sensitive issue: Complexity of the challenge and containment strategy in Europe. Drug Resist Updat. 2006;9(3):123-133.
19. Shimanovich U, Gedanken A. Nanotechnology solutions to restore antibiotic activity. J Mater Chem B. 2016;4(5):824-833.
20. Gharibi S, Matkowski A, Sarfaraz D, Mirhendi H, Fakhim H, Szumny A, et al. Identification of polyphenolic compounds responsible for antioxidant, anti-candida activities and nutritional properties in different pistachio (Pistacia vera) hull cultivars. Molecules. 2023;28(12):4772.
21. Bellocco E, Barreca D, Laganà G, Calderaro A, El Lekhlifi Z, Chebaibi S, et al. Cyanidin-3-O-galactoside in ripe pistachio (Pistachia vera L. variety Bronte) hulls: Identification and evaluation of its antioxidant and cytoprotective activities. J Funct. 2016; 27:376-835.
22. Kashi R, Bagheri-Mohagheghi M, Khorshidi M. Synthesis and study of structural, optical, and antibacterial properties of silver, copper, and iron metallic nanoparticles prepared by green synthesis. Appl Nanosci. 2023;13(6):4343-4360.
23. He Y, Ingudam S, Reed S, Gehring A, Strobaugh TP, Irwin P. Study on the mechanism of antibacterial action of magnesium oxide nanoparticles against foodborne pathogens. J Nanobiotechnology. 2016; 14:1-9.
24. Yousefi A, Seyyed Ebrahimi S, Seyfoori A, Mahmoodzadeh Hosseini H. Maghemite nanorods and nanospheres: synthesis and comparative physical and biological properties. BioNanoScience. 2018; 8:95-104.
25. Breijyeh Z, Jubeh B, Karaman R. Resistance of gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules. 2020;25(6):1340.
26. Shah IH, Ashraf M, Sabir IA, Manzoor MA, Malik MS, Gulzar S, et al. Green synthesis and Characterization of Copper oxide nanoparticles using Calotropis procera leaf extract and their different biological potentials. J Mol Struct. 2022; 1259:132696.
27. Manso T, Lores M, de Miguel T. Antimicrobial activity of polyphenols and natural polyphenolic extracts on clinical isolates. Antibiotics. 2021;11(1):46.
28. Bhattacharya D, Bhattacharya S, Patra MM, Chakravorty S, Sarkar S, Chakraborty W, et al. Antibacterial activity of polyphenolic fraction of kombucha against enteric bacterial pathogens. Curr Microbiol. 2016; 73:885-896.
29. De Araújo AA, Soares LAL, Ferreira MRA, de Souza Neto MA, da Silva GR, de Araújo Jr RF, et al. Quantification of polyphenols and evaluation of antimicrobial, analgesic and anti-inflammatory activities of aqueous and acetone– water extracts of Libidibia ferrea, Parapiptadenia rigida and Psidium guajava. J Ethnopharmacol. 2014; 156:88-96.
30. Behravan M, Panahi AH, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using Berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int J Biol Macromol. 2019; 124:148-154.
31. Bagherzade G, Tavakoli MM, Namaei MH. Green synthesis of silver nanoparticles using aqueous extract of saffron (Crocus sativus L.) wastages and its antibacterial activity against six bacteria. Asian Pac J Trop Biomed. 2017;7(3):227-233.
32. Rahimi F, Bouzari M, Katouli M, Pourshafie MR. Antibiotic resistance pattern of methicillin resistant and methicillin sensitive Staphylococcus aureus isolates in Tehran, Iran. Jundishapur J Microbiol. 2013;6(2):144-149.
33. Niakan S, Niakan M, Hesaraki S, Nejadmoghaddam MR, Moradi M, Hanafiabdar M, et al. Comparison of the antibacterial effects of nanosilver with 18 antibiotics on multidrug resistance clinical isolates of Acinetobacter baumannii. Jundishapur J Microbiol. 2013;6(5):e8341.
 
 
Nanomedicine Journal
Volume 11, Issue 4
October 2024
Pages 450-457

Files

Share

How to cite

Statistics