Streptomyces somaliensis mediated green synthesis of silver nanoparticles

Document Type : Research Paper


1 Department of Plant Pathology, College of Agriculture, Shahid Bahonar University of Kerman, Iran

2 Department of Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Iran


The development of reliable and ecofriendly process for the synthesis of nano-metals is an important aspect in the field of nanotechnology. Nano-metals are a special group of materials with broad area of applications.

Materials and Methods:
In this study, extracellular synthesis of silver nanoparticles (SNPs) performed by use of the gram positive soil Streptomycetes. Streptomycetes isolated from rice fields of Guilan Province, Iran (5 isolates). Initial characterization of SNPs was performed by visual change color. To determine the bacterium taxonomical identity, its colonies characterized morphologically by use of scanning electron microscope. The PCR molecular analysis of active isolate represented its identity partially. In this regard, 16S rDNA of isolate G was amplified using universal bacterial primers FD1 and RP2. The PCR products were purified and sequenced. Sequence analysis of 16S rDNA was then conducted using NCBI GenBank database using BLAST. Also SNPs were characterized by, transmission electron microscopy (TEM) and X-ray diffraction spectroscopy (XRD).

From all 5 collected Streptomyces somaliensis isolates, isolate G showed highest extracellular synthesis of SNPs via in vitro. SNPs were formed immediately by the addition of (AgNO3) solution (1 mM). UV-visible spectrophotometry for measuring surface plasmon resonance showed a single absorption peak at 450 nm, which confirmed the presence of SNPs. TEM revealed the extracellular formation of spherical silver nanoparticles in the size range of 5-35 nm.

The biological approach for the synthesis of metal nanoparticles offers an environmentally benign alternative to the traditional chemical and physical synthesis methods. So, a simple, environmentally friendly and cost-effective method has been developed to synthesize AgNPs using Streptomycetes.


1. Mohanpuria P, Rana KN, Yadav SK. Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart. Res. 2008; 10: 507–517.
2. Matei A, Cernica I, Cadar O, Roman C, Schiopu V,.Synthesis and characterization of ZnO–polymer nanocomposites. J Mater Form. 2008; 1: 767–770.
3. Haberland H. Clusters of Atoms and Molecules, Springer series in chemical physics, Berlin, New York. 1994.
4. Shahidi Bonjar L. Nanogold detoxifying machine” to remove idle nanogold particles from blood stream of cancer patients treated with antibody-nanogold therapeutics. Med hypotheses. 2013; 80(5): 601–605.
5. Chaloupka K, Malam Y, Seifalian AM. Nanosilver as a new generation of nanoproduct in biomedical applications. Trends Biotechnol. 2010; 28:580–8.
6. Adam S ,Gabriela K, Ivo Š, Mirka Š, Ivan R, Leslie MS. Applications of biosynthesized metallic nanoparticles – A review. Acta Biomater. 2014; 10: 4023–4042
7. Valodkar M, Bhadorai A, Pohnerkar J, Mohan M,  Thakore S. Morphology and antibacterial activity of carbohydrate stabilized silver nanoparticles. Carbohydr Res. 2010; 345: 1767–1773.
8. Noura E, Ahmady N,  Nayera A, Abdelwahed M. Application of Statistical Experimental Design for Optimization of Silver Nanoparticles Biosynthesis by a Nanofactory Streptomyces viridochromogenes. J Microbiol. 2014; 52: 53–63.
9. Yaqiong Q, Xiaohui J, Jing J, Hong L, Hongli W, Wensheng Y. Size control over spherical silver nanoparticles by ascorbic acid reduction. Colloid Surf. 2010; 372: 172–176.
10. Khatami M, Pourseyedi S, Khatami M, Hamidi H, Zaeifi M, Soltani L. Synthesis of silver nanoparticles using seed exudates of Sinapis arvensis as a novel bioresource, and evaluation of their antifungal activity.  Bioresour and Bioprocessing.  2015; 2.
11. Nishant S, Mausumi M. Biosynthesis and Characterization of Gold Nanoparticles Using Zooglea ramigera and Assessment of Its Antibacterial Property. J Clust Sci. 2014; 26:1-18
12. Mingguo W, Guoqing Li, Jibin Zh, Daohong J, Hung-Chang Hu. Effect of volatile substances of Streptomyces platensis F-1 on control of plant fungal diseases. Biol Control. 2008; 46: 552–559.
13. Soltani Nejad M, Shahidi Bonjar GH,  Khaleghi N.Biosynthesis of gold nanoparticles using Streptomyces fulvissimus isolate U. Nanomed J. 2015; 2: 153-159.
14. Lee JY, Hwang BK. Diversity of antifungal Actinomycetes in various vegetative soils of Korea. Can J Microbiol. 2002; 48: 407-417.
15. Ebrahimi Zarandi M, Shahidi Bonjar GH,. Padasht Dehkaei F, Ayatollahi Moosavi SA, Rashid Farokhi P, Aghighi S. Biological Control of Rice Blast (Magnaporthe oryzae) by use of Streptomyces sindeneusis isolate 263 in Greenhouse. Am J  Appl Sci. 2009: 6: 194-199.
16. Kim HJ, Lee SC, Hwang BK. Streptomyces cheonanensis sp. Nov., a novel streptomycete with antifungal activity. Int. J. Syst. Evol Microbiol. 2006; 56: 471–475.
17. Anuradha P, Seema S, Naheed A, Ashok G, Preety S. Synthesis of AgNPs by Bacillus Cereus Bacteria and Their Antimicrobial Potential. J Biomater Nanobiotechnol. 2011; 2: 156-162.
18. Gaikwad S, Bhosale A. Green Synthesis of Silver Nanoparticles Using Aspergillus niger and its Efficacy Against Human Pathogens. Eur J Exp Biol. 2012; 2 :1654-1658.
19. Khatami M, Pourseyedi S. Phoenix dactylifera (date palm) pit aqueous extract mediated novel route for synthesis high stable AgNPs with high antifungal and antibacterial activity, IET Nanobiotechnol. 2015; 1-7.